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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2025

2024

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Sammendrag

Norway spruce (Picea abies (L.) H. Karst.) is a beneficial conifer species in Europe, especially in forestry. The increasing demand of Norway spruce has led to high production of seedlings in growth facilities. Growth conditions in these facilities contribute to fungal outbreaks, and the continuous use of fungicides to combat fungal infections will eventually develop resistance in the fungi. Applying chemical compounds directly to the seeds to prime inducible defences may be a simpler and safer method to protect the seedlings. In this study, seeds were treated with the chemicals methyl jasmonate (MeJA) and chitosan (Chi) to determine if seed priming is possible in Norway spruce. Furthermore, seeds were also treated with JAR, and a combination of JAR and MeJA or Chi, to investigate the hormone signalling pathways involved in MeJA and Chi induced defence responses. To establish the effects of these chemicals, the germination percentage and phenotypes of 4-week-old seedlings were evaluated. Additionally, 4-week-old seedlings were challenged with mechanical wounding and MeJA to determine if the seed treatments had an influence on defence responses. Gene expression levels of seven defence-related genes (CHI4, CHI2, NRPE1, ROS1, JAR1, LAR3, LOX) were quantified at two time points after challenge. In addition, chitinase enzyme activity was measured. The findings of this study indicates that the chemicals MeJA and Chi can possibly penetrate the seed coats of Norway spruce, making seed priming possible. However, this study did not include all defence responses and most genes did not reveal any priming effect, thus it was difficult to determine the signalling pathways involved in defence responses. Overall, the ontogeny of Norway spruce may play a major role in the activation of various defence mechanisms.