Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Heli Fitzgerald Jens Weibull Kristina Bjureke Dag Terje Filip Endresen Jenny Hagenblad Marko Hyvärinen Elina Kiviharju Morten Rasmussen Hjörtur Þorbjörnsson Anna PalméSammendrag
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Forfattere
Heli Fitzgerald Jens Weibull Kristina Bjureke Dag Terje Filip Endresen Jenny Hagenblad Marko Hyvärinen Elina Kiviharju Morten Rasmussen Hjörtur Þorbjörnsson Anna PalméSammendrag
Vilda kulturväxtsläktingar är helt nödvändiga för att vi ska kunna hantera framtida utmaningar som rör tryggad livsmedelstillgång, ett miljömässigt hållbart jordbruk och att anpassa våra grödor till klimatförändringar.
Forfattere
Merike Sõmera Anders Kvarnheden Cécile Desbiez Dag-Ragnar Blystad Pille Sooväli Jiban Kumar Kundu Mark Gantsovski Jim Nygren Hervé Lecoq Eric Verdin Carl Jonas Jorge Spetz Lucie Tamisier Erkki Truve Sebastien MassartSammendrag
High-throughput sequencing technologies were used to identify plant viruses in cereal samples surveyed from 2012 to 2017. Fifteen genome sequences of a tenuivirus infecting wheat, oats, and spelt in Estonia, Norway, and Sweden were identified and characterized by their distances to other tenuivirus sequences. Like most tenuiviruses, the genome of this tenuivirus contains four genomic segments. The isolates found from different countries shared at least 92% nucleotide sequence identity at the genome level. The planthopper Javesella pellucida was identified as a vector of the virus. Laboratory transmission tests using this vector indicated that wheat, oats, barley, rye, and triticale, but none of the tested pasture grass species (Alopecurus pratensis, Dactylis glomerata, Festuca rubra, Lolium multiflorum, Phleum pratense, and Poa pratensis), are susceptible. Taking into account the vector and host range data, the tenuivirus we have found most probably represents European wheat striate mosaic virus first identified about 60 years ago. Interestingly, whereas we were not able to infect any of the tested cereal species mechanically, Nicotiana benthamiana was infected via mechanical inoculation in laboratory conditions, displaying symptoms of yellow spots and vein clearing evolving into necrosis, eventually leading to plant death. Surprisingly, one of the virus genome segments (RNA2) encoding both a putative host systemic movement enhancer protein and a putative vector transmission factor was not detected in N. benthamiana after several passages even though systemic infection was observed, raising fundamental questions about the role of this segment in the systemic spread in several hosts.
Forfattere
Fernanda Canassa Celeste P. D'Alessandro Sidcleide B. Sousa Clarice G. B. Demétrio Nicolai Vitt Meyling Ingeborg Klingen Italo Delalibera Jr.Sammendrag
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Forfattere
Amar Telke Kirill Ovchinnikov Kiira Vuoristo Geir Mathiesen Tage Thorstensen Dzung B. DiepSammendrag
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Forfattere
Wiktoria Kaczmarek-Derda Marit Helgheim Jan Netland Hugh Riley Kjell Wærnhus Samson Øpstad Liv Østrem Lars Olav BrandsæterSammendrag
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Forfattere
Min-Rui Wang Zhibo Hamborg Jiří Zámečník Alois Bilavčík Dag-Ragnar Blystad Sissel Haugslien Qiao-Chun WangSammendrag
The present study described a droplet-vitrification cryopreservation for shoot tips of shallot (Allium cepa var. aggregatum), a small bulb onion. Shoot tips taken from in vitro stock shoots were precultured with 0.3 M and 0.5 M of sucrose, with 1 day for each concentration. Precultured shoot tips were treated with a loading solution containing 2 M glycerol and 0.6 M sucrose for 20 min and then exposed to plant vitrification solution 3 (PVS3) at 24 °C for 3 h of dehydration. Following exposure to PVS3, shoot tips were moved onto 5.0 μl PVS3 droplets on aluminum foil strips, followed by direct immersion into liquid nitrogen for 1 h. Frozen shoot tips were thawed by incubation in liquid MS medium containing 1.2 m sucrose for 20 min at room temperature, and then post-thaw cultured for shoot regrowth. Exposure of the shoot tips to PVS3 produced shoot regrowth (58%). Differential scanning calorimetry (DSC) detected 1.8% of freezable water in the shoot tips that had been dehydrated by PVS2, and no freezable water in those by PVS3 treatment. Exposure to PVS3 provided a broader safe temperature range (− 196 °C to − 88 °C), compared to that (− 196 °C to − 116 °C) of PVS2, for cryopreserved samples. Histological observations found that PVS3 dehydration allowed many cells in the apical dome and in the leaf primordia to survive following freezing in LN, while PVS2 dehydration resulted in much fewer surviving cells in the apical dome. The droplet-vitrification cryopreservation produced 56%, 72% and 32% shoot regrowth in cryopreserved shoot tips taken from in vitro shoots, adventitious buds regenerated from stem discs and field-grown bulbs, respectively. Advantages and disadvantages of the use of different source explants for cryopreservation were discussed. The droplet-vitrification cryopreservation produced 45% and 70% shoot regrowth in the additional two shallot genotypes ‘Kverve’ and ‘Lunteviga’. The results obtained in this study provide technical supports for setting-up cryo-bankings of genetic resources of shallots and other Allium species.
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