Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2010
Forfattere
Venche Talgø Gary A. Chastagner Iben M. Thomsen Thomas Cech Kathy Rily Kurt Lange Sonja Klemsdal Arne StensvandSammendrag
For several decades, current-season needle necrosis (CSNN) has been a serious foliage disorder on noble fir (Abies procera) and Nordmann fir (A. nordmanniana) in European and North American Christmas tree and bough plantations. Randomly distributed needles in the new foliage develop chlorotic spots or bands that later may turn necrotic and cause heavy needle cast. We isolated Sydowia polyspora from symptomatic Nordmann fir needles from Austria, Denmark, Germany, Norway, Slovakia, and the U.S., and from Nordmann fir seeds produced in Austria, Denmark, Georgia, and Russia. We also isolated the fungus from noble, Turkish (A. bornmulleriana), grand (A. grandis), and subalpine (A. lasiocarpa) fir needles, and from noble and subalpine fir seeds. Furthermore, S. polyspora was isolated from needles with a missing wax layer around the stomata. Inoculation tests with S. polyspora produced CSNN symptoms on Nordmann fir seedlings and transplants. CSNN spread from tree to tree in a trial in a plastic tunnel in Norway.
Sammendrag
Sølvglans som er årsaka av soppen Chondrostereum purpureum, er ein relativt vanleg og svært skadeleg sjukdom i fleire treaktige prydplanter og i alle fruktartane våre. I 2007 fann vi omfattande skade av sølvglans på eldre planter av søtmispel (Amelanchier sp.) i ein hekk på Ås.
Sammendrag
Dette er en kort beskrivelse av været i vekstsesongen 2010 basert på data registrert ved Bioforsks klimastasjoner og noen detaljer hentet fra klimatologiske oversikter utgitt av Meteorologisk institutt.
Sammendrag
The gas chromatography mass spectrometry (GC-MS) deconvolution reporting software (DRS) from Agilent Technologies has been evaluated for its ability as a screening tool to detect a large number ofpesticides in incurred and fortified samples extracted with acetone/dichloromethane/light petroleum(Mini-Luke method). The detection of pesticides is based on fixed retention times using retention timelocking (RTL) and full scan mass spectral comparison with a partly customer built automated massspectral deconvolution and identification system (AMDIS) database. The GC-MS was equipped with a programmable temperature vaporising (PTV) injector system which enables more sample to be injected.In a blind study of 52 real samples a total number of 158 incurred pesticides were found. In addition to the 85 pesticides found by manual interpretation of GC-NPD/ECD chromatograms, the DRS revealed 73 morepesticides (+46%). The DRS system also shows its potential to discover pesticides which are normally notsearched for (EPN in long beans from Thailand). A spiking experiment was performed to blank matricesof apple, orange and lettuce with 177 different pesticides at concentration levels 0.02 and 0.1 mg/kg. The samples were analysed on GC-MS full scan and the AMDIS match factor was used as a mass spectralquality criterion. The threshold level of the AMDIS match factor was set at 20 to eliminate most of thefalse positives. AMDIS match factors from 20 up to 69 are regarded only as indication of a positive hit andmust be followed by manual interpretation. Pesticides giving AMDIS match factors at ≥70 are regarded as identified. To simplify and decrease the large amount of data generated at each concentration level,the AMDIS match factors ≥20 was averaged (mean AMF) for each pesticide including the commodities and their replicates. Among 177 different pesticides spiked at 0.02 and 0.1 mg/kg level, the percentage of mean AMF values ≥70 were 23% and 80%, respectively. For 531 individual detections of pesticides (177pesticides×3 replicates) giving AMDIS match factor 20 in apple, orange and lettuce, the detection rates at 0.02 mg/kg were 71%, 63% and 72%, respectively. For the 0.1 mg/kg level the detection rates were 89%,85% and 89%, respectively. In real samples some manual interpretation must be performed in addition. However, screening by GC-MS/DRS is about 5-10 times faster compared to screening with GC-NPD/ECDbecause the time used for manual interpretation is much shorter and there is no need for re-injection on GC-MS for the identification of suspect peaks found on GC-NPD/ECD.
Forfattere
Jens Rohloff Per Winge Jahn Davik May Bente Brurberg K Mockaitis V Shulaev SK Randall Atle Bones Muath AlsheikhSammendrag
In order to support functional genomics research in octoploid (Fragaria x ananassa Duch.) and diploid (F. vesca) strawberry, a customized Fragaria microarray chip was developed as a joint collaboration between Graminor Breeding Ltd. and NTNU. F. vesca cDNA sequences were provided by The Center for Genomics and Bioinformatics, Indiana University (an assembly of >3 million reads from GS-FLX Titanium - Roche/454 Life Sciences sequencing), and about 59,000 publicly available Fragaria EST sequences were uploaded from NCBI. In addition, ~190 Mb of preliminary draft genome sequences from F. vesca were provided by the Strawberry Genome Sequencing Consortium (courtesy to V. Shulaev). cDNAs used as templates for probe design were validated by BlastN against the F. vesca draft genome excluding cDNAs of microbial origin. Genes not represented in the cDNA collection were identified by screening F. vesca draft genome against protein sequences from Arabidopsis thaliana, Vitis vinifera, Ricinus communis and Populus trichocarpa. Exon sequences from genes not found in the cDNAs were included. In total, 43723 unique 60-mer probes were designed and the Agilent eARRAY tool was used to produce a 4x44k format microarray chip. Fragaria chip applicability and feasibility for transcriptional profiling was investigated using either abiotic (low temperature) or biotic (pathogenic fungi) stress treatment. Microarray data will be subsequently integrated with other omics data to address gene-regulatory networks and biological functions. Cold acclimation experiments were focused on short- and long-term effects in meristematic tissue, and revealed the up-regulation of ~100 cold-responsive genes (transcription factors, dehydrins, enzymes), and transcripts involved in starch breakdown and raffinose biosynthesis. Beside central metabolism, secondary metabolism was also strongly modulated as seen by changes in the expression of flavonoid biosynthesis-related genes. Time-course studies of transcriptional responses in F. vesca accessions showing contrasting resistance toward the pathogen Phytophthora cactorum are in progress, and will be presented in-depth.
Forfattere
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Anne Marte Tronsmo Bjørn Økland Leif SundheimSammendrag
Det er ikke registrert sammendrag
Forfattere
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimSammendrag
Det er ikke registrert sammendrag
Forfattere
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Roseroot, Rhodiola rosea, is a perennial herbaceous plant of the family Crassulaceae. The rhizomes of 95 roseroot clones in the Norwegian germplasm collection were analysed and quantified for their content of the bioactive compounds rosavin, salidroside, rosin, cinnamyl alcohol and tyrosol using HPLC analysis. All five bioactive compounds were detected in all 95 roseroot clones but in highly variable quantities. The ranges observed for the different compounds were for rosavin 2.90-85.95mgg-1, salidroside 0.03-12.85mgg-1, rosin 0.08-4.75mgg-1, tyrosol 0.04-2.15mgg-1 and cinnamyl alcohol 0.02-1.18mgg-1. The frequency distribution of the chemical content of each clone did not reflect a certain geographic region of origin or the gender of the plant. Significant correlations were found for the contents of several of these bioactive compounds in individual roseroot clones. A low, but not significant correlation was found between AFLP markers previously used to study the genetic diversity of the roseroot clones and their production of the chemical compounds. The maximum level of rosavin, rosin and salidroside observed were higher than for any roseroot plant previously reported in literature, and the roseroot clones characterized in this study might therefore prove to be of high pharmacological value.