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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2022

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The aim of this study was to examine the influence of shoot age on the biological and chemical properties of 13 black currant cultivars with different origins and ripening times. Phenological observations together with examined pomological and chemical characteristics were studied in two consecutive years at the experimental field near Belgrade, Serbia. The total content of phenols was estimated spectrophotometrically by the Folin-Ciocalteu method, while quantitative analysis of anthocyanin and flavonols aglycones was performed using a high-performance liquid chromatographic (HPLC) method. Principal component analysis was performed to establish differences in biological and chemical properties of black currants. Three-year-old shoots had an earlier start of all examined phenological stages, better generative potential, higher yields, while clusters and berries from 2-year-old shoots had significantly higher values for physical properties, total phenols, anthocyanin and flavanols aglycones and antiradical capacity. Late ripening cultivars had higher contents of all chemical compounds. The berries on 2-year-old shoots had total phenolics that ranged between 123.0 (‘Titania’) and 298.3 mg/100 g fresh weight (FW) (‘Ometa’), while total anthocyanins ranged between 398.5 (’Ojebyn’) and 1160.8 mg/kg FW (’Ometa’). According to the obtained results, cultivars ‘Ometa’, ‘Ben Lomond’, ‘Tsema’ and ‘Malling Juel’ can be recommended as the most promising for growing in the continental climate because they stood out with higher generative potential and yield, physical traits of cluster and berry, higher level of primary and secondary metabolites and DPPH activity in their berries.

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The Expert Group for Technical Advice on Organic Production (EGTOP) was requested to advise on the use of several substances in organic production. The Group discussed whether the use of these substances is in line with the objectives and principles of organic production and whether they should therefore be included in Annex III of Reg. (EU) 2021/1165. With respect to feed the Group recommends the following: - Leonardite should not be included in Annex III. - Sepiolitic clay should be included in Annex III, part B. - Peat should not be included in Annex III. With respect to pet food, the Group recommends the following: - Locust bean gum should be included in Annex III, part B with the following conditions/limits: only for pet food and obtained only from the roasting process and from organic production, if available. - Acacia-Arabic gum should be included in Annex III, part B with the following conditions/limits: only for pet food and from organic production, if available. - Carrageenan should be included in Annex III, part B with the following conditions/limits: only for pet food. - Ammonium chloride should be included in Annex III, part B with the following conditions/limits: only for pet food intended to be used for special nutritional purposes for cats. - (Ortho-)phosphoric acid should not be included in Annex III. - Taurine should be included in Annex III, part B with the following conditions/limits: only for cats and dogs, not from GMO origin and if possible not from synthetic origin. - Methionine should not be included in Annex III. - Disodium dihydrogen diphosphate (SAPP) should be included in Annex III, part A with the following conditions/limits: only for pet food. - Pentasodium triphosphate (STPP) should be included in Annex III, part A with the following conditions/limits: only for pet food.

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Ongoing efforts focus on quantifying plastic pollution and describing and estimating the related magnitude of exposure and impacts on human and environmental health. Data gathered during such work usually follows a receptor perspective. However, Life Cycle Assessment (LCA) represents an emitter perspective. This study examines existing data gathering and reporting approaches for field and laboratory studies on micro- and nanoplastics (MNPs) exposure and effects relevant to LCA data inputs. The outcomes indicate that receptor perspective approaches do not typically provide suitable or sufficiently harmonised data. Improved design is needed in the sampling, testing and recording of results using harmonised, validated and comparable methods, with more comprehensive reporting of relevant data. We propose a three-level set of requirements for data recording and reporting to increase the potential for LCA studies and models to utilise data gathered in receptor-oriented studies. We show for which purpose such data can be used as inputs to LCA, particularly in life cycle impact assessment (LCIA) methods. Implementing these requirements will facilitate proper integration of the potential environmental impacts of plastic losses from human activity (e.g. litter) into LCA. Then, the impacts of plastic emissions can eventually be connected and compared with other environmental issues related to anthropogenic activities.

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Many wild animals perceive humans as predators, and human disturbance,especially in the form of hunting, triggers antipredatory behavior among prey.Yet, knowledge of how game species react to different types of human distur-bance and adapt to repeated disturbances is limited. We investigated howdisturbance in the form of a solitary human approacher (stalker) impactedbehavior (flight response and short-term habitat use) of 28 GPS-collared reddeer (Cervus elaphus) in two populations with contrasting population densitiesin Norway. We studied how the behavioral response differed: (1) with season(pre-hunting vs. hunting); (2) by consecutive approaches within a day;(3) among replicated experiments within the same season; and (4) betweentwo regions with contrasting densities of red deer. The average flight initiationdistance (FID) increased by 15% during the hunting season, and consecutiveapproaches within the same day caused the red deer to move 49% longerdistances. Flight initiation distance was longer in the high-density population,while escape distance was longer in the low-density population. Red deermoved out of their weekly home range after 52% approaches, and after theonset of hunting season, time spent outside the home range increased by 89%.Red deer preferred denser resting sites after the disturbance and animal siteshad shorter sighting distance and higher canopy cover than control plots.Tree density and canopy cover at animal sites increased at the onset of huntingseason, from first to second approach within day, and after replicated experi-ments within season. Our results suggest that red deer preferred dense restingsites, especially in the hunting season. However, these animal sites had thesame amount of the favorable forage plant bilberry (Vaccinium myrtillus), indi-cating no clear food–cover trade-off in selection of habitat. Our study showedthat onset of hunting initiates stronger fear responses in red deer, which mayin turn affect red deer distribution and harvesting efficiency

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The success of the mollusc-parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) Andrássy (Rhabditida: Rhabditidae), as a biological control agent in Europe has led to worldwide interest in phasmarhabditids as biocontrol agents. In this study, the mass culture potential of three phasmarhabditids, namely Phasmarhabditis papillosa, Phasmarhabditis kenyaensis and Phasmarhabditis bohemica, was assessed. In addition, ten bacterial candidates, consisting of seven associated with slugs and three associated with entomopathogenic nematodes, were investigated. The bacteria were tested for their ability to cause mortality to Deroceras invadens, as well as to support nematode growth. Initial mortality studies demonstrated that Kluyvera, Aeromonas and Pseudomonas spp. (AP3) caused 100% mortality when they were injected into the haemocoel of D. invadens. However, in growth studies, Pseudomonas sp. (AP4) was found to be the most successful bacterium, leading to recovery and reproduction in almost all nematode species, except for P. kenyaensis. In flask studies, P. bohemica, which showed exceptional growth with Pseudomonas sp. (AP1), was chosen for further investigation. The effect of inoculating flasks with different concentrations of Pseudomonas sp. (AP1), as well as with different concentrations of P. bohemica, was evaluated by assessing the nematode populations for 14 days. The results indicated that the lowest, 1% (v/v), bacteria inoculation led to higher total nematode and to infective juvenile (IJ) yield, with flasks with the highest IJ inoculum (3000 IJs/ml) having a positive effect on the total number of nematodes and IJs in cultures of P. bohemica. This study presents improvements for the mass-culturing of nematodes associated with molluscs.

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Sustainable phosphorus use is essential in golf course management to prevent eutrophication and overconsumption. The study aimed to investigate if phosphorus fertilization can be reduced without negative effects on turf quality. We compared two P fertilization recommendations based on soil analyses, one based on the annual nitrogen rate, and a zero-P control. The recommendations were the “minimum level of sustainable nutrition” (MLSN), which aims to keep treatment soil levels above 18 mg P kg–1 dry soil (Mehlich-3); the “sufficiency level of available nutrition” (SLAN), in which the threshold for excluding P fertilization is >54 mg P kg–1 dry soil (Mehlich-3); and “Scandinavian precision fertilization” (SPF), which recommends applying P at 12% of the annual N rate. The treatments were compared via monthly assessments of turf quality and the coverage of sown species and annual bluegrass (Poa annua L.) from 2017 to 2020 on five golf courses from Germany, Sweden, China, Norway, and the Netherlands. MLSN and SPF significantly reduced soil P at all sites compared with SLAN recommendations. Turf quality showed no significant differences. The results from the mixed creeping bentgrass (Agrostis stolonifera L.)–annual bluegrass green showed a 2 to 4% increase in annual bluegrass coverage with P fertilization over the zero-P treatments. The MLSN guideline is recommended for sustainable P fertilization on established greens with low P sorption capacity under diverse climatic and management conditions. The SPF may result in application of excess P to soils with high Mehlich-3 values, as soil analyses are not considered.

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Denne rapporten presenterer datagrunnlag for matsvinn og kosthold og mulighetene for å beregne hvilken effekt redusert matsvinn og endret kosthold vil ha på klimagassutslipp fra det norske jordbruket. Redusert matsvinn og endret kosthold vil endre etterspørselen etter mat, som vil påvirke jordbruksproduksjonen og på den måten også påvirke størrelsen på klimagassutslippet fra jordbruket. Endret etterspørsel etter mat inngår som del B i klimaavtalen mellom jordbruket og regjeringen, og det er ønskelig å utvikle en metode som kan skille effekten av endret etterspørsel etter mat, fra effekten av andre tiltak i jordbruket, på observerte endringer i klimagassutslipp. Muligheten for å følge utvikling i reduksjon av matsvinn er god, takket være Bransjeavtalen for matsvinn, som inkluderer innsamling av data og bearbeiding av disse. Matforsyningsstatistikken er en årlig framstilling av de mengdene matvarer som er tilgjengelig for det norske markedet, både norskprodusert og importert. Kombinert gjør disse datagrunnlagene det mulig å følge hvordan utviklingen i både matsvinn og kosthold, og hvordan endringer påvirker etterspørsel av norskprodusert mat. Måletidspunkt for matsvinn i primærleddet gjør at det er hensiktsmessig at det ikke inngår som en del av matsvinn i resten av verdikjeden for mat. Matsvinn på primærleddet måles som andel av totalt høstet/slaktet/plukket matvare, som gjør det enkelt å koble til jordbruksproduksjon. Målet om redusert klimagassutslipp tilsvarende 5 millioner tonn CO2-ekvivalenter i Klimaavtalen, skal måles i forhold til referansebanen for utslipp som ble laget til Nasjonalbudsjettet 2019. Referansebanen er basert blant annet på framskrivninger av dyretall, som igjen bygger på forventet befolkningsframskriving og forventninger til etterspørsel av melk, kjøtt og egg. Framskrivning av dyretall gir dermed også framskrivning av etterspørsel etter melk, egg og kjøtt, som kan sammenlignes med observert etterspørsel. For andre jordbruksprodukter som korn, bær og poteter, er det ikke like enkelt å lage framskrivning for etterspørsel som samsvarer med referansebanen for utslipp...

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Milder winters and extended wetter periods in spring and autumn limit the amount of time available for carrying out ground-based forest operations on soils with satisfactory bearing capacity. Thus, damage to soil in form of compaction and displacement is reported to be becoming more widespread. The prediction of trafficability has become one of the most central issues in planning of mechanized harvesting operations. The work presented looks at methods to model field measured spatio-temporal variations of soil moisture content (SMC, [%vol]) – a crucial factor for soil strength and thus trafficability. We incorporated large-scaled maps of soil characteristics, high-resolution topographic information – depth-to-water (DTW) and topographic wetness index – and openly available temporal soil moisture retrievals provided by the NASA Soil Moisture Active Passive mission. Time-series measurements of SMC were captured at six study sites across Europe. These data were then used to develop linear models, a generalized additive model, and the machine learning algorithms Random Forest (RF) and eXtreme Gradient Boosting (XGB). The models were trained on a randomly selected 10% subset of the dataset. Predictions of SMC made with RF and XGB attained the highest R2 values of 0.49 and 0.51, respectively, calculated on the remaining 90% test set. This corresponds to a major increase in predictive performance, compared to basic DTW maps (R2 = 0.022). Accordingly, the quality for predicting wet soils was increased by 49% when XGB was applied (Matthews correlation coefficient = 0.45). We demonstrated how open access data can be used to clearly improve the prediction of SMC and enable adequate trafficability mappings with high spatial and temporal resolution. Spatio-temporal modelling could contribute to sustainable forest management.

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Cuticle is the first layer protecting plants against external biotic and abiotic factors and is responsive to climatic factors as well as determined by genetic adaptations. In this study, the chemical composition of bilberry fruit cuticular wax was investigated through a latitudinal gradient from Latvia (56°N 24°E) through Finland (65°N 25°E) to northern Norway (69°N 18°E) in two seasons 2018 and 2019. Changes in the major cuticular wax compounds, including triterpenoids, fatty acids, alkanes, aldehydes, ketones, and primary alcohols, were detected by GC-MS analysis. Generally, a decreasing trend in the proportion of triterpenoids from southern to northern latitudes, accompanied with an increase in proportion of fatty acids, aldehydes, and alkanes, in bilberry fruit cuticular wax was observed. A correlation analysis between climatic factors with proportion of wax compounds indicated that temperature was the main factor affecting the cuticular wax composition in bilberries. A controlled phytotron experiment with southern and northern bilberry ecotypes confirmed the major effect of temperature on bilberry fruit cuticular wax load and composition. Elevated temperature increased wax load most in berries of northern ecotypes. The level of triterpenoids was higher, while levels of fatty acids and alkanes were lower, in wax of bilberry fruits ripened at 18°C compared to 12°C in both northern and southern ecotypes. Based on our results, it can be postulated that the predicted increase in temperature due to climate change leads to alterations in fruit cuticular wax load and composition. In northern ecotypes, the alterations were especially evident.

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Key words: VKM, risk assessment, Norwegian Scientific Committee for Food and Environment, Norwegian Environment Agency, potential toxic elements (PTEs), fertiliser, soil improver, fertiliser products, growing media, circular economy, circulation of organic fertilisers, arsenic (As), cadmium (Cd), chromium Cr(tot) (Cr(III) and Cr(VI)), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), zinc (Zn). Background and purpose of the report The potentially toxic elements (PTE) arsenic (As), cadmium (Cd), chromium Cr(tot) (Cr(III) and Cr(VI)), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni) and zinc (Zn) occur as ingredients or contaminants in many fertilisers, soil improvers, engineered soil and growing media. Application of these fertiliser products might represent a risk towards the environment, farm animals and humans, particularly when applied annually over several years. The present risk assessment evaluates the application of selected fertilisers according to certain scenarios for representative Norwegian agricultural areas, from Troms in the North to Ås in Southeastern and Time in Southwestern Norway, with different soil properties, precipitation and PTE concentration in present agricultural soil. There is an increasing trend to produce locally (e.g. in urban farming) and home-grown vegetables that are cultivated in engineered soil and growth media. The maximum levels (MLs) set for PTEs in different organic fertilisers, engineered soil and growing media for use in urban farming, home growing and the cultivation of vegetables and garden fruits, and a set of MLs also for application in agricultural cultivation of crops, have been evaluated. Environmental fate processes and the transfer of PTEs have been modelled and the environmental risks for terrestrial and aquatic organisms, including from secondary poisoning have been estimated. Potential risks to humans and farmed animals by increased exposure to PTEs from, respectively, agriculturally produced crops, vegetables cultivated at home and urban farming or forage and grazing have been evaluated. The recycling of nutrients is urgently needed to achieve circular economy, but the derived sustainable products have to be safe, which requires the introduction of and adherence to science-based maximum levels of unwanted substances (e.g. pollutants). This assessment evaluates consequences of the application of different fertiliser products: mineral P fertilisers, manure from cattle, pig, poultry and horse, fish sludge, digestates and sewage sludge - in order to identify PTE sources with potential environmental, animal and human health risks, and to evaluate the appropriateness of the current MLs regarding different applications of organic-based fertilisers, engineered soil and growing media at present, and in a 100-year perspective. Approach and methods applied The approach for environmental and health risk assessments builds on previous work performed for hazardous substances in soil (e.g. VKM 2019, VKM 2014, VKM, 2009, Six and Smolders, 2014). Concentrations of PTEs in soil over time were calculated using a mass balance model, which considers the input by atmospheric deposition, use of fertilisers and soil improvers, as well as loss by leaching, run-off and plant uptake. The resulting first-order differential equation was solved analytically and implemented into Excel®. Run-off and loss by leaching were estimated from data on precipitation, infiltrating fraction and run-off fraction of the water under consideration of the distribution coefficient Kd for the concentration ratio of bulk soil-to-water. This Kd value takes aging sufficiently into account and is thus more realistic than those derived from batch tests. The Kd was estimated separately for each region using established regression equations, with soil pH, organic matter content and clay content as predictors. ...........