Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2018
Authors
Jiska Joanneke van Dijk Graciela Rusch Hilde Karine Wam Paul Eric Aspholm Even Bergseng Vegard GundersenAbstract
No abstract has been registered
Authors
Even Klyve Dahl Hetron Mweemba Munang'andu Andre van Eerde Jihong Liu Clarke Øystein EvensenAbstract
No abstract has been registered
Authors
Andre van Eerde Johanna Gottschamel Ralph Bock Kristine Eraker Aasland Hansen Hetron Mweemba Munangandu Henry Daniell Jihong Liu ClarkeAbstract
Dengue fever is a mosquito (Aedes aegypti) ‐transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1‐4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (CYD‐TDV), is available after many decades’ efforts, but only has moderate efficacy. More effective and affordable vaccines are hence required. Plants offer promising vaccine production platforms and food crops offer additional advantages for the production of edible human and animal vaccines, thus eliminating the need for expensive fermentation, purification, cold storage and sterile delivery. Oral vaccines can elicit humoral and cellular immunity via both the mucosal and humoral immune systems. Here, we report the production of tetravalent EDIII antigen (EDIII‐1‐4) in stably transformed lettuce chloroplasts. Transplastomic EDIII‐1‐4‐expressing lettuce lines were obtained and homoplasmy was verified by Southern blot analysis. Expression of EDIII‐1‐4 antigens was demonstrated by immunoblotting, with the EDIII‐1‐4 antigen accumulating to 3.45% of the total protein content. Immunological assays in rabbits showed immunogenicity of EDIII‐1‐4. Our in vitro gastrointestinal digestion analysis revealed that EDIII‐1‐4 antigens are well protected when passing through the oral and gastric digestion phases but underwent degradation during the intestinal phase. Our results demonstrate that lettuce chloroplast engineering is a promising approach for future production of an affordable oral dengue vaccine.
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No abstract has been registered
Authors
Synnøve Rivedal Samson Øpstad Torbjørn Haukås Johannes Deelstra Peter Dörsch Trond Børresen Sissel Hansen Sverre HeggsetAbstract
No abstract has been registered
Abstract
A number of factors such as low soil temperature, desiccation and thatch can be serious limiting factors for the successful reestablishment of golf greens following winter damages. The rate of germination and seedling root growth have important implications for competition between species on a golf green. This research project has shown that P. annua is a very competitive species, due to quicker germination at lower temperatures, especially compared to A. stolonifera and F. rubra ssp communtata. Root growth of P.annua was also significantly quicker than of the Agrostis species tested. Seedlings of Agrostis species and F.rubra ssp commutata that germinate in close proximity to P. annua seedlings stand a large chance of being choked out. In order to reduce competition with P.annua, early seeding should be avoided. In this study, no difference in turfgrass establishment wasobserved when seedlings were grown using soil water extracts or soil from an ice-encased green,compared to a control. However, further investigations regarding reestablishment following iceencasement are warrant, and should be investigated on older greens with a higher organic mattercontent. The results from the demonstration trials emphasize the importance of using a sowingtechnique that ensures proper seed – soil contact. This is of particular importance for theestablishment of turfgrass species on golf greens, due to the high risk of desiccation.
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2017
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No abstract has been registered