Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2020
Sammendrag
The emergence of antibiotic-resistant pathogens has caused a serious worldwide problem in infection treatment in recent years. One of the pathogens is methicillin-resistant Staphylococcus aureus (MRSA), which is a major cause of skin and soft tissue infections. Alternative strategies and novel sources of antimicrobials to solve antibiotic resistance problems are urgently needed. In this study, we explored the potential of two broad-spectrum bacteriocins, garvicin KS and micrococcin P1, in skin infection treatments. The two bacteriocins acted synergistically with each other and with penicillin G in killing MRSA in vitro. The MICs of the antimicrobials in the three-component mixture were 40 ng/ml for micrococcin P1 and 2 μg/ml for garvicin KS and penicillin G, which were 62, 16, and at least 1,250 times lower than their MICs when assessed individually. To assess its therapeutic potential further, we challenged the three-component formulation in a murine skin infection model with the multidrug-resistant luciferase-tagged MRSA Xen31, a strain derived from the clinical isolate S. aureus ATCC 33591. Using the tagged-luciferase activity as a reporter for the presence of Xen31 in wounds, we demonstrated that the three-component formulation was efficient in eradicating the pathogen from treated wounds. Furthermore, compared to Fucidin cream, which is an antibiotic commonly used in skin infection treatments, our formulation was also superior in terms of preventing resistance development.
Sammendrag
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Forfattere
Hye-Seon Kim Jessica M. Lohmar Mark Busman Daren W. Brown Todd A. Naumann Hege Divon Erik Lysøe Silvio Uhlig Robert H. ProctorSammendrag
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Forfattere
Hye-Seon Kim Jessica M. Lohmar Mark Busman Daren W. Brown Todd A. Naumann Hege Hvattum Divon Erik Lysøe Silvio Uhlig Robert H. ProctorSammendrag
Background Sphingolipids are structural components and signaling molecules in eukaryotic membranes, and many organisms produce compounds that inhibit sphingolipid metabolism. Some of the inhibitors are structurally similar to the sphingolipid biosynthetic intermediate sphinganine and are referred to as sphinganine-analog metabolites (SAMs). The mycotoxins fumonisins, which are frequent contaminants in maize, are one family of SAMs. Due to food and feed safety concerns, fumonisin biosynthesis has been investigated extensively, including characterization of the fumonisin biosynthetic gene cluster in the agriculturally important fungi Aspergillus and Fusarium. Production of several other SAMs has also been reported in fungi, but there is almost no information on their biosynthesis. There is also little information on how widely SAM production occurs in fungi or on the extent of structural variation of fungal SAMs. Results Using fumonisin biosynthesis as a model, we predicted that SAM biosynthetic gene clusters in fungi should include a polyketide synthase (PKS), an aminotransferase and a dehydrogenase gene. Surveys of genome sequences identified five putative clusters with this three-gene combination in 92 of 186 Fusarium species examined. Collectively, the putative SAM clusters were distributed widely but discontinuously among the species. We propose that the SAM5 cluster confers production of a previously reported Fusarium SAM, 2-amino-14,16-dimethyloctadecan-3-ol (AOD), based on the occurrence of AOD production only in species with the cluster and on deletion analysis of the SAM5 cluster PKS gene. We also identified SAM clusters in 24 species of other fungal genera, and propose that one of the clusters confers production of sphingofungin, a previously reported Aspergillus SAM. Conclusion Our results provide a genomics approach to identify novel SAM biosynthetic gene clusters in fungi, which should in turn contribute to identification of novel SAMs with applications in medicine and other fields. Information about novel SAMs could also provide insights into the role of SAMs in the ecology of fungi. Such insights have potential to contribute to strategies to reduce fumonisin contamination in crops and to control crop diseases caused by SAM-producing fungi.
Forfattere
Kerry O'Donnell Abdullah M. S. Al-Hatmi Takayuki Aoki Balázs Brankovics José F. Cano-Lira Jeffrey J. Coleman G. Sybren de Hoog Antonio Di Pietro Rasmus J. N. Frandsen David M. Geiser Connie F. C. Gibas Josep Guarro Hye-Seon Kim H. Corby Kistler Imane Laraba John F. Leslie Manuel S. López-Berges Erik Lysøe Jacques F. Meis Michel Monod Robert H. Proctor Martijn Rep Carmen Ruiz-Roldan Adnan Šišic Jason E. Stajich Emma T. Steenkamp Brett A. Summerell Theo A. J. van der Lee Anne D. van Diepeningen Paul E. Verweij Cees Waalwijk Todd J. Ward Brian L. Wickes Nathan P. Wiederhold Michael J. Wingfield Ning Zhang Sean X. ZhangSammendrag
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Forfattere
David M. Geiser Abdullah M. S. Al-Hatmi Takayuki Aoki Tsutomu Arie Virgilio Balmas Irene Barnes Gary C. Bergstrom Madan K. Bhattacharyya Cheryl L. Blomquist Robert L. Bowden Balázs Brankovics Daren W. Brown Lester W. Burgess Kathryn Bushley Mark Busman José F. Cano-Lira Joseph D. Carrillo Hao-Xun Chang Chi-Yu Chen Wanquan Chen Martin Chilvers Sofia Chulze Jeffrey J. Coleman Christina A. Cuomo Z. Wilhelm De Beer G. Sybren de Hoog Johanna Del Castillo-Múnera Emerson M. Del Ponte Javier Diéguez-Uribeondo Antonio Di Pietro Véronique Edel-Hermann Wade H. Elmer Lynn Epstein Akif Eskalen Maria Carmela Esposto Kathryne L. Everts Sylvia P. Fernández-Pavía Gilvan Ferreira da Silva Nora A. Foroud Gerda Fourie Rasmus J. N. Frandsen Stanley Freeman Michael Freitag Omer Frenkel Kevin K. Fuller Tatiana Gagkaeva Donald M. Gardiner Anthony E. Glenn Scott E. Gold Thomas R. Gordon Nancy F. Gregory Marieka Gryzenhout Josep Guarro Beth K. Gugino Santiago Gutierrez Kim E. Hammond-Kosack Linda J. Harris Mónika Homa Cheng-Fang Hong László Hornok Jenn-Wen Huang Macit Ilkit Adriaana Jacobs Karin Jacobs Cong Jiang María del Mar Jiménez-Gasco Seogchan Kang Matthew T. Kasson Kemal Kazan John C. Kennell Hye-Seon Kim H. Corby Kistler Gretchen A. Kuldau Tomasz Kulik Oliver Kurzai Imane Laraba Matthew H. Laurence Theresa Lee Yin-won Lee Yong-Hwan Lee John F. Leslie Edward C.Y. Liew Lily W. Lofton Antonio F. Logrieco Manuel S. López-Berges Alicia G. Luque Erik Lysøe Li-Jun Ma Robert E. Marra Frank N. Martin Sara R. May Susan P. McCormick Chyanna McGee Jacques F. Meis Quirico Migheli N. M. I. Mohamed Nor Michel Monod Antonio Moretti Diane Mostert Giuseppina Mulè Françoise Munaut Gary P. Munkvold Paul Nicholson Marcio Nucci Kerry O’Donnell Matias Pasquali Ludwig H. Pfenning Anna Prigitano Robert H. Proctor Stéphane Ranque Stephen A. Rehner Martijn Rep Gerardo Rodríguez-Alvarado Lindy Joy Rose Mitchell G. Roth Carmen Ruiz-Roldan Amgad A. Saleh Baharuddin Salleh Hyunkyu Sang María Mercedes Scandiani Jonathan Scauflaire David G. Schmale III Dylan P. G. Short Adnan Šišić Jason A. Smith Christopher W. Smyth Hokyoung Son Ellie Spahr Jason E. Stajich Emma T. Steenkamp Christian Steinberg Rajagopal Subramaniam Haruhisa Suga Brett A. Summerell Antonella Susca Cassandra L. Swett Christopher Toomajian Terry J. Torres-Cruz Anna M. Tortorano Martin Urban Lisa J. Vaillancourt Gary E. Vallad Theo A. J. van der Lee Dan Vanderpool Anne D. van Diepeningen Martha M. Vaughan Eduard Venter Marcele Vermeulen Paul E. Verweij Altus Viljoen Cees Waalwijk Emma C. Wallace Grit Walther Jie Wang Todd J. Ward Brian L. Wickes Nathan P. Wiederhold Michael J. Wingfield Ana K. M. Wood Jin-Rong Xu Xiao-Bing Yang Tapani Yli-Mattila Sung-Hwan Yun Latiffah Zakaria Hao Zhang Ning Zhang Sean X. Zhang Xue ZhangSammendrag
Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. Previously (Geiser et al. 2013; Phytopathology 103:400-408. 2013), the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani Species Complex (FSSC). Subsequently, this concept was challenged by one research group (Lombard et al. 2015 Studies in Mycology 80: 189-245) who proposed dividing Fusarium into seven genera, including the FSSC as the genus Neocosmospora, with subsequent justification based on claims that the Geiser et al. (2013) concept of Fusarium is polyphyletic (Sandoval-Denis et al. 2018; Persoonia 41:109-129). Here we test this claim, and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species recently described as Neocosmospora were recombined in Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural and practical taxonomic option available.
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Forfattere
Paal KrokeneSammendrag
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