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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2024

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Sammendrag

Denne rapporten oppsummerer resultatene fra analyser av elgens forflytnings- og trekkmønster og leveområder for 12 GPS merka elger i Stabbursdalen nasjonalpark i Porsanger kommune i 2018. Ut fra GPS-elgenes posisjoner ble det analysert forflytningsmønster, trekkdistanser, trekkruter og tidspunkt for vår- og høsttrekket, leveområdestørrelse i løpet av året og i jakttida og bruken av ulike habitatkategorier i løpet av året. I tillegg ble elgens forflytningsmønstre i forhold til inndelingen i bestandsplanområder og jaktfelt i regionen analysert. Totalt sett gir studien viktig innsikt i elgens arealbruk og kan bidra til bedre forvaltning og bevaring av elgbestanden og å sikre furuskogen i Stabbursdalen.

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This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free procedure using two raspberry varieties. Regrowth rate of the raspberry variety ‘Tulameen’ after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety ‘Autumn First’, the regrowth level was 73%. In vitro shoots of the cryopreserved variety ‘Tulameen’ were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of ‘Tulameen’ by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.

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Parasitic nematodes are ubiquitous and can negatively impact their host by reducing fecundity or increasing mortality, yet the driver of variation in the parasite community across a wildlife host's geographic distribution remains elusive for most species. Based on an extensive collection of fecal samples (n = 264) from GPS marked moose (Alces alces), we used DNA metabarcoding to characterize the individual (sex, age class) and seasonal parasitic nematode community in relation to habitat use and migration behavior in five populations distributed across a wide latitudinal gradient (59.6°N to 70.5°N) in Norway. We detected 21 distinct nematode taxa with the six most common being Ostertagia spp., Nematodirella spp., Trichostongylus spp., T. axei, Elaphostrongylus alces, and an unclassified Strongylida. There was higher prevalence of livestock parasites in areas with larger sheep populations indicating a higher risk of spillover events. The individual level nematode richness was mostly consistent across study areas, while the number and type of nematode taxa detected at each study area varied considerably but did not follow a latitudinal gradient. While migration distance affected nematode beta-diversity across all sites, it had a positive effect on richness at only two of the five study areas suggesting population specific effects. Unexpectedly, nematode richness was higher in winter than summer when very few nematodes were detected. Here we provide the first extensive description of the parasitic nematode community of moose across a wide latitudinal range. Overall, the population-specific impact of migration on parasitism across the distribution range and variation in sympatry with other ruminants suggest local characteristics affect host-parasite relationships.