Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2022
Forfattere
Daniel RasseSammendrag
Det er ikke registrert sammendrag
Forfattere
Daniel RasseSammendrag
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Forfattere
Daniel RasseSammendrag
Det er ikke registrert sammendrag
Forfattere
Daniel RasseSammendrag
Det er ikke registrert sammendrag
Forfattere
Ana-Maria Pantazica Mihaela-Olivia Dobrica Catalin Lazar Cristina Scurtu Catalin Tucureanu Iuliana Caras Irina Ionescu Adriana Costache Adrian Onu Jihong Liu Clarke Crina Stavaru Norica Branza-NichitaSammendrag
Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines.
Forfattere
Jihong Liu ClarkeSammendrag
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Forfattere
Shu Zhang Lingbo Meng Jian Hou Xiaodan Liu Abiola O. Ogundeji Zeyu Cheng Tengjiao Yin Nicholas Clarke Baozhong Hu Shumin LiSammendrag
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Forfattere
Daniel Liptzin Jens Boy John L. Campbell Nicholas Clarke Jean-Paul Laclau Roberto Godoy Sherri L. Johnson Klaus Kaiser Gene E. Likens Gunilla Pihl Karlsson Daniel Markewitz Michela Rogora Stephen D. Sebestyen James B. Shanley Elena Vanguelova Arne Verstraeten Wolfgang Wilcke Fred Worrall William H. McDowellSammendrag
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Forfattere
Inge Stupak Nicholas Clarke Andis Lazdins Iveta Kabasinskiene Diana Lukmine Dagnija LazdinaSammendrag
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Sammendrag
Heat Field Deformation (HFD) is a widely used method to measure sap flow of trees based on empirical relationships between heat transfer within tree stems and the sap flow rates. As an alternative, the Linear Heat Balance (LHB) method implements the same instrumental configuration as HFD but calculates the sap flow rates using analytical equations that are derived from fundamental conduction-convection heat transfer theories. In this study, we systematically compared the sap flow calculated using the two methods based on data that were recorded using the same instrument. The measurements were conducted on four Norway spruce trees. We aimed to evaluate the discrepancies between the sap flow estimates from the two methods and determine the underlying causes. Diurnal and day-to-day patterns were consistent between the sap flow estimates from the two methods. However, the magnitudes of the estimated sap flow were different between them, where LHB resulted in much lower estimates in three trees and slightly higher estimates in one compared to HFD. We also observed larger discrepancies in negative (reversed flow) than in positive sap flow, where the LHB resulted in lower reversed flow than HFD. Consequently, the seasonal budget estimated by LHB can be as low as ∼20% of that estimated by HFD. The discrepancies can be mainly attributed to the low wood thermal conductivities for the studied trees that lead to substantial underestimations using the LHB method. In addition, the sap flow estimates were very sensitive to the value changes of the empirical parameters in the calculations and, thus, using a proper case-specific value is recommended, especially for the LHB method. Overall, we suggest that, despite the strong theoretical support, the correctness of LHB outputs depends largely on the tree individuals and should be carefully evaluated.