Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Forfattere
Jihong Liu ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Jihong Liu ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Jihong Liu ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Ning Wang Huan Peng Shi-ming Liu Wen-kun Huang Ricardo Holgado Jihong Liu Clarke De-liang PengSammendrag
Soybean cyst nematode (SCN, Heterodera glycines (I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes (Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The qRT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles (J2s) were exposed to the Gram-positive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.
Forfattere
Fen Qiao Ling-An Kong Huan Peng Wen-Kun Huang Du-Qing Wu Shi-Ming Liu Jihong Liu Clarke De-Wen Qiu De-Liang PengSammendrag
Cereal cyst nematode (CCN, Heterodera avenae) presents severe challenges to wheat (Triticum aestivum L.) production worldwide. An investigation of the interaction between wheat and CCN can greatly improve our understanding of how nematodes alter wheat root metabolic pathways for their development and could contribute to new control strategies against CCN. In this study, we conducted transcriptome analyses of wheat cv. Wen 19 (Wen19) by using RNA-Seq during the compatible interaction with CCN at 1, 3 and 8 days past inoculation (dpi). In total, 71,569 transcripts were identified, and 10,929 of them were examined as differentially expressed genes (DEGs) in response to CCN infection. Based on the functional annotation and orthologous findings, the protein phosphorylation, oxidation-reduction process, regulation of transcription, metabolic process, transport, and response process as well as many other pathways previously reported were enriched at the transcriptional level. Plant cell wall hydrolysis and modifying proteins, auxin biosynthesis, signalling and transporter genes were up-regulated by CCN infection to facilitate penetration, migration and syncytium establishment. Genes responding to wounding and jasmonic acid stimuli were enriched at 1 dpi. We found 16 NBS-LRR genes, 12 of which were down-regulated, indicating the repression of resistance. The expression of genes encoding antioxidant enzymes, glutathione S-transferases and UDP-glucosyltransferase was significantly up-regulated during CCN infection, indicating that they may play key roles in the compatible interaction of wheat with CCN. Taken together, the results obtained from the transcriptome analyses indicate that the genes involved in oxidation-reduction processes, induction and suppression of resistance, metabolism, transport and syncytium establishment may be involved in the compatible interaction of Wen 19 with CCN. This study provides new insights into the responses of wheat to CCN infection. These insights could facilitate the elucidation of the potential mechanisms of wheat responses to CCN.
Forfattere
Inge Stupak Tat Smith Nicholas Clarke Teodorita Al-Seadi Lina Beniušienė Niclas Scott Bentsen Quentin Cheung Virginia Dale Jinke van Dam Rocio Diaz-Chavez Uwe Fritsche Martyn Futter Jianbang Gan Kaija Hakala Thomas Horschig Martin Junginger Yoko Kitigawa Brian Kittler Keith Kline Charles Lalonde Søren Larsen Dagnija Lazdina Thuy P. T. Mai-Moulin Maha Mansoor Edmund Mupondwa Shyam Nair Nathaniel Newlands Liviu Nichiforel Marjo Palviainen John Stanturf Kay Schaubach Johanny Arilexis Perez Sierra Vita Tilvikiene Brian Titus Daniela Thrän Sergio Ugarte Liisa Ukonmaanaho Iveta Varnagiryte-Kabasinskiene Maria WellischSammendrag
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Sammendrag
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Sammendrag
Humic substances are important indicators of soil fertility. The fluorescence properties of humic acids from black soils in Harbin, northeast China, were investigated, after long-term fertilization using treatments with or without mineral fertilizer (NPK) and organic manure. Excitation and emission matrices combined with parallel factor analysis were used to investigate the structure of the humic acid. Principal component analysis was performed to select the most suitable parameters for the description of humic acid. The dimension reduction for the original fluorescence parameters extracted two principal components. By using the two principal component scores as a new index for clustering, it was concluded that long-term fertilization treatments in black soil in Harbin clustered into three groups of manure + NPK and organic manure treatments, NPK treatment, and soil without any fertilization. Manure + NPK fertilization and manure fertilization alone led to a higher degree of humification than NPK only or the control. We conclude that long-term fertilization with organic matter with or without NPK could increase the humification degree of these soils.
Sammendrag
The objective of this study was to make an overview assessment of the potential effects of intensified forest management, promoted by the Norwegian government as a climate mitigation measure, on water quality in Norwegian surface waters. This study evaluated the following measures for forest intensification: (i) afforestation, (ii) intensification of planting and (iii) nitrogen fertilization shortly before harvest. A substantial literature review was made and a further development of the DWARF- framework tailored for Norwegian conditions provided the base for the study. The assessments were made based on the potential effects after forest harvest, using different management strategies like stem-only harvest and whole-three harvest. The potential effects were analysed on multiple parameters with focus on acidification, eutrophication, heavy metals, and carbon sequestration. The study used temporal resolution to address what effects the forest management practices might lead to 1, 10 and 100 years after harvest. This study concludes that there will be trade-offs between transitioning to a low carbon society and water quality, and the severity of effects may differ if they are evaluated on an annual, decadal or century scale.
Sammendrag
Det er ikke registrert sammendrag