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2025

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Research Highlights The microbial community applied with the digestate accelerated methane formation. CO2 emission was higher under waterlogging than at field capacity in soil with low organic content. Waterlogging decreased methane emission in unamended soil. Only digestate where some methane potential was left induced high methane emission. Methanogens and methanotrophs abundances dynamics partly explain emission patterns.

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Comparative studies between pasture-based dairy production and indoor confinement-based production have demonstrated that including pasture in the diet reduces enteric methane emissions. However, the effects of seasonal access to pasture, where animals are allowed outdoors only during parts of the year, have been less extensively studied. The aim of this study was to assess the effects of providing dairy cows with voluntary access to a production pasture compared with an exercise pasture on their performance and enteric methane emission. Thirty-two lactating Norwegian Red dairy cows, averaging 158 DIM, were divided into 2 groups and used in a changeover experimental design. The treatments included cows given 24-h access to either a production pasture (providing at least 50% of cows' daily DMI) with unlimited fresh grass and restricted indoor feeding, or ad libitum indoor feeding of silage with access to an exercise pasture with no expected herbage intake. Each changeover period included 2 wk of adaptation followed by 2 wk of recording, during which both treatments were fed the same grass silage and concentrates. Milk yield was recorded using an automatic milking unit, and indoor feed intake was recorded using access-controlled feed troughs and concentrate feeders. Enteric methane emissions were monitored using 2 GreenFeed emission monitoring systems: one positioned indoors, and one positioned outdoors adjacent to the production pasture. Cows on production pasture exhibited a lower milking frequency and reduced milk yield compared with cows on exercise pasture. However, cows on production pasture compensated for the lower milk yield by increased concentration of milk solids, suggesting that including a significant amount of pasture in the diet of dairy cows may not compromise energy-corrected milk production. Additionally, cows in the production pasture had significantly lower enteric methane emissions compared with cows in the exercise pasture. In conclusion, incorporating pasture into the diets of dairy cows, even if only for part of the year, has the potential to reduce the carbon footprint of dairy production.

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Humic acid (HA) is an inhibitor that can diminish bioconversion during anaerobic digestion (AD). In this study, a non-metallic nanomaterial, N-doped carbon quantum dots (NCQD), was synthesized to alleviate the HA inhibition, followed by exploring its mechanism. Adding 500 mg/L NCQD prevented HA inhibition by binding to HA or microorganism surfaces, thus avoiding contact between them. This phenomenon, known as shielding inhibition, involves the formation of hydrogen bonds and chemical bonds. The intermolecular force and dissociation constant (KD) between NCQD and HA were determined to be 112.83 ±3.98 nN and 1.0 ±0.07 ×10 7 M, respectively. NCQD could promote electron transfer in AD systems. According to the in-depth microbial analysis, NCQD significantly enhanced the metabolic pathways of methanogenesis and biosynthesis of coenzyme F420. This groundbreaking research demonstrates the dual functionality of NCQD by effectively shielding against HA inhibition and promoting electron transfer. The study also unravels the mechanism of interface interaction, electron transfer, and metabolic pathways, leading to significant breakthroughs in addressing HA inhibition.