Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Andre van Eerde Aniko Varnai John-Kristian Jameson Lisa Paruch Anders Moen Jan Haug Anonsen Piotr Chylenski Hege Særvold Steen Inger Heldal Ralph Bock Vincent Eijsink Jihong Liu ClarkeSammendrag
Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Wiktoria Kaczmarek-Derda Marit Helgheim Jan Netland Hugh Riley Kjell Wærnhus Samson Øpstad Liv Østrem Lars Olav BrandsæterSammendrag
The abundance of Juncus effusus (soft rush) and Juncus conglomeratus (compact rush) has increased in coastal grasslands in Norway over recent decades, and their spread has coincided with increased precipitation in the region. Especially in water‐saturated, peaty soils, it appears from field observations that productive grasses cannot compete effectively with such rapidly growing rush plants. In autumn–winters of 2012–2013 and 2013–2014, a four‐factor, randomised block greenhouse experiment was performed to investigate the effect of different soil moisture regimes and organic matter contents on competition between these rush species and smooth meadow‐grass (Poa pratensis). The rush species were grown in monoculture and in competition with the meadow‐grass, using the equivalent of full and half the recommended seed rate for the latter. After about three months, above‐ and below‐ground dry matter was measured. J. effusus had more vigorous growth, producing on average 23–40% greater biomass in both fractions than J. conglomeratus. The competitive ability of both rush species declined with decreasing soil moisture; at the lowest levels of soil moisture, growth reductions were up to 93% in J. conglomeratus and 74% in J. effusus. Increasing water level in peat–sand mixture decreased competivitiveness of meadow‐grass, while pure peat, when moist, completely impeded its below‐ground development. These results show that control of rush plants through management may only be achieved if basic soil limitations have been resolved.
Forfattere
Knut-Erik Tollefsen Karina Petersen Ana Catarina Almeida Thomas Backhaus Tania Gomes H.R. Norli Sven R. Odenmarck Hans Ragnar Norli You Song Raoul Wolf Li Xie Joachim Sturve Marianne StenrødSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Venche Talgø Inger Sundheim Fløistad Martin Pettersson May Bente Brurberg C. Lilleåsen H. R. Bye Ø. M Edvardsen Guro BrodalSammendrag
Det er ikke registrert sammendrag
Sammendrag
Sommeren 2018 vil bli husket som meget varm. Varmesummen var betydelig høyere enn normalen ved de fleste målestasjonene i NIBIOs landbruksmeteorologisk tjeneste (LMT). Felles for Sør-Norge var en sein vår, og varmt og tørt fra andre uka i mai til begynnelsen av august. I Trøndelag og nordover var det varmt i april og mai, mens juni ble kjølig og regnfull. Temperaturen var høyere enn normalt i nord resten av sommeren, med lite nedbør i juli.
Populærvitenskapelig – Været i vekstsesongen 2019
Halvard Hole, Berit Nordskog, Brita Linnestad, ...
Sammendrag
En varm april ga en tidlig start på vekstsesongen 2019, men kjøligere vær i mai og nattefrost ga skader i fruktblomstringa på Østlandet. Sommeren startet kjølig og våt i deler av landet, men fra slutten av juli var det godt over normal temperatur de fleste steder. Det var store variasjoner i nedbør, både lokalt og regionalt. Mens enkelte steder i nord og øst fikk store nedbørsmengder i juni, fikk deler av Sør- og Vestlandet mye nedbør i både juli, august og september.
Forfattere
Nina Johansen Nina Trandem Marta Bosque Fajardo Elisa Gauslå Marit Helgheim Jan Karstein HenriksenSammendrag
Det er nylig funnet nedsatt følsomhet og resistens mot enkelte plantevernmidler hos veksthusspinnmidd fra fem norske bær-produsenter. Dette kan bety at vi har et begynnende resistensproblem. For å unngå å få stor bekjempelsesproblemer er det viktig å bruke kjemiske midler minst mulig og på en måte som holder resistensen i sjakk.