Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2025
Sammendrag
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Forfattere
Igor A. Yakovlev Thiago Inagaki Junbin Zhao Pierre-Adrien Rivier Hege Særvold Steen Inger Heldal Daniel Rasse Jihong Liu Clarke Nicholas ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Teresa Gómez de la Bárcena Tatiana Francischinelli Rittl Eva Farkas Daniel Rasse Cédric Plessis Helge Meissner Christophe Moni Trond Henriksen Randi Berland FrøsethSammendrag
Det er ikke registrert sammendrag
Sammendrag
Update on the progress from project AgriCascade
Sammendrag
Research Highlights The microbial community applied with the digestate accelerated methane formation. CO2 emission was higher under waterlogging than at field capacity in soil with low organic content. Waterlogging decreased methane emission in unamended soil. Only digestate where some methane potential was left induced high methane emission. Methanogens and methanotrophs abundances dynamics partly explain emission patterns.
Forfattere
Qiang Liu Mahmoud Mazarji Yeqing Li Lu Feng Hongjun Zhou Quan Xu Alastair James Ward Yajing Wang Jianping Su Chunming Xu Junting PanSammendrag
Humic acid (HA) is an inhibitor that can diminish bioconversion during anaerobic digestion (AD). In this study, a non-metallic nanomaterial, N-doped carbon quantum dots (NCQD), was synthesized to alleviate the HA inhibition, followed by exploring its mechanism. Adding 500 mg/L NCQD prevented HA inhibition by binding to HA or microorganism surfaces, thus avoiding contact between them. This phenomenon, known as shielding inhibition, involves the formation of hydrogen bonds and chemical bonds. The intermolecular force and dissociation constant (KD) between NCQD and HA were determined to be 112.83 ±3.98 nN and 1.0 ±0.07 ×10 7 M, respectively. NCQD could promote electron transfer in AD systems. According to the in-depth microbial analysis, NCQD significantly enhanced the metabolic pathways of methanogenesis and biosynthesis of coenzyme F420. This groundbreaking research demonstrates the dual functionality of NCQD by effectively shielding against HA inhibition and promoting electron transfer. The study also unravels the mechanism of interface interaction, electron transfer, and metabolic pathways, leading to significant breakthroughs in addressing HA inhibition.
Forfattere
Getachew Birhanu Abera Aryan Bhusal Thea Os Andersen Shuai Wang Nabin Aryal Svein Jarle Horn Lu FengSammendrag
In-situ biomethanation is an efficient process for converting carbon dioxide (CO2) to methane (CH4) using hydrogen (H2) alongside anaerobic digestion (AD) process. However, AD of protein rich substrate often leads to the accumulation of ammonia nitrogen at high concentration. As a major inhibitor, this accumulation affects not only the AD process but also in-situ biomethanation. This study investigated the impact of ammonia nitrogen (0.5–5 g/L) on biomethanation performance using anaerobic moving-bed biofilm reactors (AnMBBRs). Without biofilm/biocarrier support, methane production was significantly inhibited above 3 g/L of ammonia nitrogen. In contrast, AnMBBR maintained high methane yields of 156.5 NmL/Lreactor at 2.5 g/L and 151.3 NmL/Lreactor at 5 g/L ammonia nitrogen, representing increases of 49 % and 76 %, respectively, compared to reactors without biofilm. Microbial analysis via 16S rRNA sequencing showed that Methanothermobacter, a thermophilic hydrogenotrophic methanogen, increased in relative abundance under ammonia nitrogen stress, which was further supported by carbon isotope analysis. Overall, these results highlighted the potential of AnMBBR to overcome ammonia nitrogen stress in in-situ biomethanation.
Forfattere
Ya-Lan Hong Wei-Ming Xi Ya-Ting Wang Yi Yuan Zong-Zhuan Shen Ming Tian Jihong Liu Clarke Wang-Ying Xie Fang-jie ZhaoSammendrag
Environmental transmission of antibiotic resistance poses a significant threat to human health by undermining the efficacy of therapeutic interventions against bacterial infections. Agricultural practices, particularly the application of organic fertilizers derived from animal manure, are major contributors to the spread of antibiotic resistance determinants (ARDs) in soil ecosystems. However, the fates of ARDs and their bacterial hosts in soil following organic fertilization as well as the impact of water management regimes remain poorly understood. We investigated the attenuation and persistence of ARDs in soil following organic fertilization under water management practices of upland, continuous flooding, and intermittent flooding. Most ARDs introduced via the organic fertilizer exhibited significant attenuation, with half-lives ranging from 19 to 50 days, primarily due to the decline of fertilizer-derived bacterial hosts. Specific ARDs, such as aph(3’)-IIIa and tetO, persisted across all treatments. Upland conditions accelerated the attenuation of ARDs and their pathogenic hosts compared to f looding conditions, which prolonged their survival and promoted horizontal gene transfer. The divergent responses of ARD composition and soil bacterial communities to the environmental variables revealed a unique dissemination pattern, wherein the soil co-occurring bacterial communities served as critical hubs for the dissemination of ARDs and their bacterial hosts from organic fertilizers. The soil co-occurring bacterial communities exhibited strong interspecies interactions and high sensitivity to environmental changes. Targeted strategies to disrupt these assembly hubs may provide an effective way to mitigate the spread of antibiotic resistance from organic fertilizers to soil ecosystems.
Forfattere
Erik J. JonerSammendrag
Det er ikke registrert sammendrag
Forfattere
Ana-Maria Pantazica Alexander Hammel Iuliana Caras Irina Ionescu Catalin Tucureanu Adrian Onu Maria Murace Jihong Liu Clarke Crina Stavaru Norica Branza-Nichita Ralph BockSammendrag
Microalgae represent promising production factories for the light-driven, cost-effective production of recombinant proteins. The red microalga Porphyridium purpureum displays particularly favourable transgene expression properties due to the episomal maintenance of transformation vectors at high copy numbers in the nucleus. In this work, we explored the potential of Porphyridium purpureum to synthesise a candidate vaccine against Hepatitis B virus (HBV). We show high-yield expression of an HBV chimeric surface antigen and efficient assembly of virus-like particles (VLPs) in algal cells. We established a purification protocol for the VLPs and conducted vaccination studies in experimental animals. The results demonstrate that the alga-produced HBV antigen elicits superior humoral and cellular immune responses compared to a commercial HBV vaccine produced in yeast. The antigen triggers virus-neutralising antibodies against different HBV variants, including vaccine-escape mutations that evade the immune response to current vaccines in humans. Our work establishes Porphyridium as a highly promising production platform for vaccines and other proteinaceous biopharmaceuticals.