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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2023

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Sammendrag

Pyrolysis is a valid thermos-chemical process of energy production that produces biochar from potentially harmful biomasses. This study aims to investigate the pyrolytic conversion of olive mill solid residues (OMSR) into biochar, with the aim of characterizing this product towards applications for soil improvement and soil C sequestration. Production parameters of OMSR-biochar (OB) and physico-chemical characteristics were analyzed and compared with published data to assess the potential of OB to serve as a soil amendment and soil C sequestration method. The slow pyrolysis of OMSR at 450° leads to a good proportion between produced products (fuels liquid and gas, and solid), and generates about the 35% of OB. In turn, this product reveals the absence of phytotoxicity, the presence of exchangeable surface cations, structure, particle size distribution and external surface groups suitable for agricultural uses, and high C content with a potential long lasting in soil. The physico-chemical characteristics of OB reported here suggest that OB could be used for improving soils and increasing C sequestration in a sustainable way.

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Sammendrag

The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet this goal, the development of improved HBV antigens is critical to overcome non-responsiveness to standard vaccines based on the yeast-produced, small (S) envelope protein. We have recently shown that combining relevant immunogenic determinants of S and large (L) HBV proteins in chimeric antigens markedly enhances the anti-HBV immune response. However, the demand for cost-efficient, high-quality antigens remains challenging. This issue could be addressed by using plants as versatile and rapidly scalable protein production platforms. Moreover, the recent generation of plants lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO), by CRISPR/Cas9 genome editing, enables production of proteins with “humanized” N-glycosylation. In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric HBV S/L vaccine candidate produced in wild-type and FX-KO Nicotiana benthamiana. Prevention of β-1,2-xylose and α-1,3-fucose attachment to the HBV antigen significantly increased the immune response in mice, as compared with the wild-type plant-produced counterpart. Notably, the antibodies triggered by the FX-KO-made antigen neutralized more efficiently both wild-type HBV and a clinically relevant vaccine escape mutant. Our study validates in premiere the glyco-engineered Nicotiana benthamiana as a substantially improved host for plant production of glycoprotein vaccines.