Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2011
Authors
Emmanuel Lapied Johanne Y. Nahmani Elara Moudilou Perrine Chaurand Jérôme Labille Jérôme Rose Jean-Marie Exbrayat Deborah Helen Oughton Erik J. JonerAbstract
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Abstract
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Authors
Erik J. JonerAbstract
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2010
Authors
Hsin-Ho Huang Daniel Camsund Peter Lindblad Thorsten HeidornAbstract
Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like P(rnpB) and variants of P(rbcL), and a version of the promoter P(trc) with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
Abstract
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Authors
Ove BergersenAbstract
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Abstract
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Abstract
The efficiency of different organic waste material as NPK fertilizer and risk for leaching losses related to shower precipitation in the first part of the growing season was testet in a pot experiment on a sandy soil in green house. Six organic fertilizers were evaluated: liquid anaerobic digestate (LAD) of source separated household waste, nitrified liquid anaerobic digestate (NLAD) of same origin as LAD, meat and bone meal (MBM), hydrolysed salmon protein (HSP), reactor composted catering waste (CW) and cattle manure (CM). Unfertilized control, calcium nitrate (CN) and compound fertilizer, Fullgjødsel® 21-4-10 were used as reference fertilizers. Two levels of N-fertilization were applied: 80 kg N ha-1 and 160 kg N ha-1. The amount of fertilizer applied was based on content of mineral N for LAD, NLAD, CN and Fullgjødsel, while Kjeldahl-N content was used for dosage of MBM, HSP, CW and CM. At Zadoks 14 the pots were given a surplus of 28 mm water, as a simulated shower precipitation, and leached water was collected and analyzed for content of N and P. LAD and Fullgjødsel® gave equal yield of barley and uptake of N, P, and K in barley grain, when equal amounts of mineral nitrogen were applied. NLAD gave significantly lower barley yield than the original LAD due to leaching of nitrate-N after simulated surplus of 28 mm precipitation at Zadoks 14. CW also gave yield of barley grain similar to Fullgjødsel®, but significantly less yield of straw. The nutrient content in the different organic fertilizers caused different yield limiting effects. MBM showed K deficiency and had equally small K uptake as CN. Cattle manure had only a small portion of mineral N, and low uptake of N. NLAD had low uptake of P compared to LAD, which also was related to smaller amount of P applied in NLAD. The was significant increased leaching of nitrate N from the treatments receiving 160 kg N ha-1 of CN and NLAD compared to all the other organic fertilizers. It was found significant increased leaching of NH4-N at LAD with 160 kg N ha-1 compared to the other treatments, but the amount of leached NH4-N was very small compared to the nitrate-N leaching for the CN and NLAD treatments. Although the LAD treatment received less P than the CM treatment, the highest P-leaching was found for the LAD treatment. A relatively high proportion of the leached P was PO4-P for the LAD treatment receiving 160 kg N ha-1. CM and CW also had significantly higher P leaching than the other organic fertilizers at 160 kg N ha-1, while most of the treatments had very small P losses and not significantly higher than the unfertilized control. This study showed that liquid anaerobic digestate (LAD) was equally good as NPK fertilizer to barley when equal amounts of mineral N were applied. Liquid anaerobic digestate made of source separated household waste can be recommended as fertilizer to cereals. Nitrification of the ammonium N in the digestate caused significantly increased nitrate leaching, and can not be recommended. The composted catering (CW) and hydrolysed salmon protein (HSP) also showed good fertilizer effect, but these fertilizers had not optimal NPK composition and had lower K content than the crop demand. In these materials are used as fertilizers additional K should be applied in order to obtain normal yields.
Authors
laurice Cecillion Daniel Rasse Katrin Knoth de Zarruk Alice Budai Roald Sørheim R Janotte G Ragan E Ancelet F Bray JJ Brun S D Danieli C E Thompsson A F Plante G HudsonAbstract
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Authors
Trine EggenAbstract
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