Tatsiana Espevig
Forsker
(+47) 406 23 778
tatsiana.espevig@nibio.no
Sted
Landvik
Besøksadresse
Reddalsveien 215, 4886 Grimstad
Vedlegg
CVLenker
Disease diagnosticsBiografi
Forskningsområder:
- Gress til grøntanlegg
- Integrert plantevern med fokus på myntflekk og mikrodochium flekk
- Vinterstyrke
- Abiotiske stressfaktorer (is, vann, temperatur)
- Etablering, reetablering og skjøtsel av gress til grøntanlegg
Tjenester:
- Diagnose av sykdommer på gress
Utdanning:
PhD in Agricultural Sciences at National Academy of Sciences of Belarus (2004) and in Plant Pathology at Norwegian University of Life Sciences, UMB (2011).
Forfattere
Tatsiana Espevig Kristine Sundsdal Victoria Stornes Moen Kate Entwistle Marina Usoltseva Sabine Braitmaier Daniel Hunt Carlos Guerrero Monica Skogen Erik LysøeSammendrag
Thirty-seven turfgrass samples expressing dollar spot symptoms were collected in summer 2020 on golf courses in Sweden, Denmark, United Kingdom, Germany, Portugal, and Spain. The fungi were isolated at Norwegian Institute of Bioeconomy Research (NIBIO) Turfgrass Laboratory (Norway) and sent for molecular identification using sequencing of regions of ITS (internal transcribed regions of the ribosomal DNA) and calmodulin. Clarireedia homoeocarpa was identified in four turfgrass samples and Clarireedia jacksonii was identified in 11 turfgrass samples. From seven turfgrass samples, the isolated fungi were not Clarireedia spp., but Waitea circinata, Fusarium culmorum, and Fusarium oxysporum. This suggests dollar spot is not always accurately identified from foliar symptoms in the field.
Forfattere
Anna Antropova Yuri Lebedin Valentina Maygurova Marina Usoltseva Tatiana Gagkaeva Tatsiana EspevigSammendrag
Det er ikke registrert sammendrag
Forfattere
Yuri Lebedin Anna Antropova Valentina Maygurova Marina Usoltseva Tatiana Gagkaeva Tatsiana EspevigSammendrag
The most common and harmful disease affecting the grass on golf courses in the Nordic countries is microdochium patch. The early diagnosis of the Microdochium nivale can help prevent the spread of infection through targeted treatment. The aim of the work was to develop an enzyme linked immunosorbent assay (ELISA) test system for Microdochium fungi detection. We have prepared specific rabbit affinity antibodies against Microdochium genus by antigen adsorption and exhaustion on wide range of fungal species. These specific antibodies were used to construct sandwich ELISA showing genus specificity and capable to detect the antigen on early stage of infection on different grass substrates. In field study, the ELISA has shown good correlation to microbiological diagnostics and was able to detect the latent infection in the absence of visual signs. We suggest that Microdochium ELISA can be used for regular testing of grass specimens for prediction and early diagnosis of latent infection. Further studies are required to determine the antigen level, which indicates the degree of infection at which steps to prevent the disease need to be applied.