Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2020
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Tomasz Leszek Woznicki Krzysztof Kusnierek Sofie Andersen Kristian Rindal Unni Myrheim Roos Anita SønstebySammendrag
Det er ikke registrert sammendrag
Forfattere
Mette Thomsen Erlend Indergård Pia Heltoft Thomsen Gerd Guren Anne-Berit Wold Hanne Larsen Belachew Asalf TadesseSammendrag
I prosjekt OPTIROT samarbeider 26 produsenter, to kjøleteknologiselskaper, en sensorutvikler, en biopesticidutvikler, to emballasjeprodusenter, fire regioner under NLR, Gartnerhallen og fem forskningsinstitutter (Nofima, Sintef, NMBU, Århus universitet og NIBIO) i gjennomføring av undersøkelser for å redusere svinn under lagring av gulrot, kålrot og sellerirot. Sammendrag av arbeidspakkene: i) Optimal gjødsling med bor og kalsium for bedre lagringskvalitet. Det er testet ut 6 ulike metoder /tidspunkter for tildeling av bor. Vi så liten effekt av de ulike metodene og tidspunktene for tildeling av bor til knollselleri (felter LI & ST) og kålrot (felter APP & INN). Symptomene bormangel og brune flekker ses inne i røtten og bedømmes ved deling av roten. I begge kulturer var det stor variasjon mellom felt. For knollselleri var det størst forekomst av brune flekker og lavest andel friske røtter i ST, som hadde lavest Ca/B-forhold i blader, lavest innhold av Zn i blader og røtter og lavest pH. I kålrot var det symptomer på bormangel i begge felt i 2017, sterkest i INN. For begge felt var symptomene sterkest i behandlingr uten tilførsel av B. Andel friske røtter var lavest i INN. Vi anbefaler flere undersøkelser for å konkludere ytterligere....
Sammendrag
The stramenopile alga Nannochloropsis evolved by secondary endosymbiosis of a red alga by a heterotrophic host cell and emerged as a promising organism for biotechnological applications, such as the production of polyunsaturated fatty acids and biodiesel. Peroxisomes play major roles in fatty acid metabolism but experimental analyses of peroxisome biogenesis and metabolism in Nannochloropsis are not reported yet. In fungi, animals, and land plants, soluble proteins of peroxisomes are targeted to the matrix by one of two peroxisome targeting signals (type 1, PTS1, or type 2, PTS2), which are generally conserved across kingdoms and allow the prediction of peroxisomal matrix proteins from nuclear genome sequences. Because diatoms lost the PTS2 pathway secondarily, we investigated its presence in the stramenopile sister group of diatoms, the Eustigmatophyceae, represented by Nannochloropsis. We detected a full-length gene of a putative PEX7 ortholog coding for the cytosolic receptor of PTS2 proteins and demonstrated its expression in Nannochloropsis gaditana. The search for predicted PTS2 cargo proteins in N. gaditana yielded several candidates. In vivo subcellular targeting analyses of representative fusion proteins in different plant expression systems demonstrated that two predicted PTS2 domains were indeed functional and sufficient to direct a reporter protein to peroxisomes. Peroxisome targeting of the predicted PTS2 cargo proteins was further confirmed in Nannochloropsis oceanica by confocal and transmission electron microscopy. Taken together, the results demonstrate for the first time that one group of stramenopile algae maintained the import pathway for PTS2 cargo proteins. To comprehensively map and model the metabolic capabilities of Nannochloropsis peroxisomes, in silico predictions needs to encompass both the PTS1 and the PTS2 matrix proteome.
Forfattere
Nobertas Uselis Jonas Viškelis Juozas Lanauskas Mindaugas Liaudanskas Valdimaras Janulis Darius KviklysSammendrag
The effect of tree growth-control technologies on apple tree vegetative development, productivity and fruit quality was investigated with apple cultivar ‘Rubin’ on dwarf rootstock P 60 at the Institute of Horticulture, Lithuanian Re-search Centre for Agriculture and Forestry in 2015–2018. Eight treatments were established combining tree trunk incision by chainsaw before flowering, application of prohexadione-calcium in different dose and time, summer pruning in August and root pruning before flowering. Root pruning from both sides of the tree significantly reduced tree trunk diameter, shoot length and pruning weights but at the same time reduced fruit weight. It increased tree productivity and enhanced fruit colouring. Two applications of prohexadione-calcium significantly reduced mean shoot length and increased average fruit weight. Summer pruning had a positive impact on fruit colouring. Trunk incisions enhanced leaf P, K and Fe content. Pro-Ca increased leaf Ca content. Trees root pruned from both sides had one of the lowest contents of all tested minerals. All tree growth-control technologies had a positive impact on tree productivity , fruit quality and bearing stability comparing with control treatment.
Sammendrag
One of the most important factors affecting photosynthesis and metabolism is light absorbance by leaves and penetration through the canopy. The aim of this study was to evaluate the influence of planting density and tree development stages on photosynthetic activity, photosynthetic pigments, and carbohydrates in apple (Malus domestica Borkh.) trees in a combined way. The apple tree, Auksis, was grafted on dwarfing rootstock P 22. Space between rows was 3 m, trees were planted in 2001 in four distances: 0.25 m, 0.50 m, 0.75 m, and 1.00 m. Measurements and leaf samples were taken in the end of May (leaves fully expanded BBCH 20–25), in the middle of July (beginning of apple maturity BBCH 73–75) and at the end of August (harvest time BBCH 87–88) according BBCH—growth stages. Photosynthetic rate was significantly the lowest in the spring and tended to rise until fruit ripening, when it increased up to 19.4% compared to spring. Significantly the highest chlorophyll b and carotene α and β contents were found at the BBCH 73–75. The lowest levels of fructose and sorbitol in leaves were found at BBCH 73–75. The amount of starch accumulated in the leaves increased three times in summer compared to spring. Reduced distance between trees to four times (from 1 m to 0.25 m) showed clear competitive stress, as the decrease of photosynthetic rate (up to 36.4–38.6%) and total starch (up to 37–53%) was observed. The photosynthetic behaviour of apple trees was significantly affected by the development stage during the particular season which is related with physiological changes of metabolites transport and their distribution during fruit ripening and leaf senescence.
Forfattere
Nobertas Uselis Jonas Viškelis Juozas Lanauskas Mindaugas Liaudanskas Valdimaras Janulis Darius KviklysSammendrag
A study on the influence of planting distances on the growth, productivity and fruit quality of dwarf apple trees in a 15- to 18-year-old orchard was carried out at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry. Trees of the apple (Malus × domestica Borkh) cultivar ‘Auksis’ on rootstock P 60 were planted at distances of 3 × 1.5, 3 × 1.25, 3 × 1.00 m and 3 × 0.75 m. With increasing density of fruit trees, single-tree growth, generative development and yield were significantly reduced. The opposite results were obtained when these parameters were evaluated per unit area. Yield and fruit quality measurements were made at two canopy heights: 0–1.5 and 1.5–2.5 m. In the upper part of the fruit tree canopy, fruit average weight and diameter were higher, while the colour was more intense and less dependent on the planting density of fruit trees. In the lower part of the canopy, fruit quality was inferior and with increasing fruit tree density it further deteriorated. Planting distances had a significant effect on the accumulation of sugar, soluble solids and dry matter content: greater planting distances resulted in increased sugar content from 10.97% to 11.90%, soluble solids – from 12.30% to 13.17% and dry matter content – from 13.80% to 14.80%. Conversely, higher accumulation of phenolic and triterpenic compounds in apple fruits was found with decreasing planting distances. A significant increase of phenolic compounds from 2.91 up to 4.03 mg g-1 DW (dry weight) was recorded at the upper part of the canopy, while an increase of triterpens from 12.9 up to 16.07 mg g-1 DW – at the lower part of the canopy. The best productivity and fruit quality of ‘Auksis’ apple trees on P 60 rootstock at the full bearing stage were obtained, when fruit trees had been spaced at 3 × 1.25 m. Key words: fruit biochemical composition, fruit colour, Malus × domestica, planting system.
Sammendrag
Adjustable crop load primarily involves bud manipulation, and usually switches from vegetative to reproductive buds. While this switch is not fully understood, it is still controlled by the ratio of hormones, which promote or inhibit bud formation. To determine the reasons for biennial bearing, the effect of apple rootstock, scion cultivar, crop load, as well as metabolic changes of endogenous phytohormones [zeatin, jasmonic acid, indole-3 acetic acid (IAA), abscisic acid (ABA), and gibberellins 1, 3, and 7 (GAs)], and soluble sugars (glucose, fructose, and sorbitol) were evaluated, and their connections with return bloom and yield of apple tree buds were analyzed. Cultivars “Ligol” and “Auksis” were tested on five rootstocks contrasting in induced vigor: semi-dwarfing M.26; dwarfing M.9, B.396, and P 67; and super-dwarfing P 22. Crop load levels were adjusted before flowering, leaving 75, 113, and 150 fruits per tree. Principal component analysis (PCA) scatter plot of the metabolic response of phytohormones and sugars indicated that the effect of the semi-dwarfing M.26 rootstock was significantly different from that of the dwarfing M.9 and P 67, as well as the super-dwarfing P 22 rootstocks in both varieties. The most intensive crop load (150 fruits per tree) produced a significantly different response compared to less intensive crop loads (113 and 75) in both varieties. In contrast to soluble sugar accumulation, increased crop load resulted in an increased accumulation of phytohormones, except for ABA. Dwarfing rootstocks M.9, B.396, and P 67, as well as super-dwarf P 22 produced an altered accumulation of promoter phytohormones, while the more vigorous semi-dwarfing M.26 rootstock induced a higher content of glucose and inhibitory phytohormones, by increasing content of IAA, ABA, and GAs. The most significant decrease in return bloom resulted from the highest crop load in “Auksis” grafted on M.9 and P 22 rootstocks. Average difference in flower number between crop loads of 75 and 150 fruits per tree in “Ligol” was 68%, while this difference reached ~ 90% for P 22, and ~ 75% for M.9 and M.26 rootstocks. Return bloom was dependent on the previous year’s crop load, cultivar, and rootstock.
Forfattere
Aurita Butkeviciute Mindaugas Liaudanskas Darius Kviklys Dalia Gelvonauskiene Valdimaras JanulisSammendrag
As the interest in heirloom cultivars of apple trees, their fruit, and processed products is growing worldwide, studies of the qualitative and quantitative composition of biological compounds are important for the evaluation of the quality and nutritional properties of the apples. Studies on the variations in the chemical composition of phenolic compounds characterized by a versatile biological effect are important when researching the genetic heritage of the heirloom cultivars in order to increase the cultivation of such cultivars in orchards. A variation in the qualitative and quantitative composition of phenolic compounds was found in apple samples of cultivars included in the Lithuanian collection of genetic resources. By the high-performance liquid chromatography (HPLC) method flavan-3-ols (procyanidin B1, procyanidin B2, procyanidin C2, (+)-catechin and (−)-epicatechin), flavonols (rutin, hyperoside, quercitrin, isoquercitrin, reynoutrin and avicularin), chlorogenic acids and phloridzin were identified and quantified in fruit samples of heirloom apple cultivars grown in Lithuania. The highest sum of the identified phenolic compounds (3.82 ± 0.53 mg/g) was found in apple fruit samples of the ‘Koštelė’ cultivar
Forfattere
Mindaugas Liaudanskas Rugile Okuleviciute Juozas Lanauskas Darius Kviklys Kristina Zymone Tamara Rendyuk Vaidotas Žvikas Nobertas Uselis Valdimaras JanulisSammendrag
The aim of this study was to determine the composition and content of phenolic compounds in extracts of plum fruit. Fruit of 17 plum cultivars were analyzed. Fruit samples were collected in 2019 from fruit trees with “Myrobalan” (P. cerasifera Ehrh.) and “Wangenheim Prune” (P. domestica L.) rootstocks. The following glycosides of the flavonol group were identified: avicularin, isorhamnetin-3-O-rutinoside, isoquercitrin, hyperoside, rutin, and an aglycone quercetin. Compounds of the flavan-3-ol group were identified, such as (+)-catechin, procyanidin C1, and procyanidin A2, along with chlorogenic acid attributed to phenolic acids and a non-phenolic cyclitol–quinic acid. Of all the analytes identified in plum fruit samples, quinic acid predominated, while chlorogenic acid predominated among all the identified phenolic compounds, and rutin predominated in the flavonol group. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) revealed that fruit samples of “Kubanskaya Kometa”, “Zarechnaya Raniaya”, “Duke of Edinburgh”, “Jubileum”, and “Favorita del Sultano” cultivars had different quantitative content of phenolic compounds from that observed in other samples. The highest total amount of phenolic compounds was found in the European plum samples of the “Zarechnaya Rannyaya” cultivar, while the amount of quinic acid was the highest in plum fruit samples of the “Jubileum” cultivar.