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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2021

Sammendrag

I 2020 ble det analysert 1107 prøver av ferske, fryste eller bearbeidede matvarer fra konvensjonell produksjon og 167 prøver av økologiske matvarer i overvåkingsprogrammet «Rester av plantevernmidler i næringsmidler» som NIBIO utfører på oppdrag av og i samarbeid med Mattilsynet. Prøveuttaket omfattet 106 ulike vareslag fra 62 forskjellige land. Av de 1274 prøvene var 30 % norskproduserte næringsmidler, 35 % var produkter fra EU/EØS-land og 35 % av matvarene var importert fra tredjeland. Resultatene viser at innholdet av rester av plantevernmidler i næringsmidler på det norske markedet er lavt. I mer enn 97 % av prøvene som ble tatt ut i 2020 fra konvensjonell produksjon, er innholdet innenfor de fastsatte grenseverdiene. Det var funn over grenseverdi i to norske prøver, tre prøver fra EU/EØS-land og 22 prøver fra tredjeland.

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Sammendrag

Plant virus eradication is a prerequisite for the use of virus-free propagules for sustainable crop production. In contrast, virus preservation is required for all types of applied and basic research of viruses. Shoot tip cryopreservation can act as a double-edged strategy, facilitating either virus eradication or virus preservation in cryoderived plants. Here, we tested the efficacies of shoot tip cryopreservation for virus eradication and preservation in shallot (Allium cepa var. aggregatum). In vitro stock shallot shoots infected with onion yellow dwarf virus (OYDV) and shallot latent virus were thermotreated for 0, 2, and 4 weeks at a constant temperature of 36℃ before shoot tip cryopreservation. Results showed that viruses were preserved in recovered shoots when thermotherapy was not applied. Although thermotherapy lowered the regrowth levels of cryotreated shoot tips, the efficiency of virus eradication increased from 5% to 54%. Immunolocalization of OYDV and histological observation of cryotreated shoot tips showed the high frequency of virus preservation was due to the viral invasion of cells close to the apical meristem and the high proportion of cells surviving. Four weeks of thermotherapy drastically decreased the distribution of OYDV, as well as the percentage of surviving cells within the shoot tips, thereby promoting virus eradication. Virus-free plants obtained from combining thermotherapy with cryotherapy showed significantly improved vegetative growth and bulb production. The present study reports how thermotherapy can act as a trigger to facilitate either the safe preservation of Allium viruses or the production of virus-free shallot plants.

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Sammendrag

Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

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Sammendrag

(1) We document the invertebrate fauna collected from 24 oak canopies in east and west Norway as a contribution to the Norwegian Biodiversity Information Centre’s ‘The Norwegian Taxonomy Initiative’. (2) A snap-shot inventory of the canopies was recorded by means of emitting a mist of natural pyrethrum into the canopies at night using a petrol-driven fogger and collecting the specimens in butterfly nets spread on the ground under the canopy. (3) Almost the entire catch of more than 6800 specimens was identified to 722 species. Out of 92 species new to the Norwegian fauna, 21 were new to science and, additionally, 15 were new to the Nordic fauna. Diptera alone constituted nearly half of the species represented, with 61 new records (18 new species). Additionally, 24 Hymenoptera (one new species), six oribatid mites (two new species) and one Thysanoptera were new to the Norwegian fauna. (4) Our study emphasizes the importance of the oak tree as a habitat both for a specific fauna and occasional visitors, and it demonstrates that the canopy fogging technique is an efficient way to find the ‘hidden fauna’ of Norwegian forests. The low number of red listed species found reflects how poor the Norwegian insect fauna is still studied. Moreover, the implication of the IUCN red list criteria for newly described or newly observed species is discussed.

Sammendrag

Future weather patterns are expected to result in increased precipitation and temperature, in Northern Europe. These changes can potentially cause an increase in plant disease and insect pests which will alter agricultural practice amongst other things the used crop types and application patterns of pesticides. We use a Bayesian network to explore a probabilistic risk assessment approach to better account for variabilities and magnitudes of pesticide exposure to the aquatic ecosystem. As Bayesian networks link selected input and output variables from various models and other information sources, they can serve as meta-models. In this study, we are using a pesticide fate and transport models (e.g. WISPE) with specific environmental factors such as soil and site parameters together with chemical properties and climate scenarios that are linked to a representative Norwegian study area. The derived exposure of pesticide of the study area is integrated in the Bayesian network model to estimate the risk to the aquatic ecosystem also integrating an effect distribution derived from toxicity test. This Bayesian network model will allow to incorporate climate predictions into ecological risk assessment.

Sammendrag

Plantevernmidler er et viktig verktøy i dagens plantevernpraksis i jordbruket for å sikre gode avlinger. Miljørisikoen knyttet til det enkelte plantevernmiddel vurderes nøye før det godkjennes for bruk, men langvarig overvåking er nødvendig for å avdekke de faktiske miljøkonsentrasjoner og - effekter etter forskriftsmessig bruk av plantevernmidler. Sveriges nasjonale miljøovervåkingsprogram for plantevernmidler startet i 2002. Hovedmålet med programmet er å følge langtidstrender i påvirkningen av jordbrukets plantevernmiddelbruk på kvaliteten av overflate- og grunnvann, samt å bestemme miljøkonsentrasjonene av plantevernmidler i sediment, luft og nedbør. Formålet med denne evalueringen var å vurdere styrker og svakheter ved overvåkingsprogrammet, samt behov for endringer i den praktiske gjennomføringen, rapporteringsprosedyrer og målsetningen med programmet. Denne evalueringen vurderer også behovene hos de aktuelle sluttbrukergruppene for programmet som inkluderer svensk landbruks- og miljøforvaltning, rådgivningstjenesten i landbruket, bønder og bondeorganisasjoner mv.

Sammendrag

In Northern Europe, future changes in land-use and weather patterns are expected to result in increased precipitation and temperature this may cause an increase in plant disease and insect pests. In addition, predicted population increase will change the production demands and in turn alter agricultural practices such as crop types and with that the use pattern of pesticides. Considering these variabilities and magnitudes of pesticide exposure to the aquatic environment still needs to be accounted for better in current probabilistic risk assessment. In order to improve ecological risk assessment, this study explores an alternative approach to probabilistic risk assessment using a Bayesian Network, as these can serve as meta-models that link selected input and output variables from other models and information sources. The developed model integrates variability in both exposure and effects in the calculation of risk estimate. We focus on environmental risk of pesticides in two Norwegian case study region representatives of northern Europe. Using pesticide fate and transport models (e.g. WISPE), environmental factors such as soil and site parameters together with chemical properties and climate scenarios (current and predicted) are linked to the exposure of a pesticide in the selected study area. In the long term, the use of tools based on Bayesian Network models will allow for a more refined assessment and targeted management of ecological risks by industry and policy makers.