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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2020

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Sammendrag

Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. Previously (Geiser et al. 2013; Phytopathology 103:400-408. 2013), the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani Species Complex (FSSC). Subsequently, this concept was challenged by one research group (Lombard et al. 2015 Studies in Mycology 80: 189-245) who proposed dividing Fusarium into seven genera, including the FSSC as the genus Neocosmospora, with subsequent justification based on claims that the Geiser et al. (2013) concept of Fusarium is polyphyletic (Sandoval-Denis et al. 2018; Persoonia 41:109-129). Here we test this claim, and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species recently described as Neocosmospora were recombined in Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural and practical taxonomic option available.

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The stramenopile alga Nannochloropsis evolved by secondary endosymbiosis of a red alga by a heterotrophic host cell and emerged as a promising organism for biotechnological applications, such as the production of polyunsaturated fatty acids and biodiesel. Peroxisomes play major roles in fatty acid metabolism but experimental analyses of peroxisome biogenesis and metabolism in Nannochloropsis are not reported yet. In fungi, animals, and land plants, soluble proteins of peroxisomes are targeted to the matrix by one of two peroxisome targeting signals (type 1, PTS1, or type 2, PTS2), which are generally conserved across kingdoms and allow the prediction of peroxisomal matrix proteins from nuclear genome sequences. Because diatoms lost the PTS2 pathway secondarily, we investigated its presence in the stramenopile sister group of diatoms, the Eustigmatophyceae, represented by Nannochloropsis. We detected a full-length gene of a putative PEX7 ortholog coding for the cytosolic receptor of PTS2 proteins and demonstrated its expression in Nannochloropsis gaditana. The search for predicted PTS2 cargo proteins in N. gaditana yielded several candidates. In vivo subcellular targeting analyses of representative fusion proteins in different plant expression systems demonstrated that two predicted PTS2 domains were indeed functional and sufficient to direct a reporter protein to peroxisomes. Peroxisome targeting of the predicted PTS2 cargo proteins was further confirmed in Nannochloropsis oceanica by confocal and transmission electron microscopy. Taken together, the results demonstrate for the first time that one group of stramenopile algae maintained the import pathway for PTS2 cargo proteins. To comprehensively map and model the metabolic capabilities of Nannochloropsis peroxisomes, in silico predictions needs to encompass both the PTS1 and the PTS2 matrix proteome.

Sammendrag

The aim of this work was to calculate farm specific LCAs for milk-production on 200 dairy farms in Central Norway, where 185 farmed conventional and 15 according to organic standards. We assume that there are variations in environmental emission drivers between farms and therefore also variation in indicators. We think that information can be utilized to find management improvements on individual farms. Farm specific data on inputs and production for the calendar years 2014 to 2016 were used. The LCAs were calculated for purchased products and on farm-emissions, including atmospheric deposition, biological nitrogen fixation, use of fertilizer and manure. The enteric methane emission from digestion was calculated for different animal groups. The functional unit was one kg energy- corrected milk (ECM) delivered at farm-gate. For the 200 dairy farms there were huge variations of farm characteristics, environmental per- formance and economic outcome. On average, the organic farms produced milk with a lower carbon footprint (1.2 kg CO2 eq./kg ECM) than the conventional ones (1.4 kg CO2 eq./kg ECM). The organic farms had also a lower energy intensity (3.1 MJ/kg ECM) and nitrogen intensity (5.0 kg N/kg N) than their conventional colleagues (4.1 MJ/kg ECM and 6.9 kg N/kg N respectively). The contribution margin was better on the organic farms with 6.6 NOK/kg ECM compared to the conventional with 5.9 NOK/kg ECM. The average levels of the environmental indicators were comparable but slightly higher than findings in other international studies. The current study proved that the FARMnor model allows to calculate LCAs for large number of individual farms. The results show that the environmental performance and economic outcome vary between farms. We recommend that farm specific LCA-results are used to unveil what needs to be changed for improving a farm’s environmental performance.

Sammendrag

Mens andre rovdyr må ut på jakt hver gang de er sultne eller skal mate ungene, kan jerven hente kjøttbiter fra et av sine matlager. Ny studie viser at jerven gjerne bruker kjølige steder i naturen til å lagre kjøtt fra byttedyr eller åtsler.

Sammendrag

This study aims to identify some of the critically important factors in the sustainability of microbreweries in peripheral northern areas, focusing on the entrepreneurs’ understanding of sustainability. Theoretically, this study adopts the perspective of service-dominant logic on value. Methodologically, it uses an action-research approach and conducts in-depth interviews with four entrepreneurs. The findings suggest that the entrepreneurs reflect on several relevant issues in line with sustainability thinking. The perception of sustainability, especially environmental sustainability, is one subject that the entrepreneurs perceive and sometimes in conflict with the economic sustainability of their businesses. Constraints recognized include the lack of strategic planning and explicit discussions about sustainability with potential stakeholders. A critically important factor for the sustainability of microbreweries is the need for entrepreneurs to engage in wider discussions about the conceptual and practical aspects of sustainability, especially with government and community bodies.