Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2018
Forfattere
Mihaela-Olivia Dobrica Catalin Lazar Lisa Paruch Andre van Eerde Jihong Liu Clarke Catalin Tucureanu Iuliana Caras Sonya Ciulean Adrian Onu Vlad Tofan Alexandru Branzan Stephan Urban Crina Stavaru Norica Branza-NichitaSammendrag
Hepatitis B Virus (HBV) infection can be prevented by vaccination. Vaccines containing the small (S)envelope protein are currently used in universal vaccination programs and achieve protective immuneresponse in more than 90% of recipients. However, new vaccination strategies are necessary for successfulimmunization of the remaining non- or low-responders. We have previously characterized a novel HBVchimeric antigen, which combines neutralization epitopes of the S and the preS1 domain of the large (L)envelope protein (genotype D). The S/preS121–47chimera produced in mammalian cells and Nicotianabenthamiana plants, induced a significantly stronger immune response in parenterally vaccinated micethan the S protein. Here we describe the transient expression of the S/preS121–47antigen in an edibleplant, Lactuca sativa, for potential development of an oral HBV vaccine. Our study shows that oral admin-istration of adjuvant-free Lactuca sativa expressing the S/preS121–47antigen, three times, at 1lg/dose,was sufficient to trigger a humoral immune response in mice. Importantly, the elicited antibodies wereable to neutralize HBV infection in an NTCP-expressing infection system (HepG2-NTCP cell line) moreefficiently than those induced by mice fed on Lactuca sativa expressing the S protein. These results sup-port the S/preS121–47antigen as a promising candidate for future development as an edible HBV vaccine.
Forfattere
Francesca Giampieri Sadia Afrin Derek Stewart Gordon J. McDougall Rex Brennan Lesley Blyth Massimiliano Gasparrini Luca Mazzoni Franco Capocasa José Miguel Alvarez-Suarez Stefano Bompadre Pedro Nogueira Brás de Oliveira Claudia N. Santos Manuel Masias Pablo Agudo Jorge Crespo Bruno Mezzetti Tamara Y. Forbes-Hernández Maurizio BattinoSammendrag
Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p < 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
Forfattere
Patryk Czortek Jutta Kapfer Anna Delimat Amy Eycott John-Arvid Grytnes Anna Orczewska Halina Ratyńska Antoni Zięba Bogdan JaroszewiczSammendrag
Mountain vegetation is often considered highly sensitive to climate and land-use changes due to steep environmental gradients determining local plant species composition. In this study we present plant species compositional shifts in the Tatra Mts over the past 90 years and discuss the potential drivers of the changes observed. Using historical vegetation studies of the region from 1927, we resurveyed 76 vegetation plots, recording the vascular flora of each plot using the same methodology as in the original survey. We used an indirect method to quantify plant species compositional shifts and to indicate which environmental gradients could be responsible for these shifts: by calculating shifts in estimated species optima as reflected in shifts in the ecological indicator values of co-occurring species. To find shifts in species composition, focusing on each vegetation type separately, we used ordination (DCA). The species optimum changed significantly for at least one of the tested environmental gradients for 26 of the 95 plant species tested; most of these species changed in terms of the moisture indicator value. We found that the strongest shifts in species composition were in mylonite grassland, snowbed and hygrophilous tall herb communities. Changes in precipitation and increase in temperature were found to most likely drive compositional shifts in vegetation resurveyed. It is likely that the combined effect of climate change and cessation of sheep grazing has driven a species composition shift in granite grasslands communities.
Sammendrag
Forestry in coastal Norway has traditionally been a marginal activity with a low annual harvest rate. However, the region is now faced with large areas of spruce plantations that will reach harvest maturity within the next 25 years. Due to the poor infrastructure in the region, the current challenge is to harvest the maturing spruce plantations at an acceptable cost. Hence, there is considerable interest both from the forest sector and politicians to invest in infrastructure that can provide the basis for profitable forest sector development in coastal Norway. This paper presents a mathematical optimization model for timber transportation from stump to industry. The main decision variables are location of quays, upgrade of public road links, the length of new forest roads, and when the investments should happen. The main objective is to provide decision support for prioritization of infrastructure investments. The optimization model is combined with a dynamical forest resource model, providing details on available volumes and costs. A case study for coastal Norway is presented and solved to optimality. The instance includes 10 counties comprising more than 200 municipalities with forest resources, 53 possible new quays for timber export and 916 public road links that also can be upgraded. Compared with a no investment case, the optimal solution improved the objective by 23%. The study shows that consistent, informative and good analyses can be performed to evaluate trade-offs, prioritization, time and order of investment, and cost saving potentials of infrastructure investments in the forest industry. The solution seems reasonable based on present infrastructure and state of the forest.
Forfattere
Ivan N. Bolotov Alexander A. Makhrov Mikhail Yu. Gofarov Olga V. Aksenova Paul Eric Aspholm Yulia V. Bespalaya Mikhail B. Kabakov Yulia S. Kolosova Alexander V. Kondakov Thomas Ofenböck Andrew N. Ostrovsky Igor Yu. Popov Ted von Proschwitz Mudite Rudzite Maris Rudzitis Svetlana E. Sokolova Ilmari Valovirta Ilya V. Vikhrev Maxim V. Vinarski Alexey A. ZotinSammendrag
The effects of climate change on oligotrophic rivers and their communities are almost unknown, albeit these ecosystems are the primary habitat of the critically endangered freshwater pearl mussel and its host fishes, salmonids. The distribution and abundance of pearl mussels have drastically decreased throughout Europe over the last century, particularly within the southern part of the range, but causes of this wide-scale extinction process are unclear. Here we estimate the effects of climate change on pearl mussels based on historical and recent samples from 50 rivers and 6 countries across Europe. We found that the shell convexity may be considered an indicator of the thermal effects on pearl mussel populations under warming climate because it reflects shifts in summer temperatures and is significantly different in viable and declining populations. Spatial and temporal modeling of the relationship between shell convexity and population status show that global climate change could have accelerated the population decline of pearl mussels over the last 100 years through rapidly decreasing suitable distribution areas. Simulation predicts future warming-induced range reduction, particularly in southern regions. These results highlight the importance of large-scale studies of keystone species, which can underscore the hidden effects of climate warming on freshwater ecosystems.
Forfattere
Björn Ringselle Therese With Berge Daniel Stout Tor Arvid Breland Paul E. Hatcher Espen Haugland Matthias Koesling Kjell Mangerud Tor Lunnan Lars Olav BrandsæterSammendrag
Docks (Rumex spp.) are a considerable problem in grassland production worldwide. We investigated how different cultural management techniques affected dock populations during grassland renewal: (I) renewal time, (II) companion crop, (III) false seedbed, (IV) taproot cutting (V), plough skimmer and (VI) ploughing depth. Three factorial split-split plot experiments were carried out in Norway in 2007–2008 (three locations), 2008–2009 (one location) and 2009 (one location). After grassland renewal, more dock plants emerged from seeds than from roots. Summer renewal resulted in more dock seed and root plants than spring renewal. Adding a spring barley companion crop to the grassland crop often reduced dock density and biomass. A false seedbed resulted in 71% fewer dock seed plants following summer renewal, but tended to increase the number of dock plants after spring renewal. In some instances, taproot cutting resulted in less dock biomass, but the effect was weak and inconsistent, and if ploughing was shallow (16 cm) or omitted, it instead increased dock root plant emergence. Fewer root plants emerged after deep ploughing (24 cm) compared to shallow ploughing, and a plough skimmer tended to reduce the number further. We conclude that a competitive companion crop can assist in controlling both dock seed and root plants, but it is more important that the renewal time is favourable to the main crop. Taproot cutting in conjunction with ploughing is not an effective way to reduce dock root plants, but ploughing is more effective if it is deep and a skimmer is used.
Sammendrag
Beiteskader fra gås, her grågås (Anser anser), er et økende problem for små, mellomstore og store årdsbruk flere steder i Norge. Beiteskadene har økt i takt med den økende bestanden av grågås de siste tiår, som kan skyldes en rekke faktorer, blant annet klimaendringer. Beiteskader fra gås kan deles inn i 1) tap av grovfôr (gress), 2) tap av spirer og såkorn, 3) store mengder avføring som reduserer kvaliteten på innhøstet fôr, 4) nedtråkking av gress/spirer og 5) spredning av frø fra uønskete planter og vekster. I dette prosjektet har vi kartlagt omfanget av beiteskader på grovfôrproduksjon på Musvær og estimert det økonomiske tapet gårdsdriften påføres. Musvær er en øygruppe som ligger ytterst mot havet, vest for Kvaløya i Troms. Gårdsdriften på Musvær har i de siste årene blitt mer påvirket av gåsebeiting. Som et avbøtende tiltak mot beiteskader har prosjektet sett på effekten av skadefelling av grågås, gjennomført etter en detaljert plan. Vi ønsket å estimere antallet grågås som minimum må felles for å oppnå en avbøtende effekt. Prosjektet konkluderer med at grågåsa på Musvær kan spise opp mot halvparten av gresset på et jorde og legge igjen betydelig mengder med ekskrementer om gjessene tillates å beite fritt. Et testforsøk viste at ekskrementer fra grågås kan ligge uberørt på et jorde i opptil 2 måneder og vil derfor kunne komme med i både første og andre slått. Tapet av grovfôr på det hardest beitede jordet utgjorde ca. NOK 30 000 estimert fra første og andre slått tilsammen, beregnet når det må erstattes med innkjøp av fullgodt erstatningsfôr. Siden dette jordet var så tilgriset av ekskrementer fra grågås at bøndene ikke vil høste det som fôr til husdyra sine, og jordet ansees som tapt, er det reelle tapet betydelig høyere. Uten skadefelling på nærliggende arealer vil antall gjess som beitet på dette arealet antakelig vært større og tapene enda større.....
Forfattere
Svein Solberg Johannes May Wiley Steven Bogren Johannes Breidenbach Torfinn Torp Belachew Gizachew ZelekeSammendrag
Monitoring changes in forest height, biomass and carbon stock is important for understanding the drivers of forest change, clarifying the geography and magnitude of the fluxes of the global carbon budget and for providing input data to REDD+. The objective of this study was to investigate the feasibility of covering these monitoring needs using InSAR DEM changes over time and associated estimates of forest biomass change and corresponding net CO2 emissions. A wall-to-wall map of net forest change for Uganda with its tropical forests was derived from two Digital Elevation Model (DEM) datasets, namely the SRTM acquired in 2000 and TanDEM-X acquired around 2012 based on Interferometric SAR (InSAR) and based on the height of the phase center. Errors in the form of bias, as well as parallel lines and belts having a certain height shift in the SRTM DEM were removed, and the penetration difference between X- and C-band SAR into the forest canopy was corrected. On average, we estimated X-band InSAR height to decrease by 7 cm during the period 2000–2012, corresponding to an estimated annual CO2 emission of 5 Mt for the entirety of Uganda. The uncertainty of this estimate given as a 95% confidence interval was 2.9–7.1 Mt. The presented method has a number of issues that require further research, including the particular SRTM biases and artifact errors; the penetration difference between the X- and C-band; the final height adjustment; and the validity of a linear conversion from InSAR height change to AGB change. However, the results corresponded well to other datasets on forest change and AGB stocks, concerning both their geographical variation and their aggregated values.
Forfattere
Olav Aaseth Hegnar Dejan Petrovic Bastien Bissaro Gry Alfredsen Aniko Varnai Vincent EijsinkSammendrag
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that perform oxidative cleavage of recalcitrant polysaccharides. We have purified and characterized a recombinant family AA9 LPMO, LPMO9B, from Gloeophyllum trabeum (GtLPMO9B) which is active on both cellulose and xyloglucan. Activity of the enzyme was tested in the presence of three different reductants: ascorbic acid, gallic acid, and 2,3-dihydroxybenzoic acid (2,3-DHBA). Under standard aerobic conditions typically used in LPMO experiments, the first two reductants could drive LPMO catalysis whereas 2,3-DHBA could not. In agreement with the recent discovery that H2O2 can drive LPMO catalysis, we show that gradual addition of H2O2 allowed LPMO activity at very low, substoichiometric (relative to products formed) reductant concentrations. Most importantly, we found that while 2,3-DHBA is not capable of driving the LPMO reaction under standard aerobic conditions, it can do so in the presence of externally added H2O2. At alkaline pH, 2,3-DHBA is able to drive the LPMO reaction without externally added H2O2, and this ability overlaps entirely the endogenous generation of H2O2 by GtLPMO9B-catalyzed oxidation of 2,3-DHBA. These findings support the notion that H2O2 is a cosubstrate of LPMOs and provide insight into how LPMO reactions depend on, and may be controlled by, the choice of pH and reductant.
Sammendrag
WOOL QUALITY OF NORWEGIAN WHITE SPÆL SHEEP BREED Lise GRØVA 1), Inger Anne Boman 2) 1) NIBIO, Norwegian Institute of Bioeconomy Research, Gunnars veg 6, 6630 Tingvoll, Norway; lise.grova@nibio.no 2) NSG, Norwegian sheep and goat association, Postboks 104, N-1431 Ås The Norwegian White Speal Sheep are characterised by their fluke-shaped and tapered short tail, dual-coated wool and the ability to thrive under harsh environmental conditions. The income in Norwegian sheep farming comes from lamb meat, wool and pelts. Today, wool is of minor economic importance, commonly accounting for less than 10% of the income. However, the interest of wool as a sustainable and local fibre is increasing. Wool quality traits of the Norwegian White spæl breed has been reported to be declining; i.e. an increase in medulated fibres and in kemp. To address this challenge, there has been developed and incorporated 1) wool quality assessment tutorials for farmers and breeders, 2) included heritability estimates of wool fleece weight and quality class in index calculations, and 3) conducted OFDA analysis of wool from breeding rams for three consecutive years. The work was initiated by the research-project KRUS - Enhancing local wool value chains in Norway (NFR 244618/E50) and has been carried out by NIBIO, the Norwegian Sheep Breeders Association (NSG), Animalia and Norilia. Wool quality assessment tutorials for farmers and breeders are available as a.pdf and video for free download from NSG webpage (nsg.no). A ‘wool evaluation kit’ with a magnifier is available for purchase, also from NSG. Implementing index estimates was made possible when including fleece weigh and fleece classification from all sheared lambs at slaughter into the Norwegain National Sheep Recording system. Further, OFDA analysis were conducted on wool from breeding rams collected autumn 2015, 2016 and 2017. The wool samples were analysed using the optical FD analyser (OFDA100; BSC Electronics Pty Ltd, Western Australia, Australia). OFDA analysis is conducted to describe wool quality traits, development of quality traits over time and also development of quality traits at different age of breeding rams. Preliminary results from this study will be presented at the conference. Keywords: sheep, wool quality, Norwegian white spæl, dual felt, double-coat