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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2021

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The aim of this study was to demonstrate how aquacultural sludge can be processed and utilized as an organic nutrient solution (ONS) for hydroponic lettuce production. By using a previous developed method, approximately 80% of the processed sludge was reclaimed as a clear, nutrient-rich solution. The performance of the recovered nutrient solution on lettuce growth was assessed in a nutrient film hydroponic system. The results were compared to the results obtained using a conventional nutrient solution (CNS). Yield, fresh weight, water consumption, and nutrient and heavy metal content in leaf tissue were measured. In spite of a 16% lower average fresh weight obtained in ONS compared to the weight obtained in CNS, there was no statistical difference of the yield of lettuce among the two nutrient solutions. After the cultivation period, 90% of the lettuce heads grown in ONS exceeded the marked weight of 150 g. Foliar analysis revealed a similar or higher content of all nutrients, except of magnesium and molybdenum in the leaves of lettuce grown in the ONS compared to lettuce grown in the CNS. This study shows that nutrients recovered from aquacultural sludge can be utilized as fertilizer, thereby reducing the dependency on mineral fertilizer in hydroponic and aquaponic systems.

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Subclinical parasite infections in livestock production have huge economic impact on the farming industry, leading to decreased animal welfare and reduction in meat, milk and wool production. The global spread of anthelmintic resistance has exacerbated the threat of gastrointestinal nematode (GIN) infections to small ruminant health and production. Phytochemicals, such as condensed tannins (CT), have shown promising anthelmintic potential, and could complement other management practices to reduce anthelmintic use for parasite control. Conifer bark is a rich source of CT. Studies have shown that the content of CTs in Norway spruce and Scots pine varies, but on average they contain around 4% CTs. This study explored the anthelmintic activity of bark from Norwegian conifers over two seasons, extracted with three different solvents (water, acetone, methanol), against two sheep GIN, Teladorsagia circumcincta and Trichostrongylus colbriformis. Bark extracts were analysed to determine their total CT content and tested in vitro, with the aim to quantify their impact on egg hatching and L3 larval motility. Results show significant variation in efficacy of the extracts on egg hatching in a dose dependent manner. The CT content of the bark extracts were not always associated with LD50 values, indicating that other plant compounds may have additional anthelmintic effects. Preliminary results of the L3 larval motility test showed that incubation in the bark extracts had a smaller impact on this parasite stage at the concentrations tested. This research brings new insights into the potential anthelmintic activity of different phytochemicals and discusses the potential of bark extracts as a tool in the sustainable control of GIN in small ruminants.

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Water consumption along value chains of goods and services has increased globally and led to increased attention on water footprinting. Most global water consumption is accounted for by evaporation (E), which is connected via bridges of atmospheric moisture transport to other regions on Earth. However, the resultant source–receptor relationships between different drainage basins have not yet been considered in water footprinting. Based on a previously developed data set on the fate of land evaporation, we aim to close this gap by using comprehensive information on evaporation recycling in water footprinting for the first time. By considering both basin internal evaporation recycling (BIER; >5% in 2% of the world’s basins) and basin external evaporation recycling (BEER; >50% in 37% of the world’s basins), we were able to use three types of water inventories (basin internal, basin external, and transboundary inventories), which imply different evaluation perspectives in water footprinting. Drawing on recently developed impact assessment methods, we produced characterization models for assessing the impacts of blue and green water evaporation on blue water availability for all evaluation perspectives. The results show that the negative effects of evaporation in the originating basins are counteracted (and partly overcompensated) by the positive effects of reprecipitation in receiving basins. By aggregating them, combined net impacts can be determined. While we argue that these offset results should not be used as a standalone evaluation, the water footprint community should consider atmospheric moisture recycling in future standards and guidelines.

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The plant pathogenic fungus Fusarium graminearum is known to produce a wide array of secondary metabolites during plant infection. This includes several nonribosomal peptides. Recently, the fusaoctaxin (NRPS5/9) and gramilin (NRPS8) gene clusters were shown to be induced by host interactions. To widen our understanding of this important pathogen, we investigated the involvement of the NRPS4 gene cluster during infection and oxidative and osmotic stress. Overexpression of NRPS4 led to the discovery of a new cyclic hexapeptide, fusahexin (1), with the amino acid sequence cyclo-(d-Ala-l-Leu-d-allo-Thr-l-Pro-d-Leu-l-Leu). The structural analyses revealed an unusual ether bond between a proline Cδ to Cβ of the preceding threonine resulting in an oxazine ring system. The comparative genomic analyses showed that the small gene cluster only encodes an ABC transporter in addition to the five-module nonribosomal peptide synthetase (NRPS). Based on the structure of fusahexin and the domain architecture of NRPS4, we propose a biosynthetic model in which the terminal module is used to incorporate two leucine units. So far, iterative use of NRPS modules has primarily been described for siderophore synthetases, which makes NRPS4 a rare example of a fungal nonsiderophore NRPS with distinct iterative module usage.

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The objective of this study was to determine morphological and anatomical leaf characteristics and stomatal traits of three European (Pyrus communis L.) cultivars, three Asian [Pyrus pyrifolia (Burm. f.) Nakai] pear cultivars together with one interspecies hybrid (P. pyrifolia × P. communis ‘Bartlett’) and link them with the resistance to pathogens. Pear trees were grown under the standard practice without irrigation. Fully developed leaves were picked from the middle part of the extension shoots at the beginning of the July. Leaf traits were measured on leaves picked the same day. Anatomy of leaves was determined under light microscopy (LM) while scanning electron microscopy (SEM) was used for the examination of the stomata cells. Asian pear cultivars (‘Kousui’, ‘Nijisseiki’ and ‘Niitaka’) had much higher leaf parameters (width, length, stem length and leaf area) than the European cultivars (‘Conference’, ‘Williams Bartlett’, ‘Abbate Fetel’) and interspecies hybrid (‘Kieffer Seedling’). Midrib parameters (length and width) were the highest in ‘Kosui’ and ‘Nijisseiki’. Leaves of ‘Kieffer Seedling’ and ‘Abbate Fetel’ were the thickest, mainly due to increased palisade and spongy parenchyma thickness. The leaf stomata density significantly varied among the pear cultivars, ranging from 89.53 stomata mm‑2 (‘Nijisseiki’) up to 134.07 stomata mm‑2 (‘Housui’). SEM proved that Asian pear cultivars and ‘Kieffer Seedling’ shared ‘paracytic’ stomata type, while European pear cultivars had ‘anomocytic’ stomata type. Cluster analysis distinguished pear cultivars into two distinct groups, where European cultivars formed first sub-cluster and Asian together with ‘Kieffer Seedling’ second sub-cluster. Midrib traits and stomata type made a clear separation between the clusters. These results might suggest that thickness of midrib could be a huge barrier for Psylla sp. probing in Asian pears, thus representing one of the key factors in the resistance of these cultivars.

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The biosynthesis of polyphenolic compounds in cabbage waste, outer green leaves of white head cabbage (Brassica oleracea L. var. capitata subvar. alba), was stimulated by postharvest irradiation with UVB lamps or sunlight. Both treatments boosted the content of kaempferol and quercetin glycosides, especially in the basal leaf zone, as determined by the HPLC analysis of leaf extracts and by a non-destructive optical sensor. The destructive analysis of samples irradiated by the sun for 6 days at the end of October 2015 in Skierniewice (Poland) showed an increase of leaf flavonols by 82% with respect to controls. The treatment by a broadband UVB fluorescent lamp, with irradiance of 0.38 W m−2 in the 290–315 nm range (and 0.59 W m−2 in the UVA region) for 12 h per day at 17 °C along with a white light of about 20 μmol m−2 s−1, produced a flavonols increase of 58% with respect to controls. The kinetics of flavonols accumulation in response to the photochemical treatments was monitored with the FLAV non-destructive index. The initial FLAV rate under the sun was proportional to the daily radiation doses with a better correlation for the sun global irradiance (R2 = 0.973), followed by the UVA (R2 = 0.965) and UVB (R2 = 0.899) irradiance. The sunlight turned out to be more efficient than the UVB lamp in increasing the flavonols level of waste leaves, because of a significant role played by UVA and visible solar radiation in the regulation of the flavonoid accumulation in cabbage. The FLAV index increase induced on the adaxial leaf side was accompanied by a lower but still significant FLAV increase on the unirradiated abaxial side, likely due to a systemic signaling by mean of the long-distance movement of macromolecules. Our present investigation provides useful data for the optimization of postharvest photochemical protocols of cabbage waste valorization. It can represent a novel and alternative tool of vegetable waste management for the recovery of beneficial phytochemicals.

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Process-based grass models (PBGMs) are widely used for predicting grass growth under potential climate change and different management practices. However, accurate predictions using PBGMs heavily rely on field observations for data assimilation. In data-limited areas, performing robust and reliable estimates of grass growth remains a challenge. In this paper, we incorporated satellite-based MODIS data products, including leaf area index, gross primary production and evapotranspiration, as an additional supplement to field observations. Popular data assimilation methods, including Bayesian calibration and the updating method ensemble Kalman filter, were applied to assimilate satellite derived information into the BASic GRAssland model (BASGRA). A range of different combinations of data assimilating methods and data availability were tested across four grassland sites in Norway, Finland and Canada to assess the corresponding accuracy and make recommendations regarding suitable approaches to incorporate MODIS data. The results demonstrated that optimizing the model parameters that are specific for grass species and cultivar should be targeted prior to updating model state variables. The MODIS derived data products were capable of constraining model’s simulations on phenological development and biomass accumulation by parameter optimization with its performance exceeding model outputs driven by default parameters. By integrating even a small number of field measurements into the parameter calibration, the model’s predictive accuracy was further improved - especially at sites with obvious biases in the input MODIS data. Overall, this comparative study has provided flexible solutions with the potential to strengthen the capacity of PBGMs for grass growth estimation in practical applications.