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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2020

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Late-spring frosts (LSFs) affect the performance of plants and animals across the world’s temperate and boreal zones, but despite their ecological and economic impact on agriculture and forestry, the geographic distribution and evolutionary impact of these frost events are poorly understood. Here, we analyze LSFs between 1959 and 2017 and the resistance strategies of Northern Hemisphere woody species to infer trees’ adaptations for minimizing frost damage to their leaves and to forecast forest vulnerability under the ongoing changes in frost frequencies. Trait values on leaf-out and leaf-freezing resistance come from up to 1,500 temperate and boreal woody species cultivated in common gardens. We find that areas in which LSFs are common, such as eastern North America, harbor tree species with cautious (late-leafing) leaf-out strategies. Areas in which LSFs used to be unlikely, such as broad-leaved forests and shrublands in Europe and Asia, instead harbor opportunistic tree species (quickly reacting to warming air temperatures). LSFs in the latter regions are currently increasing, and given species’ innate resistance strategies, we estimate that ∼35% of the European and ∼26% of the Asian temperate forest area, but only ∼10% of the North American, will experience increasing late-frost damage in the future. Our findings reveal region-specific changes in the spring-frost risk that can inform decision-making in land management, forestry, agriculture, and insurance policy.

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Biological control of pests is a growing market in the world. It is expected that the use entomopathogenic fungi to control pests will take an important share of this market. Most fungal products in the world are based on aerial conidia produced by solid fermentation using cereal grains. An alternative for aerial conidia is the use of blastospores, yeast-like hydrophilic cells that can be produced in large amounts by liquid fermentation in a short time (<4 days), in a small space and with low hand labor compared to the solid fermentation method. Therefore, the main objectives of the present studies were first to optimize a liquid culture medium for low cost production of Metarhizium blastopores; second: to assess the bioactivity of air-dried blastospores against the cattle-tick Rhipicephalus microplus and the corn-leafhopper Dalbulus maidis; third: to develop an air-dried and spray-dried Metarhizium blastospore formulation with bioactivity against the corn-leafhopper D. maidis; fourth: to improve the shelf-life of the best air-dried and spray-dried formulations stored in refrigerated (± 4°C) and in ambient conditions (28°C) using oxygen and moistures absorbrs or vacuum and fifth: to use comparative genome-wide transcriptome analyses to determine changes in gene expression between the filamentous and blastospore growth phases in vitro to characterize physiological changes and metabolic signatures associated with M. anisopliae and M. rileyi dimorphism. We showed that blastospore production of Metarhizium is isolate- and species-dependent.Glucose-enriched cultures inoculated with pre-cultures improved yields reaching optimal growth for Metarhizium robertsii ESALQ1426 (5.9 × 108 blastospores/mL) within 2 d. We argue that both osmotic pressure, induced by high glucose titers, and isolate selection are critical to produce high yields of blastospores. [...]