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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2016

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The purpose of this study is to increase the basic understanding of outbreak dynamics in order to improve the management of bark beetle outbreaks. The spruce bark beetle Ips typographus is a major disturbance agent of European forests and is the continent’s most economically and environmentally damaging bark beetle. Outbreaks of the spruce bark beetle are often triggered by large windfall episodes, and we have utilized a unique opportunity to study a Slovakian outbreak where little salvage logging was performed in some areas after a 2.5 million m3 storm-felling in 2004. Our analyses focused on the first five years after the windfall, and we used a combination of empirical data and simulation models to understand the spatial patterns of beetle-killed forest patches developing during the outbreak. The univoltine beetle population used an increasing proportion of the windfelled trees during the two first seasons after the storm, but from the third season onwards our comparisons of inter-patch distance distributions indicated a transition from beetle production largely in windfall areas to a self-sustaining outbreak with infestation patches developing independently of the windthrows. The size of new infestation patches formed after this transition was modeled as a function of beetle pressure, estimated by the proportion of a circle area surrounding new patches that was covered by infestation patches the previous year. Our model results of patch size distribution did not correspond well with the empirical data if patch formation was modeled as a pure dispersal–diffusion process. However, beetle aggregation on individual trees appears to be important for patch development, since good correspondence with empirical data was found when beetle aggregation was incorporated in the modeled dispersal process. The strength of correspondence between the beetle aggregation model and the empirical data varied with the density of aggregation trees in the modeled landscape, and reached a maximum of 83% for a density of three aggregation trees per infestation patch. Our results suggest that efficient removal of windfelled trees up until the start of the second summer after a major windfall is important to avoid a transition into a patch-driven bark beetle outbreak that is very difficult to manage. Our results also indicate that the outcome of a patch-driven outbreak is difficult to predict, since the development of new infestation patches is not a simple function of beetle pressure but is also affected by beetle behavior and local forest conditions.

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Nonylphenols (NP) are a group of alkylphenols, formed upon degradation of nonylphenol ethoxylates such as nonylphenol monoethoxylate (NP1EO) or nonylphenol diethoxylate (NP2EO), which have been broadly used as non-ionic surfactants. Both NP and their ethoxylates are often present in the sewage, despite being banned and substituted by less toxic alcohol ethoxylates in many countries. There is a number of degradation studies of nonylphenol in the soil environment, but there is a lack of understanding on how plants and soil organisms such as earthworms can affect the degradation. In our study, we investigated the degradation of 4-nonylphenol (4-NP) in a mineral field soil in the presence of barley (Hordeum vulgare) and earthworms (Aporrectodea caliginosa). Soil was spiked with 4-NP at a concentration of 12.5 mg kg-1 d.w. soil. Results showed that the degradation of 4-NP in soil was rapid during the 28 days after spiking, with remaining concentration of 0.397 mg kg-1 d.w. soil on day 28. Degradation was much slower between days 28 and 120, with a remaining concentration of 0.214 mg kg-1 d.w. soil on day 120. No significant difference in the degradation of 4-NP in the presence of either plants or worms was observed, but sampling after 28 days of exposure revealed transfer of 4-NP to worms (worm tissue concentration = 0.79 μg g-1), which increased with time (1.66 μg g-1 after 120 d). The calculated transfer factor after 28 (TF28) and 120 days (TF120) was 0.07 and 0.13 respectively. No toxicity or accumulation in plants was observed at the concentration tested herein. Concentration of 4-NP in the rhizosphere was not statistically different from that in the bulk soil.

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Denne rapporten sammenstiller erfaringer fra et pilotprosjekt der hovedmålet har vært å gjennomføre en uttesting av metodikk for innhenting av arealrepresentativ statistikk for naturtyper etter NiN-systemet, med utgangspunkt i Landsskogtakseringen. Erfaringene skal danne grunnlag for vurdering av mulighetene for en fullskala landsdekkende NiN-registrering i skog og på tresatte arealer. Delmål har vært å avklare 1) hvilke variabler i Landsskogtakseringen som kan anvendes i sin nåværende form ved registreringer etter NiN 2.0, og 2) hvilke nye registreringsvariabler, eventuelt endringer av eksisterende, som er nødvendig. To alternative registreringsopplegg ble utarbeidet og er testet ut av lagledere i Landsskogtakseringen. Disse har taksert over 350 landsskogflater i ulike landsdeler etter begge opplegg, og et utvalg av flatene (46) er også taksert av en biolog fra Naturhistorisk museum ved Universitetet i Oslo, uavhengig av Landsskogtakseringens lagledere. Resultatene viser at en ved å anvende et opplegg basert på en kombinasjon av eksisterende variabler i Landsskogtakseringen og ved å inkludere noen nye fra NiN i tillegg, vil kunne framskaffe et datagrunnlag for å beregne fordelingen av naturtyper i skogsmark og på andre tresatte arealer etter et femårig omdrev. Erfaringene fra prosjektet viser imidlertid også betydningen av å få på plass et godt opplegg for NiN-opplæring og kalibrering av laglederne. I rapporten gis det et anslag over ressursbehov knyttet til en fullskala implementering av NiN- registreringer inkludert kostnader knyttet til kursing og opplæring av inventørene. Før en kan sette i gang med registreringer over et femårig omdrev, er det behov for å avklare med oppdragsgiver hvilket omfang registreringene skal ha og hvordan registreringsopplegget for noen av variablene fra beskrivelsessystemet i NiN skal utformes.

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While tetraploid plants of red clover are taller, have thicker stems, and have broader leaves that altogether result in a higher forage yield compared to diploids, they generally have substantially lower seed yields than diploid plants. Tetraploid red clover can be induced chemically by colchicine or nitrous oxide (N2O) and sexually by union of unreduced gametes. The average seed yield of tetraploid red clover in Norway is 60% of the diploid yield, while in Sweden it is 75%. One objective of this paper was to examine whether there is a difference in seed yield among chromosome doubled tetraploids and crossed tetraploids. A second objective was to investigate differences in seed yield and seed yield components in Norwegian and Swedish tetraploid populations. The third objective was to study which yield component most correlates with the seed yield per hectare. Seed production experiments were established at Landvik and Bjørke in Norway and Svalöv and Lännäs in Sweden. Populations made by crossings of tetraploids gave significantly greater yield (p < 0.001) compared to populations that were made by chromosome doubling. On average, Norwegian and Swedish varieties had equal yields in both experimental years. Norwegian and Swedish varieties differed mostly in earliness traits. Swedish populations began flowering on average 4 d earlier than Norwegian populations. Genotypic correlations showed that seed yield per flower head was the component with the highest correlation (r = 0.956 and r = 0.977) with yield per hectare in both experimental fields. Results from the second experimental year indicate a trend towards improved seed yield after several cycles of recurrent selection for higher seed yield per flower head.

Sammendrag

The plant pathogenic fungus Fusarium langsethiae produces the highly potent mycotoxins HT-2 and T-2. Since these toxins are frequently detected at high levels in oat grain lots, they pose a considerable risk for food and feed safety in Norway, as well as in other north European countries. To reduce the risk of HT-2/T- 2-contaminated grain lots to enter the food and feed chain, it is important to identify factors that influence F. langsethiae infection and mycotoxin development in oats. However, the epidemiology of F. langsethiae is unclear. A three-year survey was performed to reveal more of the life cycle of F. langsethiae and its interactions with oats, other Fusarium species, as well as insects, mites and weeds. We searched for inoculum sources by quantifying the amount of F. langsethiae DNA in weeds, crop residues, and soil, sampled from a predetermined selection of oat-fields. To be able to define the onset of infection, we analysed the amount of F. langsethiae DNA in oat plant material sampled at selected growth stages (between booting and maturation), as well as the amount of F. langsethiae DNA and HT-2 and T-2 toxins in the mature grain. We also studied the presence of possible insect- and mite vectors sampled at the selected growth stages using Berlese funnel traps. All the different types of materials were also analysed for the presence F. graminearum DNA, the most important deoxynivalenol producer observed in Norwegian cereals, and which presence has shown a striking lack of correlation with the presence F. langsethiae in oat. Preliminary results show that F. langsethiae DNA may occur in the oat plant before heading and flowering. Some F. langsethiae DNA was observed in crop residues and weeds, though at relatively low levels. More results from this work will be presented at the meeting.

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Food production contributes considerably to global greenhouse gas (GHG) emissions. Animal products – particularly meat from ruminants – generally have higher GHG emissions than plant products. Over the last few decades the global per capita consumption of animal products has increased. This has a negative impact on climate change, land and water availability, and human health. We are faced with the two-fold challenge of reducing GHG emissions while still producing enough food for our growing population. Part of the solution could be for consumers to change towards a more sustainable diet. In this paper we take Norway as a case study for estimating optimal taxes and subsidies on different food items which can change consumption patterns in order to reduce the GHG emissions derived from the average Norwegian diet. In the estimate we ensure that the average calorie intake with the new diet remains the same as with the current diet, and factor in other health considerations. Our findings suggest that limited but useful emission reduction targets can be set with only a few changes in diets. The methodology presented in this paper may be used to estimate optimal climate taxes and subsidies under different emission, quantities, taxes, subsidies, and health constraints.

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The European plum cultivar 'Opal', widely grown in Scandinavia, frequently initiates too many flowers and set too many fruits. If excess fruitlets remain on the trees until harvest, the crop consists of small, unmarketable fruits of low fruit quality and return bloom will be reduced. The current study investigated effects of different crop loads established at blossom and fruitlet stages on yield, fruit quality and return bloom. For two seasons starting in 2008 on mature 'Opal'/'St. Julien A' trees, two crop loads 50 and 25% flowers reduced were established at full bloom and at 10-12 mm fruitlet size and compared with an un-thinned control treatment. Treatments were applied on single whole trees in a randomized complete block design with six replications. Spindle trees spaced at 3.0×5.0 m were selected for uniformity in growth habit, flowering intensity and trunk cross sectional area. Final fruit set varied from 63% on the control trees to 18% when thinned at bloom. Yield was negatively correlated with the fruit set response. Thinning at the fruitlet stage resulted in smaller fruits at the same crop level compared to flower thinning. Fruit quality parameters characterized by bright yellow skin background colour, red surface colour and the concentrations of soluble solids increased significantly as the crop load was reduced. Other fruit quality parameters like percentage acidity were not significantly different and did not show a clear response to the thinning. Return bloom was promoted most when trees were thinned at bloom the year before.

Sammendrag

Important losses in strawberry production are often caused by the oomycete Phytophthora cactorum, the causal agent of crown rot. However, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. To begin to rectify this situation, a PCR-based approach (NBS profiling) was used to isolate strawberry resistance gene analogs (RGAs) with altered expression in response to P. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h post-infection). Twenty-three distinct RGA fragments of the NB-LRR type were identified from a resistance genotype (Bukammen) of the wild species Fragaria vesca. The gene transcriptional profiles after infection showed that the response of most RGAs was quicker and stronger in the resistance genotype (Bukammen) than in the susceptible one (FDP821) during the early infection stage. The transcriptional patterns of one RGA (RGA109) were further monitored and compared during the P. cactorum infection of two pairs of resistant and susceptible genotype combinations (Bukammen/FDP821 and FDR1218/1603). The 5′ end sequence was cloned, and its putative protein was characteristic of NBS-LRR R protein. Our results yielded a first insight into the strawberry RGAs responding to P. cactorum infection at molecular level.

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The response of three-year-old 'Summerred' trees to crop load regulation by the chemical thinning agent Brevis® was tested in comparison with different levels of hand thinning. Brevis® was applied once or twice at a dosage of 1.1 and 2.2 kg ha-1 when fruitlets were approximately 15 and 19 mm in diameter at the time of the first and second application, respectively. All Brevis® applications significantly reduced the number of fruits tree-1. Both the single application of 2.2 kg ha-1 and the two-fold application of 1.1 kg ha-1 thinned the tree to the target crop load level of 30 apples tree-1. The degree of thinning by the single application of 1.1 kg ha-1 Brevis® did not significantly increase fruit weight and resulted in a very strong inhibition of return bloom similar to that observed in the untreated controls and trees hand thinned to 60 fruits tree-1. Return bloom was positively related to the level of thinning obtained. At the target fruit load of about 30 apples tree-1, return bloom was around 40% of the bloom in the year of the thinning trial. A further thinning to circa 15 fruits tree-1 increased return bloom to approximately 60%. In conclusion, 'Summerred' trees planted as two-year-old feathered trees should not bear more than 20 to 25 fruits tree-1 in their 3rd leaf in a northern climate to avoid the onset of biennial bearing and to ensure regular annual yields. Only then sufficient numbers of flower buds will develop to have the chance to obtain a good crop in the next year.