Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2008
Sammendrag
Most fungicide applications targeting apple scab aim to control primary infections caused by ascospores and spraying is thereby linked to ascospore availability. We investigated the effect of pre bud break climatic conditions on seasonal patterns of ascospore release. Apple leaves bearing pseudothecia of Venturia inaequalis were overwintered at orchard sites in 8 countries for up to 3 years. Leaf samples were collected 2 to 5 weeks before bud break and again at bud break, air dried, and sent via airmail to Norway. The samples were stored at -18 ºC upon arrival until tested. Disks cut from each replicate leaf sample were incubated moist at 20 ºC to allow ascospore maturation but prevent discharge. Matured ascospores were induced to discharge twice a week and enumerated until the supply was exhausted. The proportion of ascospores ejected was fitted against degree day accumulation using logistic regression. The regression intercept (onset maturation), slope (maturation rate), as well as the absolute number of spores counted differed significantly (P < 0.001, P = 0.05, P < 0.001 respectively) among sites and sampling dates. There was a significant interaction between site and sampling date, indicating that climatic conditions prior to bud break differentially impacted the subsequent ascospore availability. Observed differences could perhaps be used to further refine previously described models of ascospore maturity.
Forfattere
Arne Stensvand Maria Herrero Håvard Eikemo Andrew Dobson David M. Gadoury Robert C. Seem Catherine Heidenreich Mary Jean WelserSammendrag
Artikkelen gir ein kort omtale av resultat frå eit forskingsprosjekt om mjøldogg i jordbær.
Sammendrag
Incomplete combustion during vegetation fire can lead to the conversion of plant and soil organic matter (OM) into charcoal. The thermally altered OM is considered to contribute to the stable pool of soil C. Most of the data on thermal alteration of plant material were obtained in the laboratory, whereas fire consequences on ecosystem C storage calls for data collected in natural-fire conditions. The objective of this study was to relate the quality of visually-identified litter charcoal and the temperature recorded during a scrubland prescribed fire. Litter was sampled before and after the fire along a transect in the 30 ha experimental site. Litter-size fractions were analyzed for chemical composition and properties by elementary and isotopic analysis, solid-state 13C nuclear magnetic resonance spectroscopy, differential scanning calorimetry and quantification of oxidation-resistant pyrogenic C. The maximum temperature reached within the litter layer during fire was assessed with thermo-sensitive paints. Our results showed that fire had little effect on bulk litter composition because the fire event induced a large litter fall of both charred and non-charred material, resulting in the impossibility to distinguish new-litter-input and charring processes. As a consequence, the visual identification and separation of burned and unburned material constituted an essential preliminary step for chemical characterization of thermally altered organic matter. Fire temperatures ranged from 370 to 650°C. Charring signifi- cantly increased the litter C concentration by 115 to 142 mg g"1 under the effects of dehydration and aromatization processes occurring above 370°C. A significant correlation appeared between the production of aromatic structures, the decrease of O-alkyl C contribution and the temperature. The relationship between the maximum temperatures reached during the natural fire and the chemical transformation of the litter organic matter appeared highly consistent with previous results obtained under controlled conditions. Heating also led to a significant decrease of the 13C that we interpret as a higher thermal sensitivity of 13C-rich molecules. The elemental composition, NMR and thermal spectra are consistent with the low oxidation-resistant C concentration of this natural charcoal (16±5 % OC), reflecting a low condensation degree compared to graphitic-like model. These findings suggest that leaf-derived charcoal produced during natural vegetation fire may have a lower C storage potential than previously assumed.
Sammendrag
Vegetation fire is the worldwide disturbance that affects the largest area and biggest biomes variety. Fire instantaneously generates large C fluxes to the atmosphere, as gas and soot particles. In the same time, part of ecosystem organic matter (OM) is converted into charred material that may contribute to the stable pool of soil organic carbon (SOC). The net effect of vegetation fire on C sequestration remains uncertain because the two major impacts operate at very different timescales and C budget is highly dependent on ecosystem and fire conditions. The aim of the present research was to assess fire-induced C fluxes to the atmosphere and as new litter and charcoal production during a prescribed fire in a subtropical oak shrub. Pre-fire biomass and post-fire charred and unburned biomass were determined for vegetation leaves and stems, litter and soil in 20 sub-plots installed in a 30-ha area prescribed for fire. Concentrations of C were determined, and fluxes among pools and to the atmosphere were derived from these measurements. In a first assessment, charred OM was visually identified in standing biomass and litter using its black and shiny aspect. In a second step, a strong chemical oxidation with K2Cr2O7/H2SO4 was used to isolate only a highly recalcitrant part of pyrogenic C. After the fire, standing dead biomass was only composed of stems with charred surface. The leaves transferred from vegetation to litter during the fire represented more than a half of post-fire litter. Percentage of initial C pool that was lost to the atmosphere as gas or particles was 55 % from vegetation stems, 80 % from vegetation leaves, and 70 % from litter. Soil C stocks were not significantly modified by fire, in agreement with moderate temperature elevation in the soil proper. Total C release to the atmosphere, including gas and particles, was 2.6 kg C m"2. Visually-identified charcoal represented 5% of remaining stem C (i.e. 60 g C m"2) and 21% of post-fire litter C (i.e. 80 g C m"2). The stem and litter charcoal contained 4±4 % and 16±5 % of highly recalcitrant C, respectively. We assessed that a typical scrubland fire may add between 10 and 140 g C m"2of chemically stable pyrogenic C to the soil. The conversion rate of ecosystem C to chemically stable pyrogenic C would be between 0.2 and 3.4 %.
Forfattere
Ricardo Holgado Anette Stryken Christer Magnusson Irene Rasmussen Kari-Ann Strandenæs Bonsak HammeraasSammendrag
A survey was undertaken to investigate the occurrence of plant parasitic nematodes in the municipality of Lier located in southern Norway. A wide variety of vegetables crops are grown in this area. Soils in Lier are mostly sandy loam, and irrigation is practiced when necessary during the growing season. There is little information on the occurrence on plant parasitic nematodes in vegetables; particularly in areas where farmers have specialized in intensive production, and often grow two to three cultures of vegetables during the growing season. In the year prior to this investigation several crops had been observed with symptoms of attack by plant parasitic nematodes. The aim of this survey was to identify the species responsible for the damage. The emphasis was placed on genera of known economic importance. During the end of the growing season plants showing poor growth or symptoms of nematode damage and plants with healthy appearance were sampled. A total of 74 root samples were collected and assessed for galls and 37 soil samples were collected from the rhizospheres. In the study samples were collected from 7 producers. Five samples were taken from cauliflower (Brassica oleraceae var. botrytis), 4 from broccoli (Brassica oleraceae var. italica), 8 from pak-choy Chinese cabbage (Brassica rapa ssp. pekinensis), 8 from lettuce (Lactuca sativa) and 1 from celery (Apium graveolens). Soil sampling included 11 samples from areas with fallow, ploughed and rotocultivated land. Root knot nematodes were not recorded in the root samples. In soil samples economically important nematode genera were recorded. Tylenchorhynchus sp. (72.9 % of samples), was the most frequently encountered genus, followed by the species Heterodera cruciferae and other Heterodera spp. (67.5 %), Pratylenchus spp. (54 %), Paratylenchus spp. (29,7 %), and Helicotylenchus spp. (8.1 %). In addition to cyst detections Heterodera juveniles were found in 32.4 % of samples. In all the samples from Pak-choy Chinese cabbage (Brassica rapa ssp. pekinensis) Heterodera cruciferae was recorded, symptoms of nematode damage was also observed in the field. The results of the survey will help in planning future research as well as in developing effective nematode management strategies suitable for vegetable producer particularly in this area. The authors are thankful to the FMLA in Buskerud County for economical support.
Forfattere
Ricardo Holgado Anette Stryken Christer Magnusson Irene Rasmussen Kari-Ann Strandenæs Bonsak HammeraasSammendrag
A survey was undertaken to investigate the occurrence of plant parasitic nematodes in the municipality of Lier located in southern Norway. A wide variety of vegetables crops are grown in this area. Soils in Lier are mostly sandy loam, and irrigation is practiced when necessary during the growing season. There is little information on the occurrence on plant parasitic nematodes in vegetables; particularly in areas where farmers have specialized in intensive production, and often grow two to three cultures of vegetables during the growing season. In the year prior to this investigation several crops had been observed with symptoms of attack by plant parasitic nematodes. The aim of this survey was to identify the species responsible for the damage. The emphasis was placed on genera of known economic importance. During the end of the growing season plants showing poor growth or symptoms of nematode damage and plants with healthy appearance were sampled. A total of 74 root samples were collected and assessed for galls and 37 soil samples were collected from the rhizospheres. In the study samples were collected from 7 producers. Five samples were taken from cauliflower (Brassica oleraceae var. botrytis), 4 from broccoli (Brassica oleraceae var. italica), 8 from pak-choy Chinese cabbage (Brassica rapa ssp. pekinensis), 8 from lettuce (Lactuca sativa) and 1 from celery (Apium graveolens). Soil sampling included 11 samples from areas with fallow, ploughed and rotocultivated land. Root knot nematodes were not recorded in the root samples. In soil samples economically important nematode genera were recorded. Tylenchorhynchus sp. (72.9 % of samples), was the most frequently encountered genus, followed by the species Heterodera cruciferae and other Heterodera spp. (67.5 %), Pratylenchus spp. (54 %), Paratylenchus spp. (29,7 %), and Helicotylenchus spp. (8.1 %). In addition to cyst detections Heterodera juveniles were found in 32.4 % of samples. In all the samples from Pak-choy Chinese cabbage (Brassica rapa ssp. pekinensis) Heterodera cruciferae was recorded, symptoms of nematode damage was also observed in the field. The results of the survey will help in planning future research as well as in developing effective nematode management strategies suitable for vegetable producer particularly in this area. The authors are thankful to the FMLA in Buskerud County for economical support.
Forfattere
Arvid Steen Turid Strøm Aksel BernhoftSammendrag
Background Selenium is part of the antioxidant defence system in animals and humans. The available selenium concentration in soil is low in many regions of the world. The purpose of this study was to evaluate the effect of organic versus inorganic selenium supplementation on selenium status of ewes, their lambs, and slaughter lambs. Methods Ewes on four organic farms were allocated five or six to 18 pens. The ewes were given either 20 mg/kg inorganic selenium as sodium selenite or organic selenium as selenized nonviable yeast supplementation for the two last months of pregnancy. Stipulated selenium concentrations in the rations were below 0.40 mg/kg dry matter. In addition 20 male lambs were given supplements from November until they were slaughtered in March. Silage, hay, concentrates, and individual ewe blood samples were taken before and after the mineral supplementation period, and blood samples were taken from the newborn lambs. Blood samples from ewes and lambs in the same pens were pooled. Muscle samples were taken from slaughter lambs in March. Selenium concentrations were determined by atomic absorption spectrometry with a hydride generator system. In the ANOVA model, selenium concentration was the continuous response variable, and selenium source and farm were the nominal effect variables. Two-sample t-test was used to compare selenium concentrations in muscle samples from the slaughtered lambs that received either organic or inorganic selenium supplements. Results In all ewe pens the whole blood selenium concentrations increased during the experimental period. In addition, ewe pens that received organic selenium had significantly higher whole blood selenium concentrations (mean 0.28 "g/g) than ewe pens that received inorganic selenium (mean 0.24 "g/g). Most prominent, however, was the difference in their lambs; whole blood mean selenium concentration in lambs from mothers that received organic selenium (mean 0.27 "g/g) was 30% higher than in lambs from mothers that received inorganic selenium (mean 0.21 "g/g). Slaughter lambs that received organic selenium had 50% higher meat selenium concentrations (mean 0.12 mg/kg wet weight) than lambs that received inorganic selenium (mean 0.08 mg/kg wet weight). Conclusion Organic selenium supplementation gave higher selenium concentration in ewe and newborn lamb blood and slaughter lamb meat than inorganic selenium supplementation.
Sammendrag
In April 2007, orange-red pustules were found in needle scars on defoliated, dead shoots on a nordmann fir (Abies nordmanniana) Christmas tree in Rogaland County in south western Norway. A microscope slide made from the pustules revealed Fusarium-macrospores. On average they were 40.2 µm long and 2.9 µm wide (n=50). No microspores were found. A red coloured culture was obtained by transferring spore mass from a sporodochium to PDA (potato dextrose agar) with a sterile needle. The culture was transferred to SNA (spezieller nährstoffarmer agar) where macrospores developed. The majority of the spores had three septa, most of them were slightly curved, apically bent, basal cells were foot shaped, and spores were long and slender. Six singlespore cultures appeared identical on SNA. Two were chosen for an inoculation test. This test took place on 4 June 2007 in a polyethylene tunnel where the plants were kept during the whole experimental period. Nearly fully grown current year shoots on container grown nordmann fir transplants were inoculated by placing PDA agar plugs with fungal growth at the base of some unwounded needles on each shoot. Twelve transplants were used for each of the two isolates. The plants were covered with polyethylene bags for five days. After the bags were removed, the foliage was kept wet throughout the growing season by 1-3 minutes (depending on temperature) overhead irrigation every third hour. Brown shoots developed during the summer regardless of which singlespore isolate that had been used for inoculation. Samples from brown shoots were collected 20 October. No sporodochia, like we found under field conditions in April, had developed on the inoculated shoots. Neither did they develop after incubation in the laboratory. Only a whitish, fluffy mycelium was seen. CZID (Czapek dox iprodione dichloran agar) was used for reisolation from the dead shoots. From each group of 12 inoculated plants, two and three Fusarium-cultures were obtained, respectively. No symptoms were observed and no Fusarium sp. was isolated from the control plants (exposed to same treatment, but agar plugs with no fungal growth had been used). The Fusarium strain could not be accurately identified to species when the FUSARIUM-ID database v. 1.0 (http://fusarium.cbio.psu.edu) was searched with partial sequence of TEF (translation elongation factor 1-"). The original culture plus the two single spore cultures used for inoculation had identical TEF sequences which were most similar to F. acuminatum (97% identity). One out of six single spore cultures from each of the five reisolates, was sequenced. One of them matched the original culture, but the other four were most similar to F. avenaceum (98% identity). Different from the original culture, the F. av.-like isolates had both macro- and microconidia (0-3 septa, oval spore shape). On PDA, the F. ac.-like culture grew 25 mm in 72 hours (dark and 25°C) and the four F. av.-like cultures grew between 32 to 40 mm. They could not be distinguished by colour. Hyphal coils were found on both species, but were most prominent on the F. ac.-like culture. The fact that sequencing revealed involvement of two Fusarium-species, makes is difficult to draw conclusions about pathogenicity, but since the control plants showed no symptoms, there may be indications that the F. ac.-like fungus killed the inoculated shoots and that the F. av.-like fungus came in as a secondary invader from the environment inside or outside the tunnel. F. acuminatum is reported as a pathogen on conifer seeds and seedlings, but to our knowledge this is the first report of a F. ac.-like fungus causing dieback on a Christmas tree.
Sammendrag
Extensive damage by the needle parasites Delphinella abietis and Herpotrichia parasitica (Herpotrichia Needle Browning) was recently observed at the west coast of Norway. D. abietis was by far the most widespread, but once established in the field, the damage potential seemed to be equally high for both pathogens. D. abietis occurred on subalpine fir (Abies lasiocarpa), Turkish fir (A. bornmuelleriana), Siberian fir (A. sibirica), and nordmann fir (A. nordmanniana) in Christmas tree plantations, and minor damage was seen on Noble fir (A. procera) for bough production. H. parasitica was found on Turkish fir and nordmann fir in Christmas tree plantations. None of the diseases are new to Abies spp. in Norway, but they have not previously been reported from Christmas tree fields.
Sammendrag
To forskjellige strategier for fôring av ammekyr i inneperioden ble utprøvd ved Bioforsk Nord Tjøtta sitt storfefjøs gjennom to forsøksperioder, fra november 2006 til november 2008. Restriktiv gruppe ble fôret til å redusere kroppsholdet med 1 -1,5 holdpoeng (utnyttelse av kroppsreservene), mens normgruppen ble fôret til vedlikehold for ammekyr på ca 550 kg levendevekt. Kroppshold på kyrne ved innsett lå i overkant av 4, hvor tilrådningene er 3,0-3,5. Det opplevdes som vanskelig å fôre kyrne strengt restriktivt, da de viste atferdsendring som rastløshet og brøling når de ikke hadde nok fôrtilgang. Fôringsregimet måtte derfor justeres noe gjennom perioden av hensyn til bl.a. dyrevelferd. Holdet på kyrne ble ikke redusert i den grad som forventet i noen av forsøksperiodene. Det ble ikke funnet noen signifikant forskjell på kalvenes fødselsvekt, utslippsvekt, høstvekt og tilvekst inne og på beite mellom gruppene. Tilvekst hos kalvene i inneperioden var tilfredsstillende begge år og det opptrådte ingen sjukdom som følge av forsøket noen av årene. Fôringsforsøket viste at strategien med å fôre restriktivt for å spare vinterfôr og utnytte kroppsreservene til et visst nivå kan gi tilfredsstillende produksjonsresultater i form av fruktbarhet, kalvetilvekst og kalvehelse. Det må i et slikt fôringsopplegg tas hensyn til dyrevelferden, ved at kyrne får tilfredsstilt sitt behov for vomaktivitet og tidsfordriv gjennom hele døgnet. Dette kan løses ved å gi lavenergifôr i større mengder (for eksempel ubehandlet halm eller seint høstet gras), slik at rasjonen strekkes mer utover dagen.