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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2016

Sammendrag

I 2016 er det utført 6 forsøk med 8 kjemiske skadedyrmidler, 2 biologiske skadedyrmidler, 2 feromonpreparater og 5 vekstregulerende preparater. Effekten er undersøkt mot kålmøll i kål,bladlus i erter, ulike viklerarter i eple, vanlig pæresuger i pære, og for vekstregulering i sommerblomster. Preparatene hadde varierende virkning og det vurderes om forsøkene skal videreføres. Det er også et pågående forsøk mot gransnutebille i skogplanteskole fra 2015 som avsluttes og rapporteres i 2017.

Sammendrag

Aphids in cereals are an important problem in Europe. Entomopathogenic fungi in the Phylum Entomophthoromycota are among their natural enemies. Under certain conditions, they can cause epizootic events and control pest aphid populations. This epizootic development is affected by many abiotic and biotic factors such as aphid species and their host plant (including weeds within the crop), fungal species and isolates, and temperature. Studies from Denmark, UK, Slovakia and suggest that the genus Pandora is the most prevalent fungal pathogen of the English grain aphid (Sitobion avenae). Which fungal species that is the most prevalent in populations of the other important aphid species in cereals in Europe, the Bird cherry-oat aphid (Rhopalosiphum padi), is less clear. We chose, however, to use Pandora to assess the biological control potential of Entomophthoromycota against aphids in cereals and to produce data that might be used in a pest-warning model incorporating the effect of this natural enemy. This was done by conducting laboratory studies on the virulence of two Pandora isolates (collected in the same field) on R. padi and Myzus persicae at three temperatures (12, 15 and 18◦C). M. persicae is a polyphagous aphid that may be present on weeds. It can be an alternative host for Pandora and hence might also affect the epidemic development of Pandora in aphids that are cereal pests. Our preliminary results show that R. padi is more resistant to the tested Pandora isolates than M. persicae. The two Pandora isolates had different virulence in the two aphid species tested. The temperature did not influence the virulence.

Sammendrag

This contribution demonstrates an example of experimental automatic image analysis to detect spores prepared on microscope slides derived from trapping. The application is to monitor aerial spore counts of the entomopathogenic fungus Pandora neoaphidis which may serve as a biological control agent for aphids. Automatic detection of such spores can therefore play a role in plant protection. The present approach for such detection is a modification of traditional manual microscopy of prepared slides, where autonomous image recording precedes computerised image analysis. The purpose of the present image analysis is to support human visual inspection of imagery data – not to replace it. The workflow has three components: • Preparation of slides for microscopy. • Image recording. • Computerised image processing where the initial part is, as usual, segmentation depending on the actual data product. Then comes identification of blobs, calculation of principal axes of blobs, symmetry operations and projection on a three parameter egg shape space.

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Sammendrag

A controlled climatic chamber microcosm experiment was conducted to examine how light affects the hourly sporulation pattern of the beneficial mite pathogenic fungus Neozygites floridana during a 24 h cyclus over a period of eight consecutive days. This was done by inoculating two-spotted spider mites (Tetranychus urticae) with N. floridana and placing them on strawberry plants for death and sporulation. Spore (primary conidia) discharge was observed by using a spore trap. Two light regimes were tested: Plant growth light of 150 μmol m−2 s−1 for 12 h supplied by high pressure sodium lamps (HPS), followed by either; (i) 4 h of 50 μmol m−2 s−1 light with similar HPS lamps followed by 8 h darkness (full HPS light + reduced HPS light + darkness) or (ii) 4 h of 50 μmol m−2 s−1 red light followed by 8 h darkness (full HPS light + red light + darkness). A clear difference in hourly primary conidia discharge pattern between the two different light treatments was seen and a significant interaction effect between light treatment and hour in day during the 24 h cycle was observed. The primary conidia discharge peak for treatment (ii) that included red light was mainly reached within the red light hours (19:00–23:00) and the dark hours (23:00–07:00). The primary conidia discharge peak for treatment (i) with HPS light only was mainly reached within the dark hours (23:00–07:00).

Sammendrag

With the Directive 2009/128/EC on sustainable use of pesticides, reductions in herbicide use is a European target. The aim of this study was to compare the fi eld-specifi c herbicide use resulting from simulated integrated weed management (IWM) with farmer’s actual use. Two IWM tools applicable for cereals were explored: VIPS – a web-based decision support system, and DAT sensor – a precision farming technology for patch spraying. VIPS (adaptation of Danish “Crop Protection Online”) optimizes herbicide – and dose to weed species densityand growth stage (including ALS-herbicide resistant populations), temperature, expected yield, cereal species- and growth stage. Weeds were surveyed (0.25 m2, n=23-31) prior to post-emergence spraying in spring 2013 (six fi elds) and 2014 (eight fi elds). DAT sensor enables automatic patch spraying of annual weeds within cereals. It consists of an RGB camera and custom-made image analysis. DAT sensor acquired more than 900 images (0.06 m2) per fi eld. Threshold for simulated patch spraying was relative weed cover (weed cover/ total vegetation cover) = 0.042. Treatment frequency index (TFI, actual dose/maximum approved dose summed for all herbicides) was calculated. Without resistance strategy, average TFI for VIPS was higher for winter wheat (0.96) than for spring cereals (0.38). Spring cereal fi elds with resistance strategies gave an average TFI of 1.45. Corresponding TFI for farmer’s applications were 1.40, 0.90 and 1.26, respectively. For one fi eld wherein both tools were explored in 2013 and 2014, TFI values for VIPS were 1.86 and 1.50 due to resistant Stellaria media, while TFI for farmer’s sprayings were around 1.00. DAT sensor simulated herbicide savings of 69% and 99%, corresponding to TFI values of 0.58 and 0.01, respectively. As measured by TFI, DAT sensor showed a higher potential in herbicide savings than VIPS. VIPS is available without costs to end-users today, while DAT sensor represents a future tool.

Sammendrag

The two-spotted spider mite, Tetranychus urticae, is a serious pest of numerous crops worldwide. Sustainable management solutions for T. urticae include predators and entomopathogens. Neozygites floridana is a naturally occurring obligate fungal pathogen of T. urticae and can cause declines in T. urticae populations. The purpose of this study was to determine whether releasing the predatory mite Phytoseiulus persimilis into T. urticae populations has the potential to increase transmission of N. floridana and accelerate the development of an epizootic. This is the first study quantifying the effect of P. persimilis on transmission of N. floridana to T. urticae in a controlled microcosm study. Our results show that introducing P. persimilis into T. urticae, populations increased the proportion of T. urticae infected with N. floridana. By the final sampling occasion, the number of T. urticae in the treatment with both the predator and the pathogen had declined to zero in both experiments, while in the fungus-only treatment T. urticae populations still persisted. We suggest that releasing P. persimilis into crops in which N. floridana is naturally present has the potential to improve spider mite control more than through predation alone.