Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2024
Abstract
Det er ikke registrert sammendrag
Authors
Alois Bilavčík Stacy Denise Hammond Hammond Jana Fránová Igor Koloniuk Zhibo Hamborg Dag-Ragnar Blystad Milos Faltus Jiri ZamecnikAbstract
This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free procedure using two raspberry varieties. Regrowth rate of the raspberry variety ‘Tulameen’ after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety ‘Autumn First’, the regrowth level was 73%. In vitro shoots of the cryopreserved variety ‘Tulameen’ were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of ‘Tulameen’ by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.
Authors
Xiao-Yan Ma Dag-Ragnar Blystad Qiao-Chun Wang Lu Tong Øyvor Stensbøl Dong Zhang Zhibo HamborgAbstract
By optimizing size of shoot tips, preculture medium and exposure duration to PVS2, we established an efficient and wide-spectrum droplet-vitrification cryopreservation for shoot tips of raspberry (Rubus idaeus L.). This protocol yielded 80–100% and 67–100% of survival and shoot regrowth levels in cryopreserved shoot tips across 23 raspberry genotypes. Genetic integrity was assessed in cryo-derived regenerants after 3 months of post-cryopreservation using inter simple sequence repeat (ISSR), single nucleotide polymorphism (SNPs), and insertions and deletions (InDels). ISSR did not detect any polymorphic bands in the cryo-derived regenerants. Although the number of SNPs and InDels decreased in the cryo-derived regenerants, variation trends were similar between the cryo-derived regenerants and the control. Plant vegetative growth and root growth were assessed in the cryo-derived plants after 9 weeks of growth in greenhouse. There were no significant differences in plant vegetative growth measured by plant height, number of fully-opened leaved, leaf area, and fresh and dry weight between the cryo-derived plants and the control, although significant differences were observed in root growth measured by root total length, root average diameter and root volume between the two types of plants. The results obtained in the present study indicate that the droplet-vitrification method has great potential for cryopreservation of raspberry germplasm.
Authors
Ondrej Lenz Igor Koloniuk Tatiana Sarkisova Radek Cmejla Lucie Valentova Martina Rejlova Jiri Sedlak Dag-Ragnar Blystad Bijaya Sapkota Zhibo Hamborg Jiunn Luh Tan Rostislav Zemek Pribylová Jaroslava Jana FranovaAbstract
A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants in the Czech Republic and has tentatively been named raspberry rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within the group, albeit distantly related to other members. A screening of 679 plant and 168 arthropod samples from the Czech Republic and Norway revealed RaRV1 in 10 raspberry shrubs, one batch of Aphis idaei, and one individual of Orius minutus. Furthermore, a distinct isolate of this virus was found, sharing 95% amino acid identity in both the full nucleoprotein and partial sequence of the RNA-dependent RNA polymerase gene sequences, meeting the species demarcation criteria. This discovery marks the first reported instance of a rubodvirus infecting raspberry plants. Although transmission experiments under experimental conditions were unsuccessful, positive detection of the virus in some insects suggests their potential role as vectors for the virus.
Authors
Ioannis E. Tzanetakis V. Aknadibossian Josef Špak F. Constable S. J. Harper John Hammond Therry Candresse S. Y. Folimonova J. Freitas-Astua M. Fuchs W. Jelkmann V. I. Maliogka A. Marais R. R. Martin D. Mollov G. Vidalakis N. Aboughanem-Sabanadzovic M. Al Rwahnih O. J. Alabi D. Alioto H. Y. Atanda F. Bagi V. K. Baranwal C. Barbosa M. Bar-Joseph L. Batista Le Riverend T. Belien M. J. Beniıtez-Galeano H. Bennypaul A. Bertaccini R. Bester A. G. Blouin Dag-Ragnar Blystad M. Botermans O. Bozan A. Brakta Y. Brans A. Bulajic K. Caglayan A. Catara E. Choueiri M. Cieslinska G. Cook W. Cui J. da Graça S. Davino C. Delmiglio M. M. Dewdney F. Di Serio Zhibo HamborgAbstract
This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed by “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those agents remain on regulatory lists, creating barriers in trade and plant certification. Historically identified based solely on symptoms, these agents lack isolates or sequence data, making reliable detection or risk assessment impossible. After reviewing over 120 such agents across 10 key plant genera, we recommend their removal from regulatory lists and call for revised standards aligned with modern diagnostics. This effort seeks to streamline germplasm exchange, benefiting global agriculture by removing the constraints imposed by phantoms.
Abstract
Det er ikke registrert sammendrag
Abstract
Det er ikke registrert sammendrag
Abstract
Det er ikke registrert sammendrag
Abstract
Det er ikke registrert sammendrag
Abstract
Det er ikke registrert sammendrag