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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2020

Sammendrag

Phosphorus retention and bank erosion was investigated in two types of buffer zones in cereal fields in Norway: zones used for grass production and zones with natural vegetation. Farmers’ views on the two types of buffer zones were collected through questionnaires and indepth interviews. Our results indicate that the grassed buffer zones had higher levels of plant-available phosphorus and lower infiltration rates than the natural ones. Bank erosion was higher in zones with grass production than those with trees. Interviews with farmers revealed diverging opinions on the zones. Most farmers were sceptical to natural vegetation with trees, whereas farmers who had already planted trees in the riparian zones were generally satisfied. Buffer zones can have many different functions, and we conclude that a holistic approach is needed when assessing the usefulness of this measure, taking into account water quality, biodiversity and the production of food, fodder and biomass.

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Sammendrag

During surveys of insect-associated mycobiomes in Norway, Poland, and Russia, isolates with affinity to Graphilbum (Ophiostomatales, Ascomycota) were recovered. In this study, eight known Graphilbum species as well as the newly collected isolates were compared based on morphology and DNA sequence data for four gene regions. The results revealed seven new species, described here as G. acuminatum, G. carpaticum, G. curvidentis, G. furuicola, G. gorcense, G. interstitiale, and G. sexdentatum. In addition to these species, G. crescericum and G. sparsum were commonly found in Norway. All new species were recovered from conifers in association with bark beetles, cerambycid beetles, and weevils and were morphologically similar, predominantly with pesotum-like asexual morphs. Where sexual morphs were present, these were small ascomata with short necks and rodshaped ascospores having hyaline sheaths. The results suggest that Graphilbum species are common members of the Ophiostomatales in conifer ecosystems.

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Sammendrag

The extraction of Rhodiola rosea rhizomes using natural deep eutectic solvent (NADES) consisting of lactic acid, glucose, fructose, and water was investigated. A two-level Plackett–Burman design with five variables, followed by the steepest ascent method, was undertaken to determine the optimal extraction conditions. Among the five parameters tested, particle size, extraction modulus, and water content were found to have the highest impact on the extrability of phenyletanes and phenylpropanoids. The concentration of active compounds was analyzed by HPLC. The predicted results showed that the extraction yield of the total phenyletanes and phenylpropanoids (25.62 mg/g) could be obtained under the following conditions: extraction time of 154 min, extraction temperature of 22 °C, extraction modulus of 40, molar water content of 5:1:11 (L-lactic acid:fructose:water, mol/mol), and a particle size of rhizomes of 0.5–1 mm. These predicted values were further verified by validation experiments in predicted conditions. The experimental yields of salidroside, tyrosol, rosavin, rosin, cinnamyl alcohol and total markers (sum of phenyletanes and phenylpropanoids in mg/g) were 11.90 ± 0.02, 0.36 ± 0.02, 12.23 ± 0.21, 1.41 ± 0.01, 0.20 ± 0.01, and 26.10 ± 0.27 mg/g, respectively, which corresponded well with the predicted values from the models.

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Sammendrag

Sweet potato (Ipomoea batatas L. Lam) has become one of the staple crops in Africa in the last 20 years. In Ethiopia, sweet potato is the second most widely grown root crop and is the first regarding the production per hectare. Thus, there is a great demand of planting material throughout the country. Currently, planting material is usually obtained from own previous season harvest, local markets or from the neighboring fields since no certified clean planting material production scheme has been established in Ethiopia yet. Unfortunately, this practice has contributed to the spread of viral diseases throughout the country. Elimination of viruses from infected plants is a tedious job, which requires efficient methods to eliminate the virus and also to verify that the plants are indeed virus-free. In the case of sweet potato, it was observed that heat treatment, combined with meristem tip culture is an efficient method for virus elimination. Previous findings indicate that reverse transcription (RT) PCR is more efficient than ELISA to verify the efficiency of virus elimination. In this study, the use of next generation sequencing (NGS) was explored as a verification method and compared with RT-PCR. The results show that NGS seems to be more efficient than RT-PCR, although also prone to inconclusive results.

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The triploid pear cultivar ‘Ingeborg’ is currently the main commercial pear cultivar grown in Norway. However, fruit set and subsequent yields of this cultivar have proven to be variable and overall rather low. In order to promote the fruit set, different bioregulators were applied during and after bloom and compared with an untreated control. Investigations were done during the period 2017-2018, at NIBIO Ullensvang, western Norway. Different dosages of both gibberellins (GA3, trade name GIBB 3, 10% active ingredient (a.i.) and GA4/7, trade name Novagib®, 1% a.i.) were applied at full bloom and at petal fall. Additional applications of the growth retardant prohexadioneCa (trade name Regalis®, 10% a.i.) were applied twice, when bourse shoots had 3-5 leaves and after one month later. Ethephon (Cerone (480 g a.i. L-1)) was applied three times starting about 7 days after petal fall with ca. 7- to 10-day intervals. All gibberellin applications significantly increased fruit set compared to the untreated control. One single application with GA3 (3 g ha-1) almost tripled the fruit number per 100 flower clusters when compared with the control (136 and 46, respectively). The yield response was similar (16.8 to 9.6 kg tree-1, respectively). Similar results occurred with one application of GA4/7 (12 g ha-1) with the same crop load level, and the fruit weights were similar to the control (130 g). Prohexadione-Ca treatments significantly reduced shoot growth of the pear trees. Two treatments with 125 g ha-1 or one treatment of 250 g ha-1 reduced the growth by ~35% but had no significant effect on fruit set and yield. The multiple ethephon applications (275 mL ha-1 in total) had no effect on both set and shoot growth, and return bloom compared to the untreated or gibberellin treated trees.

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Sammendrag

Risk models for decisions on fungicide use based on weather data, disease monitoring, and control thresholds are used as important elements in a sustainable cropping system. The need for control of leaf blotch diseases in wheat (caused by Zymoseptoria tritici, Parastagonospora nodorum and Pyrenophora tritici-repentis) vary significantly across years and locations. Disease development is mainly driven by humidity events during stem elongation and heading. Two risk models were tested in field trials in order to identify situations favourable for the development of leaf blotch diseases in Lithuania, Norway, Sweden, Finland and Denmark. The Crop Protection Online (CPO) model uses days with precipitation (>1 mm), while the humidity model (HM) uses 20 continuous hours with relative humidity (RH) ≥ 85% as criteria for the need of a fungicide application. Forty-seven field trials were carried out during two seasons to validate these two risk-models against reference fungicide treatments. The season 2018 was dry and 2019 had an average precipitation profile. The two risk models with few exceptions provided acceptable disease control. In 2018, very few treatments were recommended by the models, saving 85–98% of treatments compared to the reference treatments, while in the wetter season 2019, 31% fewer applications were recommended. Based on specific criteria including fungicide input and net yield responses the models gave correct recommendations in 95% of the trials in 2018 and in 54–58% of the trials in 2019 compared with reference treatments dominated by 2–3 sprays. In comparison with single spray references, the models gave correct recommendations in 54–69% of the situations.