Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2023
Forfattere
Hang Su Andre van Eerde Espen Rimstad Ralph Bock Norica Branza-Nichita Igor A. Yakovlev Jihong Liu ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Ana-Maria Madalina Pantazica Andre van Eerde Mihaela-Olivia Dobrica Iuliana Caras Irina Ionescu Adriana Costache Catalin Tucureanu Hege Særvold Steen Catalin Lazar Inger Heldal Sissel Haugslien Adrian Onu Crina Stavaru Norica Branza-Nichita Jihong Liu ClarkeSammendrag
The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet this goal, the development of improved HBV antigens is critical to overcome non-responsiveness to standard vaccines based on the yeast-produced, small (S) envelope protein. We have recently shown that combining relevant immunogenic determinants of S and large (L) HBV proteins in chimeric antigens markedly enhances the anti-HBV immune response. However, the demand for cost-efficient, high-quality antigens remains challenging. This issue could be addressed by using plants as versatile and rapidly scalable protein production platforms. Moreover, the recent generation of plants lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO), by CRISPR/Cas9 genome editing, enables production of proteins with “humanized” N-glycosylation. In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric HBV S/L vaccine candidate produced in wild-type and FX-KO Nicotiana benthamiana. Prevention of β-1,2-xylose and α-1,3-fucose attachment to the HBV antigen significantly increased the immune response in mice, as compared with the wild-type plant-produced counterpart. Notably, the antibodies triggered by the FX-KO-made antigen neutralized more efficiently both wild-type HBV and a clinically relevant vaccine escape mutant. Our study validates in premiere the glyco-engineered Nicotiana benthamiana as a substantially improved host for plant production of glycoprotein vaccines.
Forfattere
Volkmar Timmermann Isabella Børja Nicholas Clarke Rune Eriksen Jostein Gohli Gro Hylen Jane Uhd Jepsen Paal Krokene Holger Lange Helge Meissner Nina Elisabeth Nagy Jørn-Frode Nordbakken Sverre Solberg Halvor Solheim Ole Petter Laksforsmo Vindstad Bjørn Økland Wenche AasSammendrag
Source at <a href=https://www.nibio.no/>https://www.nibio.no/</a>.
Sammendrag
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Forfattere
Thiago Inagaki Angela R. Possinger Steffen A. Schweizer Carsten W. Mueller Carmen Hoeschen Michael J. Zachman Lena F. Kourkoutis Ingrid Kögel-Knabner Johannes LehmannSammendrag
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Forfattere
Junbin Zhao Mikhail Mastepanov Cornelya Klutsch Tobias Daugaard-Petersen Hanna Marika SilvennoinenSammendrag
Det er ikke registrert sammendrag
Forfattere
Eva FarkasSammendrag
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2022
Forfattere
Jogeir N. Stokland Kjersti Holt Hanssen Delphine Derrien Bernhard Zeller Alice Budai Daniel RasseSammendrag
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Forfattere
Alice BudaiSammendrag
Det er ikke registrert sammendrag
Forfattere
Liang Wang Maria N.P. Olsen Christophe Moni Alba Diuegez-Alonso Jose Maria de la Rosa Marianne Stenrød Xingang Liu Liangang MaoSammendrag
Det er ikke registrert sammendrag