Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2023
Forfattere
Paulina Paluchowska Zhimin Yin Erik Lysøe Simeon Rossmann Mirella Ludwiczewska Marta Janiszewska Sylwester Sobkowiak Håvard Eikemo Monica Skogen May Bente Brurberg Jadwiga ŚliwkaSammendrag
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Forfattere
Carl Gunnar Fossdal Igor A. Yakovlev Marcos Viejo Paul Grini Paal Krokene Yupeng Zhang Jorunn Elisabeth OlsenSammendrag
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Forfattere
Yupeng Zhang Tuomas Toivainen Kathryn Mackenzie Igor A. Yakovlev Paal Krokene Timo Hytönen Paul Grini Carl Gunnar FossdalSammendrag
Temperature conditions experienced during embryogenesis and seed development may induce epigenetic changes that increase phenotypic variation in plants. Here we investigate if embryogenesis and seed development at two different temperatures (28 vs. 18°C) result in lasting phenotypic effects and DNA methylation changes in woodland strawberry (Fragaria vesca). Using five European ecotypes from Spain (ES12), Iceland (ICE2), Italy (IT4), and Norway (NOR2 and NOR29), we found statistically significant differences between plants from seeds produced at 18 or 28°C in three of four phenotypic features investigated under common garden conditions. This indicates the establishment of a temperature-induced epigenetic memory-like response during embryogenesis and seed development. The memory effect was significant in two ecotypes: in NOR2 flowering time, number of growth points and petiole length were affected, and in ES12 number of growth points was affected. This indicates that genetic differences between ecotypes in their epigenetic machinery, or other allelic differences, impact this type of plasticity. We observed statistically significant differences between ecotypes in DNA methylation marks in repetitive elements, pseudogenes, and genic elements. Leaf transcriptomes were also affected by embryonic temperature in an ecotype-specific manner. Although we observed significant and lasting phenotypic change in at least some ecotypes, there was considerable variation in DNA methylation between individual plants within each temperature treatment. This within-treatment variability in DNA methylation marks in F. vesca progeny may partly be a result of allelic redistribution from recombination during meiosis and subsequent epigenetic reprogramming during embryogenesis.
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Forfattere
Hang Su Andre van Eerde Espen Rimstad Ralph Bock Norica Branza-Nichita Igor A. Yakovlev Jihong Liu ClarkeSammendrag
Plants provide not only food and feed, but also herbal medicines and various raw materials for industry. Moreover, plants can be green factories producing high value bioproducts such as biopharmaceuticals and vaccines. Advantages of plant-based production platforms include easy scale-up, cost effectiveness, and high safety as plants are not hosts for human and animal pathogens. Plant cells perform many post-translational modifications that are present in humans and animals and can be essential for biological activity of produced recombinant proteins. Stimulated by progress in plant transformation technologies, substantial efforts have been made in both the public and the private sectors to develop plant-based vaccine production platforms. Recent promising examples include plant-made vaccines against COVID-19 and Ebola. The COVIFENZ® COVID-19 vaccine produced in Nicotiana benthamiana has been approved in Canada, and several plant-made influenza vaccines have undergone clinical trials. In this review, we discuss the status of vaccine production in plants and the state of the art in downstream processing according to good manufacturing practice (GMP). We discuss different production approaches, including stable transgenic plants and transient expression technologies, and review selected applications in the area of human and veterinary vaccines. We also highlight specific challenges associated with viral vaccine production for different target organisms, including lower vertebrates (e.g., farmed fish), and discuss future perspectives for the field.
Forfattere
Samuel W. Wilkinson Adam Hannan Parker Amos Muench Robert Simon Wilson K. Hooshmand M.A. Henderson Emma K. Moffat P.S.C.F. Rocha H. Hipperson J.H.M. Stassen Ana López Sánchez Inge S. Fomsgaard Paal Krokene Melissa Magerøy Jurriaan TonSammendrag
Stress can have long-lasting impacts on plants. Here we report the long-term effects of the stress hormone jasmonic acid (JA) on the defence phenotype, transcriptome and DNA methylome of Arabidopsis. Three weeks after transient JA signalling, 5-week-old plants retained induced resistance (IR) against herbivory but showed increased susceptibility to pathogens. Transcriptome analysis revealed long-term priming and/or upregulation of JA-dependent defence genes but repression of ethylene- and salicylic acid-dependent genes. Long-term JA-IR was associated with shifts in glucosinolate composition and required MYC2/3/4 transcription factors, RNA-directed DNA methylation, the DNA demethylase ROS1 and the small RNA (sRNA)-binding protein AGO1. Although methylome analysis did not reveal consistent changes in DNA methylation near MYC2/3/4-controlled genes, JA-treated plants were specifically enriched with hypomethylated ATREP2 transposable elements (TEs). Epigenomic characterization of mutants and transgenic lines revealed that ATREP2 TEs are regulated by RdDM and ROS1 and produce 21 nt sRNAs that bind to nuclear AGO1. Since ATREP2 TEs are enriched with sequences from IR-related defence genes, our results suggest that AGO1-associated sRNAs from hypomethylated ATREP2 TEs trans-regulate long-lasting memory of JA-dependent immunity.
Forfattere
Marcos Viejo Manuel Rey Yolanda Ferradas Javier Sampedro Jorunn Elisabeth Olsen Carl Gunnar Fossdal Igor A. Yakovlev Ana Lucia Pinto-Sintra Maria Victoria GonzalesSammendrag
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Purpose of Review Forestry in northern temperate and boreal regions relies heavily on conifers. Rapid climate change and associated increases in adverse growing conditions predispose conifers to pathogens and pests. The much longer generation time and presumably, therefore, lower adaptive capacity of conifers relative to their native or non-native biotic stressors may have devastating consequences. We provide an updated overview of conifer defences underlying pathogen and pest resistance and discuss how defence traits can be used in tree breeding and forest management to improve resistance. Recent Findings Breeding of more resilient and stress-resistant trees will benefit from new genomic tools, such as genotyping arrays with increased genomic coverage, which will aid in genomic and relationship-based selection strategies. However, to successfully increase the resilience of conifer forests, improved genetic materials from breeding programs must be combined with more flexible and site-specific adaptive forest management. Summary Successful breeding programs to improve conifer resistance to pathogens and pests provide hope as well as valuable lessons: with a coordinated and sustained effort, increased resistance can be achieved. However, mechanisms underlying resistance against one stressor, even if involving many genes, may not provide any protection against other sympatric stressors. To maintain the adaptive capacity of conifer forests, it is important to keep high genetic diversity in the tree breeding programs. Choosing forest management options that include diversification of tree-species and forest structure and are coupled with the use of genetically improved plants and assisted migration is a proactive measure to increase forest resistance and resilience to foreseen and unanticipated biotic stressors in a changing climate.
Sammendrag
Strawberry is a high-value commercial crop and a model for the economically important Rosaceae family. Strawberry is vulnerable to attack by many pathogens that can affect different parts of the plant, including the shoot, root, flowers, and berries. To restrict pathogen growth, strawberry produce a repertoire of secondary metabolites that have an important role in defense against diseases. Terpenes, allergen-like pathogenesis-related proteins, and flavonoids are three of the most important metabolites involved in strawberry defense. Genes involved in the biosynthesis of secondary metabolites are induced upon pathogen attack in strawberry, suggesting their transcriptional activation leads to a higher accumulation of the final compounds. The production of secondary metabolites is also influenced by the beneficial microbes associated with the plant and its environmental factors. Given the importance of the secondary metabolite pathways in strawberry defense, we provide a comprehensive overview of their literature and their role in the defense responses of strawberry. We focus on terpenoids, allergens, and flavonoids, and discuss their involvement in the strawberry microbiome in the context of defense responses. We discuss how the biosynthetic genes of these metabolites could be potential targets for gene editing through CRISPR-Cas9 techniques for strawberry crop improvement.