Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2023
Forfattere
Mathias Amundsen Kjersti Aaby Laura Jaakola Gesine Schmidt Inger Martinussen Anne Linn HykkerudSammendrag
Lingonberries (Vaccinium vitis-idaea L.) have received much positive attention due to their exotic taste and high phenolic content. These small red fruits grow across Norway, a country with large variations in abiotic and biotic growth conditions. The large variations in abiotic and biotic growth conditions have potential to influence quality and availability of lingonberries. A three-year study (2019-2021) with 64 field plots across Norway have therefore been set up, with the aim of studying the effect of climate and growth conditions on lingonberries. Here, anthocyanin content in berries from the first growth season is presented. Eight locations across Norway (58 to 69°N) with supposed high production potential of lingonberries were selected. Within each location, eight stands (250 m2) with different biotic conditions were chosen. Berries from each sector were lyophilised and extracted with 70% methanol. Phenolic compounds were analysed by HPLC-DAD-MSn, with quantification of anthocyanin at 520 nm and MS used for identification. The three major anthocyanins in Norwegian lingonberries were cyanidin-3-galactoside (69-90%), -arabinoside (6-23%) and -glucoside (2-10%). Additionally, small quantities of three other cyanidin glycosides were preliminarily identified. The total content of anthocyanins in lingonberries ranged from approximately 320 to 790 mg 100 g‑1 dw. There appears to be a variation in anthocyanin concentration linked to latitude. However, as the variation was as large within the stands of each location as they were between the locations, different growth factors would also play key parts in synthesis of anthocyanins in lingonberries. Results from analysis of berries collected in 2020 and 2021 are necessary to have the basis to draw a conclusion on how biotic and abiotic factors influence anthocyanin content of lingonberries.
Forfattere
Joel Abbey Sherin Jose David Percival Laura Jaakola Samuel K. AsieduSammendrag
Botrytis blight is an important disease of wild blueberry [(Vaccinium angustifolium (Va) and V. myrtilloides (Vm))] with variable symptoms in the field due to differences in susceptibility among blueberry phenotypes. Representative blueberry plants of varying phenotypes were inoculated with spores of B. cinerea. The relative expression of pathogenesis-related genes (PR3, PR4), flavonoid biosynthesis genes, and estimation of the concentration of ten phenolic compounds between uninoculated and inoculated samples at different time points were analyzed. Representative plants of six phenotypes (brown stem Va, green stem Va, Va f. nigrum, tall, medium, and short stems of Vm) were collected and studied using qRT-PCR. The expression of targeted genes indicated a response of inoculated plants to B. cinerea at either 12, 24, 48 or 96 h post inoculation (hpi). The maximum expression of PR3 occurred at 24 hpi in all the phenotypes except Va f. nigrum and tall stem Vm. Maximum expression of both PR genes occurred at 12 hpi in Va f. nigrum. Chalcone synthase, flavonol synthase and anthocyanin synthase were suppressed at 12 hpi followed by an upregulation at 24 hpi. The expression of flavonoid pathway genes was phenotype-specific with their regulation patterns showing temporal differences among the phenotypes. Phenolic compound accumulation was temporally regulated at different post-inoculation time points. M-coumaric acid and kaempferol-3-glucoside are the compounds that were increased with B. cinerea inoculation. Results from this study suggest that the expression of PR and flavonoid genes, and the accumulation of phenolic compounds associated with B. cinerea infection could be phenotype specific. This study may provide a starting point for understanding and determining the mechanisms governing the wild blueberry-B. cinerea pathosystem.
Forfattere
Kiran Kumar Mohapatra A.K. Nayak R.K. Patra Rahul Tripathi Chinmaya Kumar Swain K.C. Moharana Anjani Kumar Mohammad Shahid Sangita Mohanty Saheed Garnaik Hari Sankar Nayak Simran Mohapatra Sekhar Udaya Nagothu Mehreteab TesfaiSammendrag
Introduction: Conventional rice production techniques are less economical and more vulnerable to sustainable utilization of farm resources as well as significantly contributed GHGs to atmosphere. Methods: In order to assess the best rice production system for coastal areas, six rice production techniques were evaluated, including SRI-AWD (system of rice intensification with alternate wetting and drying (AWD)), DSR-CF (direct seeded rice with continuous flooding (CF)), DSR-AWD (direct seeded rice with AWD), TPR-CF (transplanted rice with CF), TPR-AWD (transplanted rice with AWD), and FPR-CF (farmer practice with CF). The performance of these technologies was assessed using indicators such as rice productivity, energy balance, GWP (global warming potential), soil health indicators, and profitability. Finally, using these indicators, a climate smartness index (CSI) was calculated. Results and discussion: Rice grown with SRI-AWD method had 54.8 % higher CSI over FPR-CF, and also give 24.5 to 28.3% higher CSI for DSR and TPR as well. There evaluations based on the climate smartness index can provide cleaner and more sustainable rice production and can be used as guiding principle for policy makers.
Forfattere
Natthidech Beesa Buncha Chinnasri Anongnuch Sasnarukkit Tida Dethoup Kansiree Jindapunnapat Agnes W. Kiriga Kanan Saikai Solveig Haukeland Danny CoyneSammendrag
The legume cavalcade, Centrosema pascuorum, is used extensively as a cover crop and as a component of conservation agriculture systems. It is also an attractive rotation or cover crop for the management of root-knot nematodes (RKN; Meloidogyne spp.) as it is a non-host. RKN are persistent pests that are well known to be difficult to control. However, the mechanisms governing the non-host status of cavalcade is unknown. The current study established that cavalcade leaves are toxic to RKN as either aqueous extracts or soil amendments. Bioassays conducted using Meloidogyne javanica showed that a 90% concentration of aqueous extract derived from 1-month-old cavalcade leaves (89 mg crude extract ml−1) suppressed nematode hatch (82.9%) and killed infective second-stage juveniles of M. javanica (85.3%). Soil amendments with 1% (w/w) of 1-month-old cavalcade leaves (0.99 mg crude extract g−1 soil) also provided effective control of M. javanica in the glasshouse on okra. One-month-old leaves appeared more effective than 2- or 3-month-old leaves. The soil amendments had no adverse phytotoxic effect on okra seed germination. Our study demonstrates the potential for using cavalcade leaves or extracts to manage RKN. This may be due to the nematicidal activity of the various compounds in the leaves, such as flavonoids, phenols and terpenoids, which should be further assessed.
Forfattere
Benjamin Guillaume Hanane Aroui Boukbida Gerben Bakker Andrzej Bieganowski Yves Brostaux Wim Cornelis Wolfgang Durner Christian Hartmann Bo V. Iversen Mathieu Javaux Joachim Ingwersen Krzysztof Lamorski Axel Lamparter András Makó Ana María Mingot Soriano Ingmar Messing Attila Nemes Alexandre Pomes-Bordedebat Martine van der Ploeg Tobias Karl David Weber Lutz Weihermüller Joost Wellens Aurore DegréSammendrag
The soil water retention curve (SWRC) is a key soil property required for predicting basic hydrological processes. The SWRC is often obtained in the laboratory with non-harmonized methods. Moreover, procedures associated with each method are not standardized. This can induce a lack of reproducibility between laboratories using different methods and procedures or using the same methods with different procedures. The goal of this study was to estimate the inter- and intralaboratory variability of the measurement of the wet part (from 10 to 300 hPa) of the SWRC. An interlaboratory comparison was carried out between 14 laboratories, using artificially constructed, porous reference samples that were transferred between laboratories according to a statistical design. The retention measurements were modelled by a series of linear mixed models using a Bayesian approach. This allowed the detection of sample-to-sample variability, interlaboratory variability, intralaboratory variability and the effects of sample changes between measurements. The greatest portion of the differences in the measurement of SWRCs was due to interlaboratory variability. The intralaboratory variability was highly variable depending on the laboratory. Some laboratories successfully reproduced the same SWRC on the same sample, while others did not. The mean intralaboratory variability over all laboratories was smaller than the mean interlaboratory variability. A possible explanation for these results is that all laboratories used slightly different methods and procedures. We believe that this result may be of great importance regarding the quality of SWRC databases built by pooling SWRCs obtained in different laboratories. The quality of pedotransfer functions or maps that might be derived is probably hampered by this inter- and intralaboratory variability. The way forward is that measurement procedures of the SWRC need to be harmonized and standardized.
Forfattere
Julie Sørlie Paus-Knudsen Henrik Andersen Sveinsson Merete Grung Katrine Borgå Anders NielsenSammendrag
Bumblebees carry out the complex task of foraging to provide for their colonies. They also conduct pollination, an ecosystem service of high importance to both wild plants and entomophilous crops. Insecticides can alter different aspects of bumblebee foraging behavior, including the motivation to leave the hive, finding the right flowers, handling flowers, and the ability to return to the colony. In the present study, we assessed how the neonicotinoid imidacloprid affects bumblebees' foraging behavior after exposure to four different treatment levels, including field-realistic concentrations (0 [control], 1, 10, and 100 μg/L), through sucrose solution over 9 days. We observed the behavior of several free-flying bumblebees simultaneously foraging on artificial flowers in a flight arena to register the bees' complex behavior postexposure. To conduct a detailed assessment of how insecticides affect bumblebee locomotor behavior, we used video cameras and analyzed the recordings using computer vision. We found that imidacloprid impaired learning and locomotor activity level when the bumblebees foraged on artificial flowers. We also found that imidacloprid exposure reduced sucrose solution intake and storage. By using automated analyses of video recordings of bumblebee behavior, we identified sublethal effects of imidacloprid exposure at field-realistic doses. Specifically, we observed negative impacts on consumption of sucrose solution as well as on learning and locomotor activity level. Our results highlight the need for more multimodal approaches when assessing the sublethal effects of insecticides and plant protection products in general.
Forfattere
Samuel W. Wilkinson Adam Hannan Parker Amos Muench Robert Simon Wilson K. Hooshmand M.A. Henderson Emma K. Moffat P.S.C.F. Rocha H. Hipperson J.H.M. Stassen Ana López Sánchez Inge S. Fomsgaard Paal Krokene Melissa Magerøy Jurriaan TonSammendrag
Stress can have long-lasting impacts on plants. Here we report the long-term effects of the stress hormone jasmonic acid (JA) on the defence phenotype, transcriptome and DNA methylome of Arabidopsis. Three weeks after transient JA signalling, 5-week-old plants retained induced resistance (IR) against herbivory but showed increased susceptibility to pathogens. Transcriptome analysis revealed long-term priming and/or upregulation of JA-dependent defence genes but repression of ethylene- and salicylic acid-dependent genes. Long-term JA-IR was associated with shifts in glucosinolate composition and required MYC2/3/4 transcription factors, RNA-directed DNA methylation, the DNA demethylase ROS1 and the small RNA (sRNA)-binding protein AGO1. Although methylome analysis did not reveal consistent changes in DNA methylation near MYC2/3/4-controlled genes, JA-treated plants were specifically enriched with hypomethylated ATREP2 transposable elements (TEs). Epigenomic characterization of mutants and transgenic lines revealed that ATREP2 TEs are regulated by RdDM and ROS1 and produce 21 nt sRNAs that bind to nuclear AGO1. Since ATREP2 TEs are enriched with sequences from IR-related defence genes, our results suggest that AGO1-associated sRNAs from hypomethylated ATREP2 TEs trans-regulate long-lasting memory of JA-dependent immunity.
Forfattere
Ludmila Sromek Eeva Ylinen Mervi Kunnasranta Simo Maduna Tuula Sinisalo Craig T. Michell Kit M. Kovacs Christian Lydersen Evgeny Ieshko Elena Andrievskaya Vyacheslav Alexeev Sonja Leidenberger Snorre Hagen Tommi NymanSammendrag
Studies on host–parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple-enzyme restriction-site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population-genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra-host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.
Sammendrag
Urban green infrastructure is critical for providing a wide range of ecosystem goods and services that benefit the urban population. Past studies have suggested that multifunctionality concerning urban infrastructure services and functions is a prerequisite for targeting effective and impactful urban green infrastructure. Moreover, urban green infrastructure with multiple functions can offer socio-economic and environmental benefits. However, there has been a knowledge gap in the planning literature to elaborate multiple ecosystem functions in urban green infrastructure. In particular, existing methods and approaches are lacking for quantifying and monitoring such ecological services and biodiversity in urban green infrastructures at different spatial scales. Therefore, this research aims to review studies focusing on the multifunctionality concept in urban green infrastructure planning. The study highlights the current status and knowledge gaps through a systematic review. Our analysis revealed that current studies on green infrastructure multifunctionality have focused on five main themes: 1) planning methods for urban green infrastructure, 2) assessment approaches of urban green infrastructure, 3) ecosystem services and their benefits, 4) sustainability and climate adaptation, and 5) urban agriculture. The study found that the five themes are somewhat connected to each other. The study has revealed a knowledge gap regarding incorporating multifunctional green infrastructure in the planning principle. The results suggest at least five critical elements to ensure multiple functions in urban infrastructure. The elements are spatial distribution, optimal distance, integrated network, accessibility, and public participation and engagement. The study further recommends research directions for future analysis on green infrastructure multifunctionality that are critical for urban planning.
Forfattere
Andres Perea Lara Macon Sajidur Rahman Huiying Chen Andrew Cox Shelemia Nyamuryekung'e Huiping Cao Andres F. Cibils Richard Estell Glenn Duff Santiago A. UtsumiSammendrag
Detection of parturition of rangeland cows remotely may be possible using low cost LoRa WAN monitoring systems that are capable of logging and transmitting cow activity and position data in real time. This study evaluated candidate algorithms for early detection of parturition using longitudinal data of cow activity and position collected by GPS and triaxial accelerometers. Trials were conducted at the USDA Jornada Experimental Range from November to December 2022. Five Raramuri Criollo and five Angus x Hereford mature cows were equipped with LoRa WAN tracking collars instrumented with GPS and triaxial accelerometers and monitored through late gestation (> 7 months) while grazing rangeland pastures of 1,230 and 2,200 ha, respectively. Animal location (latitude and longitude) and activity count (Ac) obtained from GPS and accelerometers data, respectively, were collected by receiving stations that transmitted data in real time through a LoRa WAN network. Collars transmitted GPS positions at one-hour intervals and Ac data at two-minute intervals. An operator routinely inspected focal cows in herds to register parturition within approximately 12 h accuracy. Sensor data for 21 days prior to calving were processed to calculate distance traveled (m/h) and activity rate (Ac/h). For each hour interval, the adjusted activity Index IN = activity/distance (Ac/m) was computed to disentangle motion changes not associated with walking activity. Two algorithms were tested. The first considered the temporal deviation (D) of IN for a given hour (X0), compared with the average IN of the same hour in the previous seven days: D = INX0 /(INX-1+ INX-2 + …+ INX-7)/7). The second considered the normalized probability (N) of D for a given hour (X0) compared with the same hour over the previous seven days: N = (INX0-(INX-1+ INX-2 + …+ INX-7)/7)/sd.(INX-0, INX-1, …, INX-7). A threshold for high probability of calving was set when at least three consecutive hours with D >3 or N >0.95 were detected. Both algorithms correctly triggered alerts on actual calving days. Thus, lack of detection or false detections of calving indicated that the sensitivity and specificity for calving detection were both 100%. The normalized method (N) triggered delayed calving alerts in two cases. Furthermore, greater (P < 0.05) number of consecutive hours with D > 3 (5.6 ± 2.1) around actual calving time were detected vs. the number of consecutive hours with N > 0.95 (3.9 ± 1.2), suggesting that the former algorithm was also able to detect longer duration of behaviors associated with calving. Results indicate possibilities for remote detection of the onset and duration of calving behavior (parturition + first nursing hours) of beef cows managed on large rangeland pastures that impose operational challenges for visual inspection of cows during calving. Further tests with a greater number of cows and management systems would be needed to confirm this hypothesis.