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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2023

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Craft breweries are continuously searching for beers made with locally produced raw materials and unique flavor profiles to respond to consumer requests. We explored the behavior of three commercial strains of Saccharomyces cerevisiae in the fermentation of ale beer with a high prevalence of rye malt in comparison to pure barley malt. In total, 34 volatile organic compounds were identified, with esters and alcohols being the quantitatively most abundant classes. The yeast strain appeared to impart more differences in the beer’s volatile profile compared to malt. In particular, S. cerevisiae var. diastaticus Y2 strain was associated with a higher production of esters, while strain S. cerevisiae Y3 was correlated to the higher amounts of terpenes together with the lowest relative abundance of volatile acids. Our findings encourage further investigation of the fermentation performance of several yeast strains to produce beers with unique flavors.

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Utilizing forest ecosystems to mitigate climate change effects and to preserve biodiversity requires detailed insights into the feedbacks between forest type, climatic and soil conditions, and in particular forest management history and practice. Analysis of long-term observations at the site level, remote sensing proxies and understanding relevant biogeochemical and biophysical processes are key to achieving these insights. In the recently started EU H2020 project “CLimate Mitigation and Bioeconomy pathways for sustainable FORESTry” (CLIMB-FOREST), we address these issues based on intensely monitored sites with flux measurements (ICOS, Fluxnet), other ecosystem research and observation networks (eLTER, National Forest Inventories), remotely sensed observations and process understanding. This presentation outlines the activities of CLIMB-FOREST regarding (1) carbon stocks and fluxes according to stand age, species distribution, management and disturbance history; (2) biophysical effects of forest structure; (3) effects and importance of short-lived climate forcers (e.g. BVOCs) and (4) management and extreme event (drought, fire) impact on SOC and N dynamics. We also outline how the gained knowledge informs scenario runs of the Vegetation and Earth System Model RCA-GUESS in the project.

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Rapporten oppsummer resultater fra miljøovervåking av Spillhaug avfallsdeponi for driftsåret 2022. Data vurderes mot rensekrav og tidligere undersøkelser. Deponiet er etablert i et tidligere sandtak uten bunntetting. Vannet strømmer 2-300 m gjennom sand avgrenset av fjell før sigevannsforurenset grunnvann pumpes til et behandlingsanlegg. Renseparken omfatter brønner med pumpe, luftebasseng og tre tilplantede våtmarksbasseng. Grunnvannsmagasinet inngår som en del av rensesystemet for sigevann. Sigevannsmengde gjennom renseparken er i 2022 målt til 25 916 m3 som er på nivå med tidligere år i forhold til årsnedbøren (583 mm). Beregnet ut fra endring i vannkvalitet fra deponiet og til resipienten Sandbekken, har rensegraden vært: 99% for jern, 87% for KOF, 86% for nitrogen (tot-N) og 94% for ammonium nitrogen. Nivået av miljøgifter i utløpet av renseanlegget er lavt, og konsentrasjoner av tungmetaller er hovedsakelig under terskelverdier som anses å være skadelige. Sandbekken, som mottar renset vann og diffus innlekking via grunnvann og overvann påvirkes av sigevann med økte konsentrasjoner av konduktivitet og nitrogen, men kun liten endring for de andre analyserte parameterne. Årlig utslipp av PFAS forbindelser er ca 3 gram. Overvåkningen gir grunnlag for å fastslå at renseanlegget virker tilfredsstillende. Det er derfor ikke foreslått spesielle tiltak for å bedre rensingen i 2023. NIBIO foreslår at driftsoppfølging og overvåkning ligger på samme nivå som 2022, med kontroll av anleggets prosesser i ulike trinn for å dokumentere anleggets funksjon, utslipp og påvirkning av resipient.

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The Norwegian Scientific Committee for food an Environment (VKM) has assessed an application for authorisation of refined oilseed rape oil (Aquaterra®) derived from genetically modified oilseed rape line NS-B50027-4 for exclusive use as an ingredient in fish feed in Norway. NS-B50027-4 is also named DHA-canola. This report uses the term oilseed rape. NS-B50027-4 produces omega-3 long-chain (≥C20) polyunsaturated fatty acids (omega-3 LC-PUFAs) in its seeds, with a high level of docosahexaenoic acid (DHA) and a small amount of eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA). Aquaterra® also contains a significant level of alpha-Linolenic acid (ALA). Whereas ALA can be derived from plants, the primary producers of EPA and DHA are mainly marine microalgae. EPA and DHA are concentrated in the food chain to fish in the oceans and are often referred to as marine omega-3 fatty acids. NS-B50027-4 was developed as an alternative land-based source of marine fatty acids, mainly DHA. NS-B50027-4 was genetically modified to express seven transgenes derived from yeasts and marine microalgae that encode the enzymes necessary for the biosynthesis of omega-3 long chain polyunsaturated fatty acids. In addition, an eighth gene, pat, was inserted as a marker for selection purposes during development. The pat gene encodes the enzyme phosphinothricin N-acetyltransferase (PAT) conferring tolerance to glufosinate-ammonium herbicides. Equally to conventional refined oilseed rape oils any residues levels of proteins, including the introduced enzymes, will be negligible in the Aquaterra® oil. The risk assessment of Aquaterra® was conducted in accordance with the guidance for risk assessment of derived food and feed from genetically modified plants as described by the European Food Safety Authority (EFSA, 2011a). The risk assessment is based primarily on scientific documentation provided in the application EFSA-GMO-NL-2019-160, which seeks approval for NS-B50027-4 for all applicable food and feed uses in the European Union (EU). VKM concludes that the provided scientific documentation fulfills the criteria of the EFSA guidance and is adequate for risk assessment. VKM concludes that the molecular characterisation, comparative, nutritional, toxicological and allergenicity assessments of NS-B50027-4 do not indicate increased risks to animal or human health compared to its conventional counterpart (comparator) or commercial reference varieties. Based on this together with specific analyses of the seed oil fraction and studies, e.g., in fish, VKM therefore concludes that the refined oil Aquaterra®, is equal to conventional oils from oilseed rape except for the altered composition in fatty acids. VKM concludes there is no increased health risk to fish fed Aquaterra® in feed compared to conventional feeds with oils from other sources, nor is there an indication of increased risk to the environment. Since Aquaterra® is equal to conventional oils from oilseed rape except for the marine omega-3 fatty acids already present in fish feeds, VKM concludes there is no greater need for health or environmental monitoring of feeds containing Aquaterra® than conventional feeds.

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The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-Ocaffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.

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Drought-induced mortality is a major direct effect of climate change on tree health, but drought can also affect trees indirectly by altering their susceptibility to pathogens. Here, we report how a combination of mild or severe drought and pathogen infection affected the growth, pathogen resistance and gene expression in potted 5-year-old Norway spruce trees [Picea abies (L.) Karst.]. After 5 weeks of drought, trees were inoculated with the fungal pathogen Endoconidiophora polonica. Combined drought–pathogen stress over the next 8 weeks led to significant reductions in the growth of drought-treated trees relative to well-watered trees and more so in trees subjected to severe drought. Belowground, growth of the smallest fine roots was most affected. Aboveground, shoot diameter change was most sensitive to the combined stress, followed by shoot length growth and twig biomass. Both drought-related and some resistance-related genes were upregulated in bark samples collected after 5 weeks of drought (but before pathogen infection), and gene expression levels scaled with the intensity of drought stress. Trees subjected to severe drought were much more susceptible to pathogen infection than well-watered trees or trees subjected to mild drought. Overall, our results show that mild drought stress may increase the tree resistance to pathogen infection by upregulating resistance-related genes. Severe drought stress, on the other hand, decreased tree resistance. Because drought episodes are expected to become more frequent with climate change, combined effects of drought and pathogen stress should be studied in more detail to understand how these stressors interactively influence tree susceptibility to pests and pathogens.

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The commercial apple production in Norway is limited to the small regions along the fjords in the southwest part of the country and around lakes or near the sea in the southeast with favorable climate. Due to the rapid rate of climate change over the recent decades, it is expected that suitable heat conditions for apple growing will expand to the areas that were previously too cold. This study analyses the heat suitability of future climate (2021–2100) under the RCP8.5 scenario for 6 common apple varieties in Norway: Discovery, Gravenstein, Summerred, Aroma, Rubinstep and Elstar. Previously established heat requirement criteria (based on the temperature threshold for the full blooming and growing degree days sum between the full bloom and harvest) are applied to the temperature outputs of the regional climate models downscaled to 1 km resolution. The assessment indicates that as temperature rises, heat conditions suitable for cultivation of all 6 apple varieties will expand. According to the ensemble median value, areas with the favorable heat conditions for growing at least one of the considered apple varieties will increase 25 times in the period 2021–2040 and 60 times in the period 2041–2060, compared to the referent period 1971–2000. At the same time, areas suitable for all 6 apple varieties will increase 3 times in the first, and 3.8 times in the latter period. The favorable areas will advance from south and southeast northwards and inland in the eastern region, along the west and northwestern coastline towards higher latitudes, and along continental parts of fjords. The fastest expansion of heat suitable conditions is expected for Discovery and Gravenstein. The findings of this study are relevant for zoning apple production future potential and for strategical planning of climate change adaptation measures within the sector. Weather-related risks, such as risks from winter low temperatures, spring frost, drought and extreme precipitation were not considered.

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Comprehensive livestock tracking and behavioral characterization in extensive systems is technically challenging and expensive. Some technologies and data strategies based around proximity information may be more affordable. This paper brings together experiences from two major PLF projects involving cattle in extensive U.S. rangelands and sheep in extensive UK mountains and considers proximity technology for two resources, water in dry rangelands, and supplementary feed in pregnancy, respectively. Opportunities to characterize useful livestock variables include presence/absence, diurnal patterns, use of resources and changing use patterns. Results covering supplementary feed, used fixed Bluetooth Low Energy (BLE) readers arrayed around feeding points, 48 Blackface and 50 Lleyn ewes on 33ha of grazing that wore small (c14 g) BLE beacons. Beacons on ewes communicated identity and RSSI (Received Signal Strength Indicator) via receiving readers, pushing data in near-real time via LPWAN to an ArCGIS Online database. Differences in proximity at feeding areas were found for breed and age and patterns of activity over 24-hour periods, supporting the view that BLE technology covering only proportions of grazing areas could be useful for management purposes. For water access in arid rangelands, 11 cows in a 480ha paddock wore NoFence virtual fencing collars with GNSS real-time tracking using cellphone communications. Daily patterns of proximity to the only water source derived from GNSS data support the view that useful information could be provided by BLE proximity systems at lower cost than GNSS collars. Proximity approaches alone provides less information than GNSS systems.

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The COVID-19 pandemic, surprised many through its impact on the food systems, resulting in collapses in the food production value chains and in the integrated pest disease management sector with fatal outcomes in many places. However, the impact of COVID-19 and the digital experience perspective on Integrating Pest Management (IPM) is still yet to be understood. In Africa, the impact was devastating, mostly for the vulnerable smallholder farm households, who were rendered unable to access markets to purchase inputs and sell their produce during the lockdown period. By using a holistic approach the paper reviews different Information and Communications Technologies (ICTs), digitalization, and how this enhanced the capacity of smallholder farmers resilient, and inform their smart-IPM practices in order to improve food systems' amidst climate change during and in the post-COVID-19 period. Different digital modalities were adopted to ensure continuous food production, access to inputs and finances, and selling surplus production among others. This was largely possible by using ICTs to deliver these needed services digitally. The study shares contributions and capacity perspectives of ICTs for empowering smallholder farmers to boost the resilience of their food systems based on COVID-19 successful experiences. Thus digital solutions must be embraced in the delivery of extension service on pest management and good agronomic practices, money transfers for purchasing inputs, receiving payment for sold farm produce, and markets information exchange. These are key avenues through which digital solutions strategically supported smallholder-based food systems through the pandemic.

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The aim of this study was to evaluate whether sea lice grazing efficiency, behaviour, size variation and cataract development can be improved through selective breeding of lumpfish. A series of studies was conducted over a four-year period where distinctive lumpfish families were established initially from wild caught mature fish and latterly from established breeding lines. Four subsequent trials (called: Phase I-IV) with ten families of lumpfish (N = 480) with a mean (± SD) weight of 46.4 ± 9.4 g (Phase I), 54.8 ± 9.2 g (Phase II), 42.0 ± 7.4 g (Phase III) and 31.3 ± 2.4 g (Phase IV) were distributed among ten sea cages (5 × 5 × 5 m) during autumn 2018 to spring 2022, each stocked with 400–404 Atlantic salmon with an average initial mean (± SD) of 387 ± 9 g (Phase I), 621 ± 15 g (Phase II), 280 ± 16 g (Phase III) and 480 ± 66 g (Phase IV). All the ten cages were stocked with 48 lumpfish (12% stocking density). In all phases there was a large inter-family variation of lice grazing of lumpfish of both L. salmonis and C. elongatus. When sea lice grazing was scaled in relation to sea lice infestation numbers on the salmon the highest sea lice grazing activity was found in Phase IV and in particular in families sired from farmed parents. There was a general trend for mean start weights and standard deviations to decrease as the phases continued. A significant increase was found in frequency of behaviour associated with feeding on natural food sources and grazing sea lice from salmon during each subsequent phase. The increase in incidence of cataracts from start to end of each trial phase was significantly reduced from Phase I (16%) to Phase IV (2%). Overall, present findings showed that sea lice grazing of both L. salmonis and C. elongatus, size variation, cataract prevalence and behaviour types can be enhanced through selection and targeted breeding programs.