Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2016
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Sammendrag
Microsatellite markers are one of the most valuable genetic marker because of high polymorphism, codominant, high reproducibility and relatively high abundance in the genome. Classical techniques to identify and to develop microsatellite markers are time-consuming and require cloning and library construction followed by Sanger sequencing. In the recent years Next Generation Sequencing (NGS) have been widely used to identify molecular markers for non-model organisms. To test the efficiency of NGS techniques in developing molecular markers, we have used double digest Restriction site Associated DNA Sequencing (ddRADseq) to identify microsatellites in Heracleum. Genomic DNA from three individuals digested with SbfI and NdeI followed by size selection and library construction and then DNA fragments were sequenced with Ion Torrent PGM. After trimming adaptors and evaluating the quality of reads, QDD software was used to screen reads with microsatellite motives containing two, three, four, five and six nucleotide repeats. Almost 2% of all sequences were consisted microsatellites repeats. Fifty four singleton and consensus sequences were bioanformatically confirmed and were checked for contamination and similarity with NCBI nucleotide database. Seventy percent of the sequences were represented by (AT)n, (AT)n, (GA)n and (AC)n motives. Twenty five primer pairs were selected to test for amplification and the results showed that most of the loci produced the expected size on Agarose gel. Our results show the high efficiency of ddRADseq in developing sufficient number of markers in a short time where the budget is also limited. Keywords: Microsatellites, Next Generation Sequencing, ddRADseq, Heracleum
Forfattere
Lise GrøvaSammendrag
Det er ikke registrert sammendrag
Forfattere
Lise GrøvaSammendrag
Presentasjon av KRUS-prosjektet på NIBIO samling.
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Forfattere
Lise GrøvaSammendrag
Det er ikke registrert sammendrag
Forfattere
Tor MykingSammendrag
Det er ikke registrert sammendrag
Sammendrag
Uncoated wood is increasingly used in building façades in Northern Europe. The benefits can be both low maintenance and low environmental impact. An even, grey color of the façade is often the intention, but in several cases, an uneven pattern of light, grey and dark/black areas is the result. The effect of weathering and the influencing factors have to be considered when designing and building uncoated and coated wooden façade to avoid unwanted weathering effects. Wood weathering is commonly described to be caused by: accumulation of extractives on the surface, photo-degradation of lignin, staining by dark colored mould fungi, checking, cracking and splitting due to moisture fluctuation and erosion. This paper reviews, analyzes and exemplifies weathering as a finishing system and design option for uncoated wood. Furthermore, it also discusses and exemplifies the main influencing factors and weathering effects on coated wood in facades.
Sammendrag
There is an increasing awareness of how the aesthetical performance of wood exposed outdoors changes over time and especially in the first few years after installation. Mould and blue stain fungi are biological agents that contribute to the weather grey colour on a wooden façade, and the blue stain fungi Aureobasidium pullulans is commonly identified as colonizer on coated and uncoated wood exposed outdoors. In this study 21 wood substrates (untreated, preservative treated and modified) were tested for their susceptibility to A. pullulans when incubated at three different temperatures (11, 16 and 22°C). Western red cedar and preservative treated wood had the lowest mould ratings at the end of the test period (84 days). Alder, ash, Norway spruce and Sitka spruce reached maximum rating already at day 28, and at day 84 also aspen, European larch, thermally modified pine, birch, acetylated pine and DMDHEU modified pine had reached maximum rating. Incubation temperature had a significant influence on the growth of A. pullulans throughout the test period for acetylated and DMDHEU modified samples – and generally the modified wood substrates were more sensitive to changes in temperature than the other tested substrates. Scots pine sapwood seemed to be less susceptible to A. pullulans in mono cultures, demonstrating low mould ratings throughout the test period. This contradicts to previous studies were Scots pine sapwood tended to have high susceptibility when using a mix of mould and blue stain fungi.
Forfattere
Lone Ross Thomas Kringlebotn Thiis Geir Isak Vestøl Ingunn Burud Solrun Karlsen Lie Olav Albert HøibøSammendrag
Use of uncoated wood in building façades has increased the last years, and therefore it is important to understand and control the aesthetical character and appearance of wood materials used outdoors. By predicting the aesthetical service life and the visual appearance of a wooden façade over time, we can accommodate some of the end-user expectations, perception, values and aesthetical preferences. The spatial variation of climate on a façade is large, and estimation of the weathering process and mould growth on outdoor exposed wood should be based on the actual surface climate rather than data from meteorological stations. A first version of a simulation model that aims to predict and visualize future aesthetical appearance of wooden façades has been developed. The model is based on existing mould growth models and on a new model for surface meteorological conditions that account for RH, temperature and UV. The on-set of mould growth on selected wood materials (pine, spruce, larch, oak, aspen, thermally treated pine) have been studied in 8 different controlled climate conditions, where two levels of surface condensation regimes have been mimicked to capture time-of-wetness. Wood samples in conditions with high RH (85%) and longest time-of-wetness had the earliest on-set of mould growth, and under these conditions temperature (25°C/10°C) had no significant impact. Generally aspen had the earliest on-set of mould growth and thermally treated pine the latest. Data on on-set of mould growth will be incorporated in the simulation model to enable a knowledge-based decision tool regarding design and material selection.