Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2018
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Fusarium head blight and seedling blight, both caused by Fusarium spp. and Microdochium spp., and glume blotch caused by Parastagonospora nodorum, are important diseases in wheat. In Norway, wheat seed lots are routinely analysed for infestation by these pathogens using traditional methods (plating grain on PDA, recording presence or absence of fungal colonies). This method is time consuming, require knowledge within fungal morphology, and do not facilitate identification to species in all cases. Molecular methods such as quantitative PCR (qPCR) could allow detection and quantification of fungal DNA at the species level in a relatively time effective way, particularly since the method allows for automation in different steps such as DNA extraction and pipetting. Whether the latter method is suitable within seed health evaluations will depend on the relationship between the amount of DNA of the different fungal species and field performance, and the purpose of the test (evaluation of planting value, need for seed treatment, survey of fungal species, quality of grain for consumption etc). To compare the two different methods, about 150 spring wheat seed lots from the years 2016-2017 (including two cultivars) were selected for the analysis of different fungi using species-specific qPCR and compared with the results from routine testing on PDA. In the 2016 material (81 samples), a mean seed infestation rate of 26% was observed for Microdochium spp. in the PDA test. The level of Fusarium was lower (mean infestation rate of 5%). A strong relationship was observed between the percentage of seeds infested by Microdochium and the level of Microdochium DNA (sum of DNA from Microdochium majus and Microdochium nivale) quantified by qPCR (R2 of 0.76, p<0.01). The relationship between Fusarium infested seeds and the level of Fusarium DNA (sum of DNA from three species) was moderate (R2 of 0.33, p<0.01). The samples were also analysed for the presence of P. nodorum. Compared to Fusarium and Microdochium, P. nodorum was present at an intermediate level (mean infestation rate of 12%). The relationship between the two different methods was weaker for this fungus (R2 of 0.21, p<0.01) than for Fusarium and Microdochium. The relationship between germination capacity and rating of the three groups of fungi by either method was studied. Preliminary results suggest that of the three fungi, Microdochium was associated with germination capacity in the 2016 material, and that the Microdochium infestation rate on PDA was slightly better correlated to germination capacity than the level of Microdochium DNA. Further results will be presented at the conference, including the association between the relative DNA content of the different Microdochium and Fusarium species and seed germination.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Yupeng Zhang Guangxun Fan Tuomas Toivainen Igor A. Yakovlev Timo Hytönen Paal Krokene Paul Eivind Grini Carl Gunnar FossdalSammendrag
Climate change is one of the greatest challenges for the biosphere. As sessile organisms, plants must adapt quickly to keep pace with the rapidly changing climatic conditions. Epigenetic memory is one mechanism which would provide sufficient plasticity under rapid climate change and enable long-lived organisms to survive long enough to adapt by classical genetic selection. In Norway spruce, the timing of bud burst and bud set are regulated by an epigenetic memory established by the temperature sum endured during embryogenesis. The resulting epitypes display a life-long shift in seasonal timing of the bud phenology, a trait previously presumed to be under strict classical selection and highly heritable. However, Norway spruce is a difficult plant to study because it has a very long generation time and an extensive genome size. We therefore seek to find a suitable perennial model plant to study the phenomenon of epigenetic climatic memory. Woodland strawberry (Fragaria vesca) may be an ideal model to research the role of epigenetic memory on plant phenology. Fragaria vesca is a perennial plant with a small well-characterized genome, a short sexual reproduction cycle and can also propagate asexually trough clonal daughter plants formed by stolons. We will explore whether the temperature sum experienced during sexual and asexual reproduction impact on the phenology of Fragaria vesca and use this as a model to decipher the molecular mechanism underlying epigenetic memory in plants.
Forfattere
Kirsten Tørresen Lars Olav Brandsæter Jan Netland Therese With Berge Björn Ringselle Einar StrandSammendrag
Glyfosat er det mest brukte plantevernmidlet i Norge og på verdensbasis. Det har i de siste årene vært diskutert om dette ugrasmiddelet skulle få fornyet godkjenning. I 2017 ble glyfosat godkjent for fem nye år til 2022. Dersom glyfosat fases ut er en redd det vil få store negative konsekvenser for jordbruk og matproduksjon. Glyfosat brukes til å bekjempe ugras og andre uønska planter på dyrka og udyrka arealer. I jordbruket er glyfosat spesielt viktig for å begrense ugrasets avlingsreduserende effekt. I norsk jordbruk brukes glyfosat hovedsakelig til å bekjempe ugrasarten kveke i korndominerte omløp og ved fornying av grasmark. Ved redusert jordarbeiding er glyfosat viktig for bekjemping av flerårige og andre overvintrende ugras. Vi har i denne rapporten kartlagt kunnskap relevante for norske forhold på hvilke ikke-kjemiske og kjemiske alternativer til glyfosat som en har/kan få i framtida i korn og grasmark slik at matproduksjonen kan opprettholdes. Mekaniske tiltak som pløying og ulike former for jordarbeiding i stubben mot kveke og andre ugras, og radrensing i korn mot ugras generelt er viktige alternativer til glyfosat. Det er også noen nye redskapstyper (rotskjærere) som virker lovende i bekjemping av ugras. Videre så vil en god jord- og plantekultur med et godt vekstskifte bidra til å holde ugraset under kontroll. Per i dag fins det noen få kjemiske alternativer mot kveke i hvete, rughvete og rug og mot tofrøblada rotugras i korn. Det er andre kjemiske alternativer som kan undersøkes mer for bruk i stubbåker/til brakking av grasmark som for eksempel ulike organiske syrer og grasugrasmidler for tofrøblada kulturer........
Sammendrag
Pærevisnesjuke regnes som en av de mest alvorlige sjukdommene på pære. Pærevisnesjuke forårsakes av fytoplasma, små veggløse bakterier som lever i silvevet i planter Dette er en karanteneskadegjører i Norge som ble påvist her i landet for første gang i 2015. I 2016 og 2017 ble det gjenomført et OK-program for denne skadegjøreren. Det ble totalt analysert 853 prøver i OK-programmet i 2016. Det ble totalt påvist pærevisnesjuke i 72 av prøvene. Det ble påvist pærevisnesjuke i 10 av 44 undersøkte frukthager. I 2017 ble det undersøkt 260 prøver fra 14 lokaliteter i fire fylker. Det påvist pærevisnesjuke i 13 prøver fra to lokaliteter.I 2017 ble også innsamlede sugere testet for pærevisnesjuke. Av i alt 304 individer fra 6 lokaliteter var 21 individer fra to lokaliteter infisert.
Forfattere
Maria Hayes Leen Bastiaens Luisa Gouveia Spyros Gkelis Hanne Skomedal Kari Skjånes Patrick Murray Marco García-Vaquero Muge Isleten Hosoglu John Dodd Despoina Konstantinou Ivo Safarik Graziella Chini Zittelli Vytas Rimkus Victόria del Pino Koenraad Muylaert Christine Edwards Morten Laake Joana Gabriela Laranjeira da Silva Hugo Pereira Joana AbelhoSammendrag
Det er ikke registrert sammendrag
Sammendrag
Sclerotinia stem rot (SSR) is the most important disease of oilseed Brassica crops in Norway. Fungicide applications should be aligned with the actual need for control, but the SSR prediction models used lack accuracy. We have studied the importance of precipitation, and the role of petal and leaf infection for SSR incidence by using data from Norwegian field and trap plant trials over several years. In the trials, SSR incidence ranged from 0 to 65%. Given an infection threshold of 25% SSR, regression and Receiver Operating Characteristics (ROC) analysis were used to evaluate different precipitation thresholds. The sum of precipitation two weeks before and during flowering appeared to be a poor predictor for SSR infection in our field and trap plant trials (P = 0.24, P = 0.11, respectively). Leaves from three levels (leaf one, three, five), and petals were collected at three to four different times during flowering from nine field sites over two years and tested for SSR infection with real-time PCR. Percentage total leaf and petal infection explained 57 and 45% of variation in SSR incidence, respectively. Examining the different leaves and petals separately, infection of leaf three sampled at full flowering showed the highest explanation of variation in later SSR incidence (R2 = 65%, P < 0.001). ROC analysis showed that given an infection threshold of 45%, both petal and leaf infection recommended spraying when spraying was actually needed. Combining information on petal and leaf infection during flowering with relevant microclimate factors in the canopy, instead of the sum of precipitation might improve prediction accuracy for SSR.
Sammendrag
NIBIO BOOK 4(4):30-32
Sammendrag
Phytophthora cryptogea, P. gonapodyides, P. lacustris, P. megasperma, P. plurivora, P. taxon paludosa and an unknown Phytophthora species were isolated from waterways and soil samples in Christmas tree fields in southern Sweden. In addition, P. megasperma was isolated from a diseased Norway spruce (Picea abies) plant from one of the fields in Svalöv. Inoculation tests were sequentially carried out with one isolate from each of the three species P. cryptogea, P. megasperma, and P. plurivora, all known pathogens on conifers. The same three isolates were used to study a few morphological features to confirm the identification, and temperature-growth relationships were carried out to see how well the organisms fit into Swedish climatic conditions. Seedlings of Norway spruce and Nordmann fir (Abies nordmanniana) were inoculated in the roots and the stems. None of the isolates caused extensive root rot under the experimental conditions, but all three species could be re-isolated from both Norway spruce and Nordmann fir. Phytophthora root rot is currently of minor concern for Christmas tree growers in Sweden. However, the Phytophthora isolations from soil and water indicate the presence of this damaging agent, which may lead to future problems.