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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2017

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Helminthosporium solani causes silver scurf, which affects the quality of potato. The biocontrol agent Clonostachys rosea greatly limited the severity of silver scurf symptoms and amount of H. solani genomic DNA in laboratory experiments. Transcriptomic analysis during interaction showed that H. solani gene expression was highly reduced when coinoculated with the biocontrol agent C. rosea, whereas gene expression of C. rosea was clearly boosted as a response to the pathogen. The most notable upregulated C. rosea genes were those encoding proteins involved in cellular response to oxidative stress, proteases, G-protein signaling, and the methyltransferase LaeA. The most notable potato response to both fungi was downregulation of defense-related genes and mitogen-activated protein kinase kinase kinases. At a later stage, this shifted, and most potato defense genes were turned on, especially those involved in terpenoid biosynthesis when H. solani was present. Some biocontrol-activated defense-related genes in potato were upregulated during early interaction with C. rosea alone that were not triggered by H. solani alone. Our results indicate that the reductions of silver scurf using C. rosea are probably due to a combination of mechanisms, including mycoparasitism, biocontrol-activated stimulation of plant defense mechanisms, microbial competition for nutrients, space, and antibiosis.

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Green roofs are used increasingly to alleviate peaks of water discharge into the sewage systems in urban areas. Surface runoff from roofs contain pollutants from dry and wet deposition, and green roofs offer a possibility to reduce the amounts of pollutants in the water discharged from roofs by degradation and filtering. These pollutants would otherwise enter wastewater treatments plants and ultimately end up in sewage sludge that is spread on agricultural soils. The most common substrates used in green roofs have limited capacity for filtration and sorption. Also, more sustainable alternatives are sought, due to the high carbon footprint of these materials. Biochar is a carbon-rich material produced by pyrolysis of biomass, and several types of biochar have been described as good sorbents and filter materials. Biochar is also a light and carbon negative material, which may fulfill other desired criteria for new green roof substrates. We here report on an experiment where two types of biochar, produced from olive husks at 450 °C or from forest waste at 850 ° C were mixed with volcanic rock or peat, and tested for retention capacity of phenanthrene and six heavy metals in a column experiment with unsaturated gravimetric water flow lasting for 3 weeks. The results suggest that biochar as a component in green roof substrates perform better than traditional materials, concerning retention of the tested pollutants, and that different types of biochar have different properties in this respect.

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This work presents the outcomes from two independent studies evaluating the chemical quality of groundwater in agricultural areas irrigated with wastewater from sugar and yeast industries. The evaluation was determined using chemical parameters representing typical contaminants of sugar industry wastewater (SIWW) and yeast industry wastewater (YIWW), and characterising the content of organic matter (BOD5), nutrients (NH4-N, NO3-N, TN and TP) and salts (Cl, SO4, Na and K). The studies reveal that food industry wastewater constitutes a valuable water-nutrient-rich medium that can be reused in agricultural applications as an alternative water resource for irrigation and nutrients for fertilisation. Furthermore, the reuse facilitates the sustainable discharge of wastewater through a soil-aquifer zone to the natural environment. This does not affect chemical quality of groundwater, which was comparable in areas irrigated and non-irrigated with SIWW and YIWW. Although some parameters (NO3-N, NH4-N, SO4, Cl and Na) displayed higher concentrations in groundwater from the fields irrigated with wastewater, these contents were within recommended healthbased guideline limits defined in either the groundwater quality standards or the drinking water quality norms. Only the contents of K revealed an exclusive groundwater impact from wastewater irrigation. This was confirmed in statistical tests employing theWard’s hierarchical clustering method, which exposed excessive amounts of K introduced into groundwater through irrigation with both SIWW and YIWW. However, this parameter is not considered to pose any health risk to humans or the environment, and its content is not restricted by quality guideline values for either groundwater or drinking water.

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Ungulate browsing results in important damages on the forests, affecting their structure, composition and development. In the present paper, we examine the occurrence of browsing damage in Norwegian forests, using data provided by the National Forest Inventory along several consecutive measurements (entailing the period 1995–2014). A portfolio of variables describing the stand, site and silvicultural treatments are analyzed using classification trees to retrieve combinations related to browsing damage. Our results indicate that the most vulnerable forest stands are young with densities below 1400 trees ha–1 and dominated by birch, pine or mixed species. In addition, stand diversity and previous treatments (e.g. thinnings) increase the damage occurrence and other variables, like stand size, could play a role on forest susceptibility to browsing occurrence although the latter is based on weaker evidence. The methods and results of our study can be applied to implement management measures aiming at reducing the browsing damages of forests.

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Consumer resistance against GM crops is still substantial in the United States and Europe. We conducted an internet survey in the United States and Norway with more than 1,000 respondents in each country to estimate consumers’ willingness to pay (WTP) for GM soybean oil, farmed salmon fed with GM soy, and GM salmon. The differences in WTP for the conventional as compared with the GM alternatives are relatively small. Only between 7 and 13% of the respondents indicated that they were willing to pay more than a 20% premium for each of the conventional alternatives as compared to the corresponding GM alternatives. The average WTP premiums range from 7.5 to 9.2%. This suggests a large similarity in WTP in Norway and the United States and across the three products.

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Acetophenones are phenolic compounds involved in the resistance of white spruce (Picea glauca) against spruce budworm (Choristoneura fumiferiana), a major forest pest in North America. The acetophenones pungenol and piceol commonly accumulate in spruce foliage in the form of the corresponding glycosides, pungenin and picein. These glycosides appear to be inactive against the insect but can be cleaved by a spruce b-glucosidase, PgbGLU-1, which releases the active aglycons. The reverse glycosylation reaction was hypothesized to involve a family 1 UDP-sugar dependent glycosyltransferase (UGT) to facilitate acetophenone accumulation in the plant. Metabolite and transcriptome profiling over a developmental time course of white spruce bud burst and shoot growth revealed two UGTs, PgUGT5 and PgUGT5b, that glycosylate pungenol. Recombinant PgUGT5b enzyme produced mostly pungenin, while PgUGT5 produced mostly isopungenin. Both UGTs also were active in vitro on select flavonoids. However, the context of transcript and metabolite accumulation did not support a biological role in flavonoid metabolism but correlated with the formation of pungenin in growing shoots. Transcript levels of PgUGT5b were higher than those of PgUGT5 in needles across different genotypes of white spruce. These results support a role of PgUGT5b in the biosynthesis of the glycosylated acetophenone pungenin in white spruce.