Kari Skjånes
Forsker
Lenker
ResearchGateBiografi
Kari har doktorgrad innen mikroalgeteknologi fra UiB, med hovedvekt på dyrkingsteknologi. Forskningen har fokus på mikroalger til bruk i mat og fôr, der dyrkingsmetoder og stressfysiologi blir brukt til å fremstille algebiomasse med komposisjon som er tilpasset bruk i spesifikke produkter. Mikroalger som kilde til protein, fettsyrer (PUFA) og pigmenter (f.eks. karotenoider), er viktige elementer. NIBIOs pilotanlegg for mikroalger på Vollebekk på Ås med storskala fotobioreaktorer blir brukt til å forske på oppskalering av dyrkingsmetoder som er utviklet i labskala, og til produksjon av algebiomasse for utvikling av ulike mikroalgeprodukter. Hun jobber i tillegg med psykrofile mikroalger og andre alger tilpasset lave temperaturer, som kilde til bioaktive stoffer gjennom bioprospektering.
Sammendrag
Algal-based wastewater remediation systems (phycoremediation) include phycosphere bacterial communities that influence algal growth, pollutant remediation, and downstream applications of biomass as fertilizers or bio-stimulants. This study investigated the bacterial community dynamics in a novel phycoremediation system using a co-culture of the green algae Stigeoclonium sp. and Oedogonium vaucheri. Bacterial abundance was estimated using flow cytometry (FCM), while community composition was assessed through 16S rRNA gene metabarcoding. Additionally, 28 bacterial strains were isolated from the bioremediation experiment, cultured, genetically characterized for identification and screened for production of the auxin phytohormone indole-3-acetic acid (IAA). Metabarcoding showed that the free-living bacterial community consisted of bacteria from both the wastewater effluent and the algal inocula, while the attached phycosphere community was dominated by bacteria from the algal inocula, indicating the stability of the algae-associated phycosphere. Taxa known to include plant growth-promoting bacteria (PGPB) were abundant, and several strains produced IAA. The bacterial community composition, combined with the potential production of phytohormone by isolated bacteria indicates symbiotic or commensal algae-microbe interactions within the phycosphere bacterial communities. Sterile filtration of wastewater effluent, including only the algal inoculum bacterial communities, reduced algal biomass production and increased bacterial abundance. This study highlights the critical role of microbial interactions in engineered ecosystems and provides insights for optimizing algal-based wastewater treatment technologies.
Forfattere
Ralf Rautenberger Alexandre Detain Kari Skjånes Peter Simon Claus Schulze Viswanath Kiron Daniela Morales-SánchezSammendrag
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Sammendrag
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