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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2020

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Sammendrag

I prosjekt OPTIROT samarbeider 26 produsenter, to kjøleteknologiselskaper, en sensorutvikler, en biopesticidutvikler, to emballasjeprodusenter, fire regioner under NLR, Gartnerhallen og fem forskningsinstitutter (Nofima, Sintef, NMBU, Århus universitet og NIBIO) i gjennomføring av undersøkelser for å redusere svinn under lagring av gulrot, kålrot og sellerirot. Sammendrag av arbeidspakkene: i) Optimal gjødsling med bor og kalsium for bedre lagringskvalitet. Det er testet ut 6 ulike metoder /tidspunkter for tildeling av bor. Vi så liten effekt av de ulike metodene og tidspunktene for tildeling av bor til knollselleri (felter LI & ST) og kålrot (felter APP & INN). Symptomene bormangel og brune flekker ses inne i røtten og bedømmes ved deling av roten. I begge kulturer var det stor variasjon mellom felt. For knollselleri var det størst forekomst av brune flekker og lavest andel friske røtter i ST, som hadde lavest Ca/B-forhold i blader, lavest innhold av Zn i blader og røtter og lavest pH. I kålrot var det symptomer på bormangel i begge felt i 2017, sterkest i INN. For begge felt var symptomene sterkest i behandlingr uten tilførsel av B. Andel friske røtter var lavest i INN. Vi anbefaler flere undersøkelser for å konkludere ytterligere....

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Sammendrag

The stramenopile alga Nannochloropsis evolved by secondary endosymbiosis of a red alga by a heterotrophic host cell and emerged as a promising organism for biotechnological applications, such as the production of polyunsaturated fatty acids and biodiesel. Peroxisomes play major roles in fatty acid metabolism but experimental analyses of peroxisome biogenesis and metabolism in Nannochloropsis are not reported yet. In fungi, animals, and land plants, soluble proteins of peroxisomes are targeted to the matrix by one of two peroxisome targeting signals (type 1, PTS1, or type 2, PTS2), which are generally conserved across kingdoms and allow the prediction of peroxisomal matrix proteins from nuclear genome sequences. Because diatoms lost the PTS2 pathway secondarily, we investigated its presence in the stramenopile sister group of diatoms, the Eustigmatophyceae, represented by Nannochloropsis. We detected a full-length gene of a putative PEX7 ortholog coding for the cytosolic receptor of PTS2 proteins and demonstrated its expression in Nannochloropsis gaditana. The search for predicted PTS2 cargo proteins in N. gaditana yielded several candidates. In vivo subcellular targeting analyses of representative fusion proteins in different plant expression systems demonstrated that two predicted PTS2 domains were indeed functional and sufficient to direct a reporter protein to peroxisomes. Peroxisome targeting of the predicted PTS2 cargo proteins was further confirmed in Nannochloropsis oceanica by confocal and transmission electron microscopy. Taken together, the results demonstrate for the first time that one group of stramenopile algae maintained the import pathway for PTS2 cargo proteins. To comprehensively map and model the metabolic capabilities of Nannochloropsis peroxisomes, in silico predictions needs to encompass both the PTS1 and the PTS2 matrix proteome.

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Sammendrag

The effect of tree growth-control technologies on apple tree vegetative development, productivity and fruit quality was investigated with apple cultivar ‘Rubin’ on dwarf rootstock P 60 at the Institute of Horticulture, Lithuanian Re-search Centre for Agriculture and Forestry in 2015–2018. Eight treatments were established combining tree trunk incision by chainsaw before flowering, application of prohexadione-calcium in different dose and time, summer pruning in August and root pruning before flowering. Root pruning from both sides of the tree significantly reduced tree trunk diameter, shoot length and pruning weights but at the same time reduced fruit weight. It increased tree productivity and enhanced fruit colouring. Two applications of prohexadione-calcium significantly reduced mean shoot length and increased average fruit weight. Summer pruning had a positive impact on fruit colouring. Trunk incisions enhanced leaf P, K and Fe content. Pro-Ca increased leaf Ca content. Trees root pruned from both sides had one of the lowest contents of all tested minerals. All tree growth-control technologies had a positive impact on tree productivity , fruit quality and bearing stability comparing with control treatment.