Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2007
Sammendrag
We evaluated direct and interactive effects of light quality and intensity, temperature and light, diurnal rhythms, and timing of high relative humidity during long day lengths on sporulation of Bremia lactucae, the causal agent of lettuce downy mildew, using inoculated lettuce seedlings and detached cotyledons. Suppression of sporulation by light was strongly dependent upon temperature and there was little suppression at "10°C. The most suppressive waveband was in the range from 400 to 450 nm, although a lesser effect of wavebands from 450 to 500 and 500 to 550 nm could be detected. At 15°C, near the lower threshold for suppression of sporulation by light, a clear diurnal pattern of sporulation was observed independent of light and darkness. This diurnal rhythm potentially could interact with light and temperature to confound the results of controlled environment studies, and may be the controlling factor in timing of sporulation at low temperatures. Forecasting models that currently use sunrise and sunset to delimit periods conducive to sporulation can be adapted to short nights and extended twilight conditions by incorporating the effects reported herein. Additionally, models of sporulation could be adapted to better reflect a decrease or absence of the suppressive effect of light at
Sammendrag
Neozygites floridana is a fungus in the order Entomophthorales that infects and kills the two-spotted spider mite, Tetranychus urticae. The fungus is therefore of interest for the biological control of T. urticae. To obtain information that might help in the use of this fungus under practical conditions in strawberries and cucumbers we have tried to answer the following questions in a series of studies*): 1) When, and at what infection levels does N. floridana occurre in T. urticae populations in fieldgrown strawberries? 2) How and where does N. floridana survive harsh climatic conditions (i.e winter) in Norway? 3) How and where does N. floridana infected T. urticae move and sporulate on a plant? 4) How do commonly used pesticides in strawberries affect N. floridana and T. urticae? 5) How can N. floridana be inoculated in augmentative microbial control of T. urticae? Results show that N. floridana infected and killed T. urticae in 12 out of 12 Norwegian strawberry fields studied. Infection levels up to 90% were observed, and the highest levels were observed late in the season. The infection levels throughout a season varied considerably. N. floridana was observed to overwinter as both hyphal bodies in hibernating T. urticae females from October to at least February at temperatures as low as -20o C. Cadavers with resting spores were found from October to the end of January. Cadavers then probably disintegrated, and resting spores were left on leaves, soil, etc. In a bioassay where a Norwegian N. floridana isolate was tested for numbers and distance of spores thrown at three different temperatures (13o, 18o, 23o C), preliminary results show that high numbers of spores (ca 1300-1900 per cadaver) were thrown at all three temperatures. Further, spores were thrown about the same distance (up to about 6 mm) at all three temperatures. The effects of pesticides used in strawberries on the N. floridana infection level were studied to evaluate factors that might be important for conservation biological control. The pesticides tested were three fungicides; Euparen (tolylfluanid), Teldor (fenhexamid), Switch (cyprodinil +fludioxonil) and one acaricide/ insecticide: Mesurol (methiocarb). The experiment indicated that all three fungicides affect N. floridana negatively but that Euparen might be the least harmful. Mesurol did not affect N. floridana. Our attempts to inoculate N. floridana artificially in a strawberry field has not yet been successful, but we now work on promising methods for inoculation of N. floridana in T. urticae populations in greenhouse cucumbers. More detailed results from the studies referred to in this abstract will soon be published elsewhere.
Sammendrag
To obtain information that might help in the use of Neozygites floridana (Zygomycetes: Entomopthorales) in biological control of Tetranychus urticae (Acari: Tetranychidae), in strawberries and cucumbers we have tried to answer the following questions in a series of studies*): 1) When, and at what infection levels does N. floridana occur in T. urticae populations in field grown strawberries? 2) How does N. floridana survive harsh climatic conditions (i.e winter) in Norway? 3) Where do N. floridana infected T. urticae move and sporulate on a plant? 4) How do commonly used pesticides in strawberries affect N. floridana and T. urticae? 5) How can N. floridana be inoculated in augmentative microbial control of T. urticae? Results show that N. floridana infected and killed T. urticae in 12 out of 12 Norwegian strawberry fields studied. Infection levels up to 90% were observed, and the highest levels were observed late in the season. The infection levels throughout a season varied considerably. N. floridana was observed to over-winter as hyphal bodies in hibernating T. urticae females throughout the winter. Cadavers with resting spores were found from October to the end of January. Cadavers then probably disintegrated, and resting spores were left on leaves, soil, etc. In a bioassay where a Norwegian N. floridana isolate was tested for numbers and distance of spores thrown at three different temperatures (13o, 18o, 23o C), results show that the highest numbers of spores (1886 and 1733 per cadaver) were thrown at 13o and 18o compared to 23o C (1302 per cadaver). Spores were thrown at the same distance (up to about 6 mm) at all three temperatures when cadavers were placed with dorsal side facing up. Cadavers placed with dorsal side down (hanging) threw equal numbers of spores up (on the underside of the leaf in nature) and down (on the leaf below). The effects of pesticides used in strawberries on the N. floridana infection level were studied to evaluate factors that might be important for conservation biological control. The pesticides tested were three fungicides; Euparen (tolylfluanid), Teldor (fenhexamid), Switch (cyprodinil +fludioxonil) and one acaricide/ insecticide: Mesurol (methiocarb). The experiment indicated that all three fungicides affect N. floridana negatively but that Euparen might be the least harmful. Mesurol did not affect N. floridana. Our attempt to inoculate N. floridana artificially in a strawberry field has not yet been successful, but we now work on promising methods for inoculation of N. floridana in T. urticae populations in greenhouse cucumbers. More detailed results from the studies referred to in this abstract will soon be published elsewhere.
Forfattere
Jon Atle Repstad Trygve S. Aamlid Lars Tørres HavstadSammendrag
Norwegian agriculture is totally dependent on a safe supply of seed of winter-hardy timothy varieties. The annual seed consumption varies depending on the extent of winter damages, particularly in northern Norway, and the average seed yield varies with weather and harvest conditions in the seed-producing districts in the southeastern and central part of the country. To buffer these variations, seed companies always keep stocks corresponding to 50-100 % of the average annual seed consumption. Such large stocks are risky to maintain as seed lots will loose germination over time. Our objective was to elucidate the effect of seed harvest time and seed storage location on the longevity of timothy seed. In 2003, timothy `Grindstad" was combined directly on 2, 5 or 8 August corresponding to a seed moisture content (SMC) of 34, 27 and 20 %, respectively. After harvest the seed was dried to 10-11 % SMC. Germination analyses were accomplished 3, 15, 26 and 38 months after seed harvest; the three latter after splitting each seed lot into four sub-lots that were stored either in a conditioned seed store (4ºC, 30% RH), or in unconditioned warehouses at there climatically different locations. While seed harvest time had no effect on germination three months after harvest, differences became increasingly evident as time went by. After 38 months" storage, seed stored in the conditioned store or in the warehouse at the continental location Tynset germinated, on average for harvest times, 15-16 units better than seed stored in the warehouse at the coastal location Vaksdal; and seed lots harvested at 20 % SMC germinated, on average for storage locations, 24 units better than seed harvested at 37 % SMC. While it has long been documented that direct combining at high SMC may damage seed germination, there has been less awareness that this damage may not manifest itself until after a certain storage period.
Forfattere
W.G Flier L.P.N.M Kroon Arne Hermansen H.M.G van Raaij B. Speiser L. Tamm J.G. Fuchs J. Lambion Jafar Razzaghian D. Andrivon S. Wilcockson C. LeifertSammendrag
Genetic variation and pathogenicity of Phytophthora infestans isolates collected from organic potato crops of the susceptible cv. Bintje and the moderately resistant cv. Santé were assesed in France, Norway, and the United Kingdom in 2001 and in Switzerland in 2001 and 2002. Population structures differed considerably between the four P. infestans populations. Those from France, Switzerland and the UK were mainly clonal populations showing restricted levels of genetic diversity, whilst those from Norway were mixed A1 and A2 mating type populations with high levels of genetic diversity, suggesting periodical sexual reproduction. Isolates collected from cv. Bintje were on average more aggressive than or comparable to isolates from cv. Santé. Race complexity varied considerably between the regional P. infestans populations with isolates from France and Switzerland showing the highest number of virulence factors. In all pathogen samples but the French, isolates collected from cv. Santé were more complex than isolates collected from cv. Bintje. No directional selection towards increased aggressiveness towards the more resistant cultivar Santé was observed. This suggests that there is no shift towards increased level of pathogenicity in P. infestans populations following the large-scale introduction of more resistant potato varieties in organic production systems in Europe.
Sammendrag
In Norway, Nordmann (Abies nordmanniana) and subalpine fir (A. lasiocarpa) are the dominant Christmas tree species and Noble fir (A. procera) the dominant species for bough production. In the spring of 2005 a survey was undertaken to determine the presence of fungi on seeds of these three plant species. Twelve seed samples were tested; five from Nordmann fir, four from subalpine fir and three from Noble fir. The test included seeds produced in Austria (Nordmann fir), Canada (subalpine fir), Georgia (Nordmann fir), Norway (Noble and subalpine fir) and Russia (Nordmann fir). The testing was done in a certified seed laboratory in Norway. One hundred seeds per sample were pre-treated in 1 % NaOCl and plated on potato dextrose agar (PDA), and 100 seeds per sample were pre-treated in H2O2 and plated on water agar (WA). The PDA Petri dishes were incubated for 5 to 7 days at 20 + 2 ºC and the WA Petri dishes for 15 to 19 days at 15 + 2 ºC, both under alternating 12 h NUV-light and 12 h darkness. To date not all of the fungi have been identified, but fungi from several genera have been found: Alternaria, Anthostomella, Aspergillus, Aureobasidium, Botrytis, Caloscypha, Cephalosporium, Chaetomium, Cladosporium, Dictyopolyschema, Epicoccum, Fusarium, Mucor, Penicillium, Phoma, Rhizopus, Trichoderma, and Trichothecium. Caloscypha was only found on one subalpine fir seedlot from Canada and Anthostomella on one subalpine fir seed from Norway. All the other fungi were more commonly found in all the samples. Fusarium species are well known to cause damping off in nurseries, but are not reported to cause problems in production fields. Species of Alternaria, Phoma, Botrytis and Trichothecium can damage seedlings. Anthostomella conorum is reported on cone scales of pine (Pinus spp.), but is not described as pathogenic. Aureobasidium is described as a saprophytic or weakly parasitic cosmopolitan fungal genus. Aureobasidium sp. is commonly found in Norway on buds of subalpine fir which fail to open in spring, and it will therefore be included in a pathogenicity test.
Forfattere
Trond HofsvangSammendrag
Artikkelen inneholder minneord om Gudmund Taksdal og hans innsats innen anvendt entomologi, både innenfor norsk jord- og hagebruk og internasjonalt. En oversikt over Taksdals entomologiske publikasjoner fra 1957 til 2003 er også gitt.
Sammendrag
Oil transportation from the Russian part of the Barents Region along the Norwegian coast had insignificant volumes before 2002. However, in 2002 there was a dramatic increase in oil shipment, when 4 million tons of oil was transported across the northern regions. In 2003, the volume reached 8 million tons. The trend continued in 2004, and about 12 million tons of export oil and oil products were delivered from the Russian part of the Barents Region to the western market along the Norwegian coast. In 2005, the oil shipment volumes dropped to 9.5 million tons, and in 2006 increased to 10.5 million tons. In the present report on oil transportation from the Russian North, we have given special attention to the description of the existing and prospective offshore and onshore oil shipment terminals, and their connection to the oil reserves on one hand and to the export routes on the other. In this report we demonstrate that even without a trunk oil pipeline to the Barents Sea coast, the annual oil exports from the Russian part of the Barents Region may reach a volume of about 50-80 million tons in the next decade. About 50 million tons of crude oil and oil products can be delivered by railway to the Murmansk ports in the Barents Sea, and Kandalaksha and Arkhangelsk in the White Sea. In addition, up to 20 million tons of oil will come from the northern oil fields in the Nenets Autonomous Region, and from Prirazlomnoye oil field in the Pechora Sea. Prirazlomnoye is the first offshore industrial oil field in the Russian part of the Barents Region, the operations there will go on all year round, and most of the year in ice-covered waters. Dolginskoye oil field, which is also in the Pechora Sea and estimated to be three times as big as Prirazlomnoye, can produce the first oil in 2013. There will be stable increase in the amounts of oil shipped from Western Siberia. The terminals in the Kara Sea can load 2-3 million tons of crude oil for transhipment in the Kola Bay of the Barents Sea. In the European part of Russia there are three possibilities for shipping oil for export. The first way is through the Black Sea via the Bosporus to the Mediterranean Sea. Another route is through the Baltic Sea via the Gulf of Finland and Kattegat. The third alternative is to transport oil through the Barents Sea along the coasts of north-western Russia and northern Norway. Out of these three options only the northern one, the Barents Sea route, can provide the possibility of stable shipping large amounts directly to European and other major harbours, avoiding the challenges of transit through the neighbouring countries or heavy traffic in the sea straits. Oil pollution prevention should be the central issue during oil transportation in the Barents Sea. In this report we pay attention to the environmental safety matters in oil transportation and Norwegian-Russian co-operation in the oil pollution prevention. The increasing internationalisation of the transport system in the region appears to affect the present trend toward more advanced and safer terminals and vessels that comply with international safety rules. Early warning and notification of ships passing through the Norwegian waters has been used more frequently and on voluntary basis, but still not as often as desired and can be arranged within a bilateral Russian-Norwegian agreement. The establishment of traffic control centres in Vardø and Murmansk will considerably improve the oil spill prevention and response preparedness.
Forfattere
Christopher Saski Seung-Bum Lee Siri Fjellheim Chittibabu Guda Robert K Jansen Hong Luo Jeffrey Tomkins Odd Arne Rognli Henry Daniell jihong liu clarkeSammendrag
Comparisons of complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera to six published grass chloroplast genomes reveal that gene content and order are similar but two microstructural changes have occurred. First, the expansion of the IR at the SSC/IRa boundary that duplicates a portion of the 5 end of ndhH is restricted to the three genera of the subfamily Pooideae (Agrostis, Hordeum and Triticum). Second, a 6 bp deletion in ndhK is shared by Agrostis, Hordeum, Oryza and Triticum, and this event supports the sister relationship between the subfamilies Erhartoideae and Pooideae. Repeat analysis identiWed 19- 37 direct and inverted repeats 30 bp or longer with a sequence identity of at least 90%. Seventeen of the 26 shared repeats are found in all the grass chloroplast genomes examined and are located in the same genes or intergenic spacer (IGS) regions. Examination of simple sequence repeats (SSRs) identiWed 16"21 potential polymorphic SSRs. Five IGS regions have 100% sequence identity among Zea mays, Saccharum oYcinarum and Sorghum bicolor, whereas no spacer regions were identical among Oryza sativa, Triticum aestivum, H. vulgare and A. stolonifera despite their close phylogenetic relationship. Alignment of EST sequences and DNA coding sequences identified six C"U conversions in both Sorghum bicolor and H. vulgare but only one in A. stolonifera. Phylogenetic trees based on DNA sequences of 61 protein-coding genes of 38 taxa using both maximum parsimony and likelihood methods provide moderate support for a sister relationship between the subfamilies Erhartoideae and Pooideae.
Sammendrag
Foreløpige resultater på effekt av økning i temperatur og/eller CO2, samt skygging på etterfølgende gjenvekst ved 5-14 "C av kvekejordstengler og røtter av åkerdylle og åkertistel ble presentert.