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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2019

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Sammendrag

Purpose of Review The adoption of Structure from Motion photogrammetry (SfM) is transforming the acquisition of three-dimensional (3D) remote sensing (RS) data in forestry. SfM photogrammetry enables surveys with little cost and technical expertise. We present the theoretical principles and practical considerations of this technology and show opportunities that SfM photogrammetry offers for forest practitioners and researchers. Recent Findings Our examples of key research indicate the successful application of SfM photogrammetry in forestry, in an operational context and in research, delivering results that are comparable to LiDAR surveys. Reviewed studies have identified possibilities for the extraction of biophysical forest parameters from airborne and terrestrial SfM point clouds and derived 2D data in area-based approaches (ABA) and individual tree approaches. Additionally, increases in the spatial and spectral resolution of sensors available for SfM photogrammetry enable forest health assessment and monitoring. The presented research reveals that coherent 3D data and spectral information, as provided by the SfM workflow, promote opportunities to derive both structural and physiological attributes at the individual tree crown (ITC) as well as stand levels. Summary We highlight the potential of using unmanned aerial vehicles (UAVs) and consumer-grade cameras for terrestrial SfM-based surveys in forestry. Offering several spatial products from a single sensor, the SfM workflow enables foresters to collect their own fit-for-purpose RS data. With the broad availability of non-expert SfM software, we provide important practical considerations for the collection of quality input image data to enable successful photogrammetric surveys.

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High-throughput sequencing is increasingly favoured to assay the presence and abundance of microRNAs (miRNAs) in biological samples, even from low RNA amounts, and a number of commercial vendors now offer kits that allow miRNA sequencing from sub-nanogram (ng) inputs. Although biases introduced during library preparation have been documented, the relative performance of current reagent kits has not been investigated in detail. Here, six commercial kits capable of handling <100ng total RNA input were used for library preparation, performed by kit manufactures, on synthetic miRNAs of known quantities and human total RNA samples. We compared the performance of miRNA detection sensitivity, reliability, titration response and the ability to detect differentially expressed miRNAs. In addition, we assessed the use of unique molecular identifiers (UMI) sequence tags in one kit. We observed differences in detection sensitivity and ability to identify differentially expressed miRNAs between the kits, but none were able to detect the full repertoire of synthetic miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the relative levels of the majority of miRNAs. UMI tags, at least within the input ranges tested, offered little advantage to improve data utility. In conclusion, biases in miRNA abundance are heavily influenced by the kit used for library preparation, suggesting that comparisons of datasets prepared by different procedures should be made with caution. This article is intended to assist researchers select the most appropriate kit for their experimental conditions.

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Root and butt-rot (RBR) has a significant impact on both the material and economic outcome of timber harvesting, and therewith on the individual forest owner and collectively on the forest and wood processing industries. An accurate recording of the presence of RBR during timber harvesting would enable a mapping of the location and extent of the problem, providing a basis for evaluating spread in a climate anticipated to enhance pathogenic growth in the future. Therefore, a system to automatically identify and detect the presence of RBR would constitute an important contribution in addressing the problem without increasing workload complexity for the machine operator. In this study we developed and evaluated an approach based on RGB images to automatically detect tree-stumps and classify them as to the absence or presence of rot. Furthermore, since knowledge of the extent of RBR is valuable in categorizing logs, we also classify stumps to three classes of infestation; rot = 0%, 0% < rot < 50% and rot >= 50%. In this work we used deep learning approaches and conventional machine learning algorithms for detection and classification tasks. The results showed that tree-stumps were detected with precision rate of 95% and recall of 80%. Using only the correct output (TP) of the stump detector, stumps without and with root and butt-rot were correctly classified with accuracy of 83.5% and 77.5%. Classifying rot to three classes resulted in 79.4%, 72.4% and 74.1% accuracy for stumps with rot = 0%, 0% < rot < 50% and rot >= 50\%, respectively. With some modifications, the algorithm developed could be used either during the harvesting operation to detect RBR regions on the tree-stumps or as a RBR detector for post-harvest assessment of tree-stumps and logs.

Sammendrag

The objective of this study was to assess the use of unmanned aerial vehicle (UAV) data for modelling tree density and canopy height in young boreal forests stands. The use of UAV data for such tasks can be beneficial thanks to the high resolution and reduction of the time spent in the field. This study included 29 forest stands, within which 580 clustered plots were measured in the field. An area-based approach was adopted to which random forest models were fitted using the plot data and the corresponding UAV data and then applied and validated at plot and stand level. The results were compared to those of models based on airborne laser scanning (ALS) data and those from a traditional field-assessment. The models based on UAV data showed the smallest stand-level RMSE values for mean height (0.56 m) and tree density (1175 trees ha−1 ). The RMSE of the tree density using UAV data was 50% smaller than what was obtained using ALS data (2355 trees ha−1 ). Overall, this study highlighted that the use of UAVs for the inventory of forest stands under regeneration can be beneficial both because of the high accuracy of the derived data analytics and the time saving compared to traditional field assessments.

Sammendrag

This paper describes the development and utility of the Norwegian forest resources map (SR16). SR16 is developed using photogrammetric point cloud data with ground plots from the Norwegian National Forest Inventory (NFI). First, an existing forest mask was updated with object-based image analysis methods. Evaluation against the NFI forest definitions showed Cohen's kappa of 0.80 and accuracy of 0.91 in the lowlands and a kappa of 0.73 and an accuracy of 0.96 in the mountains. Within the updated forest mask, a 16×16 m raster map was developed with Lorey's height, volume, biomass, and tree species as attributes (SR16-raster). All attributes were predicted with generalized linear models that explained about 70% of the observed variation and had relative RMSEs of about 50%. SR16-raster was segmented into stand-like polygons that are relatively homogenous in respect to tree species, volume, site index, and Lorey's height (SR16-vector). When SR16 was utilized in a combination with the NFI plots and a model-assisted estimator, the precision was on average 2–3 times higher than estimates based on field data only. In conclusion, SR16 is useful for improved estimates from the Norwegian NFI at various scales. The mapped products may be useful as additional information in Forest Management Inventories.