Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2021
Authors
Yanli Man Marianne Stenrød Chi Wu Marit Almvik Roger Holten Jihong Liu Clarke Shankui Yuan Xiaohu Wu Jun Xu Fengshou Dong Yongquan Zheng Xingang LiuAbstract
Difenoconazole is a widely used triazole fungicide that has been frequently detected in the environment, but comprehensive study about its environmental fate and toxicity of potential transformation products (TPs) is still lacking. Here, laboratory experiments were conducted to investigate the degradation kinetics, pathways, and toxicity of transformation products of difenoconazole. 12, 4 and 4 TPs generated by photolysis, hydrolysis and soil degradation were identified via UHPLC-QTOF/MS and the UNIFI software. Four intermediates TP295, TP295A, TP354A and TP387A reported for the first time were confirmed by purchase or synthesis of their standards, and they were further quantified using UHPLC-MS/MS in all tested samples. The main transformation reactions observed for difenoconazole were oxidation, dechlorination and hydroxylation in the environment. ECOSAR prediction and laboratory tests showed that the acute toxicities of four novel TPs on Brachydanio rerio, Daphnia magna and Selenastrum capricornutum are substantially lower than that of difenoconazole, while all the TPs except for TP277C were predicted chronically very toxic to fish, which may pose a potential threat to aquatic ecosystems. The results are important for elucidating the environmental fate of difenoconazole and assessing the environmental risks, and further provide guidance for scientific and reasonable use.
Abstract
Aquaculture has undergone rapid development in the past decades. It provides a large part of high-quality protein food for humans, and thus, a sustainable aquaculture industry is of great importance for the worldwide food supply and economy. Along with the quick expansion of aquaculture, the high fish densities employed in fish farming increase the risks of outbreaks of a variety of aquatic diseases. Such diseases not only cause huge economic losses, but also lead to ecological hazards in terms of pathogen spread to marine ecosystems causing infection of wild fish and polluting the environment. Thus, fish health is essential for the aquaculture industry to be environmentally sustainable and a prerequisite for intensive aquaculture production globally. The wide use of antibiotics and drug residues has caused intensive pollution along with risks for food safety and increasing antimicrobial resistance. Vaccination is the most effective and environmentally friendly approach to battle infectious diseases in aquaculture with minimal ecological impact and is applicable to most species of farmed fish. However, there are only 34 fish vaccines commercially available globally to date, showing the urgent need for further development of fish vaccines to manage fish health and ensure food safety. Plant genetic engineering has been utilized to produce genetically modified crops with desirable characteristics and has also been used for vaccine production, with several advantages including cost-effectiveness, safety when compared with live virus vaccines, and plants being capable of carrying out posttranslational modifications that are similar to naturally occurring systems. So far, plant-derived vaccines, antibodies, and therapeutic proteins have been produced for human and animal health. However, the development of plant-made vaccines for animals, especially fish, is still lagging behind the development of human vaccines. The present review summarizes the development of fish vaccines currently utilized and the suitability of the plant-production platform for fish vaccine and then addresses considerations regarding fish vaccine production in plants. Developing fish vaccines by way of plant biotechnology are significant for the aquaculture industry, fish health management, food safety, and human health.
Abstract
Forest harvest residue is a low-competitive biomass feedstock that is usually left to decay on site after forestry operations. Its removal and pyrolytic conversion to biochar is seen as an opportunity to reduce terrestrial CO2 emissions and mitigate climate change. The mitigation effect of biochar is, however, ultimately dependent on the availability of the biomass feedstock, thus CO2 removal of biochar needs to be assessed in relation to the capacity to supply biochar systems with biomass feedstocks over prolonged time scales, relevant for climate mitigation. In the present study we used an assembly of empirical models to forecast the effects of harvest residue removal on soil C storage and the technical capacity of biochar to mitigate national-scale emissions over the century, using Norway as a case study for boreal conditions. We estimate the mitigation potential to vary between 0.41 and 0.78 Tg CO2 equivalents yr−1, of which 79% could be attributed to increased soil C stock, and 21% to the coproduction of bioenergy. These values correspond to 9–17% of the emissions of the Norwegian agricultural sector and to 0.8–1.5% of the total national emission. This illustrates that deployment of biochar from forest harvest residues in countries with a large forestry sector, relative to economy and population size, is likely to have a relatively small contribution to national emission reduction targets but may have a large effect on agricultural emission and commitments. Strategies for biochar deployment need to consider that biochar's mitigation effect is limited by the feedstock supply which needs to be critically assessed.
Abstract
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Authors
Wiktoria Kaczmarek-Derda Zahra Bitarafan Belachew Asalf Tadesse Marit Skuterud Vennatrø Erik J. Joner Tobias Glemming Hans Kristian WestrumAbstract
Det er ikke registrert sammendrag
Authors
Hang Su Andre van Eerde Hege Særvold Steen Inger Heldal Sissel Haugslien Irene Ørpetveit Stefanie Caroline Wüstner Makoto Inami Marie Løvoll Espen Rimstad Jihong Liu ClarkeAbstract
Cardiomyopathy syndrome (CMS) is a severe cardiac disease occurring in the grow-out sea phase of farmed Atlantic salmon with approximately 100 outbreaks annually in Norway. Piscine myocarditis virus (PMCV) is believed to be the causative agent of CMS. There is no vaccine available to control CMS, partially because PMCV withstands propagation in known cell cultures. In the present study, we selected the putative capsid protein of PMCV as the candidate antigen for immunization experiments and produced it in the plant Nicotiana benthamiana by transient expression. The recombinant PMCV antigen formed virus-like particles (VLPs). To evaluate the efficacy of the plant made VLP vaccine, a PMCV infection model was established. In an experimental salmon vaccination trial, the VLP vaccine triggered innate immunity, and indicative but not significant inhibition of viral replication in heart, spleen and kidney tissues was observed. Similarly, a reduction of inflammatory lesions in cardiomyocytes and subendocardial infiltration by mononuclear leukocytes were observed. Therefore, there was no difference in efficacy or immune response observed post the plant made PMCV VLP antigen vaccination. Taken together, this study has demonstrated that plant made VLP antigens should be investigated further as a possible platform for the development of PMCV antigens for a CMS vaccine.
Authors
Anna Landrø Hjelt Siri Voll Dombu Ivar Pettersen Mathias Bjugan Anne Falk Øgaard Marianne Bechmann Helge BonesmoAbstract
Denne utredningen omfatter sentrale tema relatert til foreslåtte endringer i gjødselregelverket og oppdaterer og supplerer kunnskapsgrunnlaget for de endringene som foretas. En stor del av utredningen omhandler konsekvenser av skjerpede krav til spredeareal/tillatt mengde fosfor, herunder omfanget av overskudd av gjødsel gitt nye krav, nytte og kostnader ved ulik håndtering, og effektene av geografisk differensierte krav. Det gjøres også en komparativ analyse av hvordan utfordringer med gjødsel håndteres i EU og i land som kan regnes å være sammenliknbare med hensyn til gjødselproblematikk. Utredningen omfatter også vurderinger knyttet til miljøeffekter av foreslåtte innstramminger av spredetidspunkt…..
Authors
Siv Kristin Sællmann Iselin Elise Fjeld Ellen Johanne Svalheim Trygve S. Aamlid Olaug Bollestad Maren Hersleth HolsenAbstract
Forskerne kunne tjuedoblet produksjonen av norske markblomster, men mangler fortsatt midler. I fem år har de ventet på en lovet plan mot insektdød fra regjeringen.
Abstract
Det er ikke registrert sammendrag
Abstract
Det er ikke registrert sammendrag