Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Andre van Eerde Aniko Varnai John-Kristian Jameson Lisa Paruch Anders Moen Jan Haug Anonsen Piotr Chylenski Hege Særvold Steen Inger Heldal Ralph Bock Vincent Eijsink Jihong Liu ClarkeSammendrag
Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
Forfattere
Jihong Liu ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Marit Almvik Marianne Stenrød Randi Bolli Alice Budai Ingvill Hauso Olaug Bach Steinar Haugse Ole Martin EkloSammendrag
Det er ikke registrert sammendrag
Forfattere
Marit Almvik Marianne Stenrød Randi Bolli Alice Budai Ingvill Hauso Olaug Bach Steinar Haugse Ole Martin EkloSammendrag
OBJECTIVES • Gain a better understanding of the fate of pesticides in the environment by also screening and detecting their metabolites • Predict and detect pesticide metabolites in soils using high resolution accurate mass (HRAM) tools; Thermo Q Exactive orbitrap and Compound DiscovererTM software. HIGHLIGHTS • We present in silico metabolism simulation to predict fungicide metabolites in soil • We present a screening method for 800 pesticides and metabolites in soil and food, exemplified with soil samples from strawberry field degradation studies (including fluopyram, boscalid and pyraclostrobin and others) • We address the lack of molecular formulas for known metabolites in current databases as an obstacle in establishing HRAM screening methods
Forfattere
Volkmar Timmermann Kjell Andreassen May Bente Brurberg Isabella Børja Nicholas Clarke Daniel Flø Jane Uhd Jepsen Torstein Kvamme Jørn-Frode Nordbakken Per Holm Nygaard Martin Pettersson Sverre Solberg Halvor Solheim Venche Talgø Ole Petter Laksforsmo Vindstad Gro Wollebæk Bjørn Økland Wenche AasSammendrag
Source at <a href=http://hdl.handle.net/11250/2616613>http://hdl.handle.net/11250/2616613</a>
Forfattere
Alice BudaiSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Daniel RasseSammendrag
Det er ikke registrert sammendrag