Dag-Ragnar Blystad
Seniorforsker
(+47) 908 72 588
dag-ragnar.blystad@nibio.no
Sted
Ås - Bygg H7
Besøksadresse
Høgskoleveien 7, 1433 Ås
Sammendrag
Planter er levested for mange mikroorganismer. Fytoplasmaer er små bakterier som mangler cellevegg, og de kan dermed ikke leve utenfor en plante eller en insektvektor. Mer enn hundre fytoplasma-arter er kjent, hvorav seks er påvist i Norge. De tre mest kjente her til lands fører til heksekost på eple, dvergsjuke i bringebær og pærevisnesjuke.
Forfattere
Lenz Ondrej Koloniuk Igor Sarkisova Tatiana Cmejla Radek Valentova Lucie Rejlova Martina Sedlak Jiri Dag-Ragnar Blystad Sapkota Bijaya Zhibo Hamborg Tan Jiunn Luh Zemek Rostislav Franova JanaSammendrag
A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants in the Czech Republic and has tentatively been named raspberry rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within the group, albeit distantly related to other members. A screening of 679 plant and 168 arthropod samples from the Czech Republic and Norway revealed RaRV1 in 10 raspberry shrubs, one batch of Aphis idaei, and one individual of Orius minutus. Furthermore, a distinct isolate of this virus was found, sharing 95% amino acid identity in both the full nucleoprotein and partial sequence of the RNA-dependent RNA polymerase gene sequences, meeting the species demarcation criteria. This discovery marks the first reported instance of a rubodvirus infecting raspberry plants. Although transmission experiments under experimental conditions were unsuccessful, positive detection of the virus in some insects suggests their potential role as vectors for the virus.
Forfattere
Jiunn Luh Tan Nina Trandem Zhibo Hamborg Bijaya Sapkota Dag-Ragnar Blystad Jana Fránová Rostislav ZemekSammendrag
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