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2022

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One part of aquaculture sustainability is reducing the environmental footprint of aquaculture feeds. For European aquaculture, this means finding feed ingredients that are produced within the economic community, and that are not in conflict with human consumption. This is especially challenging when formulating diets for carnivorous fish such as turbot with low tolerance to fishmeal replacement that are both nutritious and economically and environmentally sustainable. Therefore, we investigated the effects of two novel and innovative feed formulation concepts on growth and feed performance and the nutritional status of market-sized turbot in a recirculating aquaculture system. In a 16-week feeding trial, 440 turbot (300 ± 9 g) were fed twice a day with a control diet (CTRL), based on a commercial formulation, and four experimental diets. The experimental diets were designed to investigate the effects of two formulations concepts based on sustainable terrestrial plant proteins (NoPAP) or processed animal proteins (PAP) and of 30% and 60% fishmeal replacement with emerging feed ingredients (fisheries by-products, insect meal and fermentation biomass). Turbot from the CTRL group had a similar growth and feed performance than fish fed the NoPAP30 formulation, with a significant decline of performance in the fish fed both PAP formulations and the NoPAP60. Comparing the two formulation concepts with each other the voluntary feed intake and protein efficiency ratio on tank basis as well as the individual weight gain and relative growth rate was significantly higher in the fish from the NoPAP groups than PAP groups. Furthermore, the apparent digestibility of nutrients and minerals was significantly reduced in the fish fed with the diets with 30% and 60% fishmeal replacement level compared to the fish from the CTRL group. In conclusion, the performance of the fish fed the NoPAP30 formulation concept highlights the potential of the used combination of sustainable ingredients, such as fisheries by-products, insect meal, microbial biomass and plant protein for turbot. Furthermore, this study shows that turbot has a higher tolerance to the incorporation of plant and insect protein than of processed animal protein.

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Fishbones contain significant amounts of plant nutrients. Fish residues may be preserved by acidification to pH < 4, which may affect the chemical extractability, and the plant availability of nutrients when applied as fertilisers. Grinded bone material from cod (Gadus morhua) heads was mixed with formic acid to investigate if this would increase the concentrations of ammonium lactate–acetate (AL)-extractable nutrients. Two degrees of fineness of fishbones (coarse 2–4 mm; fine < 0.71 mm) were compared at pH 3.0 and 4.0 plus a water control in a laboratory study over 55 days. Samples for measurement of AL-extractable P, Ca, Mg and K were taken on day 2, 15, 34 and 55. Whereas more formic acid and thereby lower pH clearly increased the concentrations of AL-extractable calcium (Ca-AL) and magnesium (Mg-AL), AL-extractable phosphorus (P-AL) was only significantly increased in finely grinded bones at pH 3. After 34 days at pH 3, 6% of the total content of P was extracted by AL in fine fishbones. In the water control, about 1% of the P was extracted, possibly from phospholipids. This P-AL concentration was well above P-AL extracted from acidified coarse fishbones (pH 3 and 4) and from fine fishbones acidified to pH 4. With acidification, about 30% of total Ca and 100% of total Mg were extracted by AL, and the Ca-AL and Mg-AL concentrations were closely correlated. A possible reason for lower P-AL in coarse fishbones at pH 3 and 4, and in fine fishbones at pH 4 than in water controls may be a precipitation of apatite from phospholipids and dissolved calcium.

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Semi- and nonparametric models are popular in the area-based approach (ABA) using airborne laser scanning. It is unclear, however, how many predictors and training plots are needed to provide accurate predictions without overfitting. This work aims to explore these limits for various approaches: ordinary least squares regression (OLS), generalized additive models (GAM), least absolute shrinkage and selection operator (LASSO), random forest (RF), support vector machine (SVM), and Gaussian process regression (GPR). We modeled timber volume (m3·ha–1) for four boreal sites using ABA with 2–39 predictors and 20–500 training plots. OLS, GAM, LASSO, and SVM overfitted as the number of predictors approached the number of training plots. They required ≥15 plots per predictor to provide accurate predictions (RMSE ≤30%). GAM required ≥250 plots regardless of the number of predictors. The number of predictors only mildly affected RF and GPR, but they required ≥200 and ≥250 training plots, respectively. RF did not overfit in any circumstances, whereas GPR overfit even with 500 training plots. Overall, using up to 39 predictors did not generally result in overfit, and for most model types, it resulted in better accuracy for sufficiently large datasets (≥250 plots).

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Biosynthesis of phytochemicals in leaves of Brassica can be initiated by abiotic factors. The aim of the study was to investigate elicitor treatments to add value to waste of cabbage. A leaf waste fraction from industrial trimming of head cabbage was exposed to UV radiation (250–400 nm, 59 and 99 kJ∙m−2, respectively), photosynthetic active radiation (PAR, 400–700 nm, 497 kJ∙m−2), and ultrasound in water bath (35 kHz, at 15, 30 and 61 kJ∙l−1 water), in order to improve nutraceutical concentration. UV was more effective than PAR to increase the level of flavonols (2 to 3-fold higher) and hydroxycinnamate monosaccharides (1 to 10-fold higher). PAR was three times as effective as UV to increase anthocyanins. Interaction of PAR + UV increased antioxidant activity (30%), the content of five phenolics (1.4 to 10-fold higher), and hydroxycinnamic monosaccharides (compared with PAR or UV alone). Indoles were reduced (40–52%) by UV, but the other glucosinolates (GLS) were unaffected. Ultrasound did not influence any parameters. The results are important for white cabbage by-products by demonstrating that UV + PAR can be successfully used as an effectual tool to increase important phenolics and antioxidant activity of waste fraction leaves without an adverse effect on the main GLS.

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The plant pomological characteristics and physiological behaviors of genotypes in modern apple cultivation could be different depending on the use of rootstock, changing growth ecology and application of biological control agents. The aim of this research was to determine the effects of rhizobacteria application on leaf and fruit nutrient contents in different apple scion–rootstock combinations. This study was carried out with seven standard cultivars (Scarlet Spur, Red Chief, Fuji, Jeromine, Galaxy Gala, Granny Smith, and Golden Reinders) budded on M.9 and MM.106 rootstocks. In the experiment, trees were sprayed by a nitrogen + phosphorus solvent rhizobacteria three times, with an interval of 15 days in the spring period. The effect of rhizobacteria application on leaf and fruit nutrient contents was statistically significant and provided generally significant positive contributions, except for leaf Mg content. Comparing both rootstocks, the positive effect of bacterial application was higher on the M.9 rootstock for leaf N and B content and fruit N and Fe content, and on the MM.106 rootstock for other nutrient content. While the effects of bacterial application on the basis of cultivars were generally positive, the highest positive contribution was made in leaf P content (10.7%) and fruit Mn content (32.1%) of the Fuji cultivar. Considering the total increase in nutrients in scion–rootstocks combination, rhizobacteria application had a positive effect on the leaf nutrient contents in Golden Reinders/MM.106, but not leaf K content. The highest increases in leaves of scion-rootstock combinations were determined as 4.0% in N content in Granny Smith/M.9, 14.1% in P content in Scarlet Spur/MM.106, 7.1% in K content in Fuji/MM.106, 4.4% in Ca content in Jeromine/M.9, and 14.0% in Mg content in Granny Smith/MM.106. The highest increase in fruit nutrient contents was between 4.9% (N content) and 13.5% (Ca content) for macro elements, and between 9.5% (Cu content) and 41.8% (Mn content) for microelements. The results of the present study may provide significant leads for further studies on this subject.