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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2025

Sammendrag

Denne rapporten gir en analyse av metanpotensialet i ulike organiske fraksjoner som husdyrgjødsel, fiskeslam og industrifraksjoner. Den beskriver biogassproduksjonens prosesser og utfordringer, inkludert tekniske beregninger og vurderinger av innholdet av tørrstoff (TS), organisk tørrstoff (VS), og kjemisk oksygenbehov (COD). Formålet med rapporten er å vurdere potensialet for energiutvinning og bærekraftig ressursbruk gjennom biogassproduksjon, samt å identifisere og løse utfordringer knyttet til bruk av ulike substrater i biogassreaktorer. Rapporten er utarbeidet i prosjektet Biogass og energipotensiale i husdyrgjødsel (pnr 701).

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Sammendrag

Humic acid (HA) is an inhibitor that can diminish bioconversion during anaerobic digestion (AD). In this study, a non-metallic nanomaterial, N-doped carbon quantum dots (NCQD), was synthesized to alleviate the HA inhibition, followed by exploring its mechanism. Adding 500 mg/L NCQD prevented HA inhibition by binding to HA or microorganism surfaces, thus avoiding contact between them. This phenomenon, known as shielding inhibition, involves the formation of hydrogen bonds and chemical bonds. The intermolecular force and dissociation constant (KD) between NCQD and HA were determined to be 112.83 ±3.98 nN and 1.0 ±0.07 ×10 7 M, respectively. NCQD could promote electron transfer in AD systems. According to the in-depth microbial analysis, NCQD significantly enhanced the metabolic pathways of methanogenesis and biosynthesis of coenzyme F420. This groundbreaking research demonstrates the dual functionality of NCQD by effectively shielding against HA inhibition and promoting electron transfer. The study also unravels the mechanism of interface interaction, electron transfer, and metabolic pathways, leading to significant breakthroughs in addressing HA inhibition.

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Sammendrag

In-situ biomethanation is an efficient process for converting carbon dioxide (CO2) to methane (CH4) using hydrogen (H2) alongside anaerobic digestion (AD) process. However, AD of protein rich substrate often leads to the accumulation of ammonia nitrogen at high concentration. As a major inhibitor, this accumulation affects not only the AD process but also in-situ biomethanation. This study investigated the impact of ammonia nitrogen (0.5–5 g/L) on biomethanation performance using anaerobic moving-bed biofilm reactors (AnMBBRs). Without biofilm/biocarrier support, methane production was significantly inhibited above 3 g/L of ammonia nitrogen. In contrast, AnMBBR maintained high methane yields of 156.5 NmL/Lreactor at 2.5 g/L and 151.3 NmL/Lreactor at 5 g/L ammonia nitrogen, representing increases of 49 % and 76 %, respectively, compared to reactors without biofilm. Microbial analysis via 16S rRNA sequencing showed that Methanothermobacter, a thermophilic hydrogenotrophic methanogen, increased in relative abundance under ammonia nitrogen stress, which was further supported by carbon isotope analysis. Overall, these results highlighted the potential of AnMBBR to overcome ammonia nitrogen stress in in-situ biomethanation.

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Sammendrag

Syngas biomethanation represents a promising pathway to convert recalcitrant feedstocks into biomethane. However, the hydrogen (H2) content in syngas is often insufficient or fluctuates, which affects the overall performance. This study evaluated the effect of H2 addition on syngas conversion efficiency and microbial community dynamics using two trickle bed reactors (TBRs). One TBR was fed with syngas, while another received syngas supplemented with H2. Both TBRs demonstrated the feasibility of converting CO from syngas to methane, with the H2 supplemented TBR outperforming the syngas-only TBR. The H2 supplemented TBR achieved over 90 % conversion rate at a gas loading rate of 15 NL/Lreactor/d and reached peak methane production at a gas loading rate at 20 NL/Lreactor/d. Microbial community structure analysis revealed a dominance of Methanobacterium, a known thermophilic hydrogenotrophic methanogen. Although H2 addition enhanced performance, a decline in conversion efficiency at higher gas loading rates highlights the need for further optimization.