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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2025

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Introduction: Hepatitis B Virus (HBV) infection is still an ongoing public health issue worldwide. The most efficient tool in preventing HBV infection remains vaccination and significant efforts have been made in the last decade to improve current HBV vaccines. Owing to the strict HBV tropism for the human liver, developing animal models for preclinical screening of vaccine candidates is extremely challenging. To date, there are only a few reports regarding the use of humanized mouse models for the evaluation of the immunogenic properties of viral antigens.Methods: Previously we showed that a Nicotiana benthamiana-produced HBV-S/preS116-42 antigen elicited strong HBV-specific immune responses in BALB/c mice. In the current study, we used immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice as recipients of human peripheral blood mononuclear cells (hPBMCs), to evaluate the immunogenicity of the recently developed chimeric HBV immunogen produced in CRISPR/Cas9-edited N. benthamiana, under more “humanized” conditions.Results: Analysis of the immune response in NSG mice immunized with the chimeric antigen demonstrated induction of virus infection-neutralizing antibodies, indicating activation of antigen-specific B cells.Discussion: The ability of hPBMCs-engrafted NSG mice to mount specific humoral immune responses after immunization with viral antigens supports this animal model as a promising tool for pre-clinical evaluation of human vaccine antigens.

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Metabarcoding targeting nematodes, bacteria, fungi and oomycetes was used in combination with multispectral drone imagery and traditional soil extraction of nematodes to diagnose poor growth in patches of a potato field in Norway. Areas of good and poor growth as identified by the normalised difference vegetation index (NDVI) based on aerial photography were compared, and nematodes were identified as the likely drivers of poor growth. This was based on the presence of known plant-parasitic nematodes in the field and the significant association between low alpha diversity (total genus richness and abundance) for nematodes with areas of poor growth, while alpha diversity for other organism groups did not vary between patches with good and poor growth. Metabarcoding represented nematodes well compared to traditional soil extraction. The combination of aerial photography and metabarcoding used in this work offers a promising possibility to identify biological drivers of growth differences across organism groups at field scale.

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Low pollinator richness and abundance is a primary driver of pollination deficits and may lead to reduced yields (production deficits). In response, domesticated honeybees are often used to increase pollination success, even though honeybees are less efficient pollinators than naturally occurring wild bees. Here, we explored whether Norwegian apple orchards experience pollination and production deficits, and if such deficits could be related to specific pollinator groups and activity. We conducted a supplemental pollination experiment and measured seed set and yield (fruit set x weight) for three cultivars, in six orchards, in two distinct apple growing regions in central Norway, for two years. In addition, we used cameras to record relative pollinator activity throughout the flowering period. Overall, we found a pollination and production deficit across all cultivars, although there were differences in pollination deficit among cultivars. Three orchards had a pollination deficit both years of the study, suggesting sub-optimal orchard structure and/or a lack of pollinators. However, we found that solitary bees significantly reduced both pollination and production deficit, suggesting that orchard management actions should focus on increasing wild bee diversity and abundance.