Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2020
Forfattere
Eva Narten HøbergSammendrag
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Forfattere
Ryan Bright Micky Allen Clara Antón-Fernández Helmer Belbo Lise Dalsgaard Stephanie Eisner Aksel Granhus O. Janne Kjønaas Gunnhild Søgaard Rasmus AstrupSammendrag
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Sammendrag
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Forfattere
Milica Fotiric Akšic J. Mutic Z. Tešic Mekjell MelandSammendrag
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Forfattere
Jan-Peter George Jan Pisek Tobias Biermann Arnaud Carrara Edoardo Cremonese Matthias Cuntz Silvano Fares Giacomo Gerosa Thomas Grünwald Niklas Hase Michal Heliasz Andreas Ibrom Alexander Knohl Bart Kruijt Hikdeki Kobayashi Holger Lange Jean-Marc Limousin Denis Loustau Petr Lukes Riccardo Marzuoli Meelis Mölder Leonardo Montagnani Johan Neirynck Matthias Peichl Corinna Rebmann Marius Schmidt Francisco Ramon Lopez Serrano Kamel Soudani Caroline Vincke Roman ZweifelSammendrag
Det er ikke registrert sammendrag
Forfattere
Jan-Peter George Jan Pisek Tobias Biermann Arnaud Carrara Edoardo Cremonese Matthias Cuntz Silvano Fares Giacomo Gerosa Thomas Grünwald Niklas Hase Michal Heliasz Andreas Ibrom Alexander Knohl Bart Kruijt Hikdeki Kobayashi Holger Lange Jean-Marc Limousin Denis Loustau Petr Lukes Riccardo Marzuoli Meelis Mölder Leonardo Montagnani Johan Neirynck Matthias Peichl Corinna Rebmann Marius Schmidt Francisco Ramon Lopez Serrano Kamel Soudani Caroline Vincke Roman ZweifelSammendrag
Det er ikke registrert sammendrag
Forfattere
Hugo Moreno Victor Rueda-Ayala Angela Ribeiro Jose Bengochea-Guevara Juan López Gerassimos Peteinatos Constantino Valero Dionisio AndújarSammendrag
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Forfattere
Anita SønstebySammendrag
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Forfattere
Anita SønstebySammendrag
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Sammendrag
The stramenopile alga Nannochloropsis evolved by secondary endosymbiosis of a red alga by a heterotrophic host cell and emerged as a promising organism for biotechnological applications, such as the production of polyunsaturated fatty acids and biodiesel. Peroxisomes play major roles in fatty acid metabolism but experimental analyses of peroxisome biogenesis and metabolism in Nannochloropsis are not reported yet. In fungi, animals, and land plants, soluble proteins of peroxisomes are targeted to the matrix by one of two peroxisome targeting signals (type 1, PTS1, or type 2, PTS2), which are generally conserved across kingdoms and allow the prediction of peroxisomal matrix proteins from nuclear genome sequences. Because diatoms lost the PTS2 pathway secondarily, we investigated its presence in the stramenopile sister group of diatoms, the Eustigmatophyceae, represented by Nannochloropsis. We detected a full-length gene of a putative PEX7 ortholog coding for the cytosolic receptor of PTS2 proteins and demonstrated its expression in Nannochloropsis gaditana. The search for predicted PTS2 cargo proteins in N. gaditana yielded several candidates. In vivo subcellular targeting analyses of representative fusion proteins in different plant expression systems demonstrated that two predicted PTS2 domains were indeed functional and sufficient to direct a reporter protein to peroxisomes. Peroxisome targeting of the predicted PTS2 cargo proteins was further confirmed in Nannochloropsis oceanica by confocal and transmission electron microscopy. Taken together, the results demonstrate for the first time that one group of stramenopile algae maintained the import pathway for PTS2 cargo proteins. To comprehensively map and model the metabolic capabilities of Nannochloropsis peroxisomes, in silico predictions needs to encompass both the PTS1 and the PTS2 matrix proteome.