Lisa Karine Haugland

Avdelingsingeniør

(+47) 915 35 189
lisa.haugland@nibio.no

Sted
Ullensvang

Besøksadresse
Ullensvangvegen 1005, 5781 Lofthus

Sammendrag

Grey mold caused by the necrotrophic fungal pathogen Botrytis cinerea can affect leaves, flowers, and berries of strawberry, causing severe pre- and postharvest damage. The defense elicitor β-aminobutyric acid (BABA) is reported to induce resistance against B. cinerea and many other pathogens in several crop plants. Surprisingly, BABA soil drench of woodland strawberry (Fragaria vesca) plants two days before B. cinerea inoculation caused increased infection in leaf tissues, suggesting that BABA induce systemic susceptibility in F. vesca. To understand the molecular mechanisms involved in B. cinerea susceptibility in leaves of F. vesca plants soil drenched with BABA, we used RNA sequencing to characterize the transcriptional reprogramming 24 h post-inoculation. The number of differentially expressed genes (DEGs) in infected vs. uninfected leaf tissue in BABA-treated plants was 5205 (2237 upregulated and 2968 downregulated). Upregulated genes were involved in pathogen recognition, defense response signaling, and biosynthesis of secondary metabolites (terpenoid and phenylpropanoid pathways), while downregulated genes were involved in photosynthesis and response to auxin. In control plants not treated with BABA, we found a total of 5300 DEGs (2461 upregulated and 2839 downregulated) after infection. Most of these corresponded to those in infected leaves of BABA-treated plants but a small subset of DEGs, including genes involved in ‘response to biologic stimulus‘, ‘photosynthesis‘ and ‘chlorophyll biosynthesis and metabolism’, differed significantly between treatments and could play a role in the induced susceptibility of BABA-treated plants.

Sammendrag

Secured supply of apple fruit for industrial use, especially for production of cider was investigated. The research questions were; is it more land to be used for apple growing in the Hardanger region?, is it possible to develop an own growing concept for fruit for industrial use?, how is the economy in an own concept? And what quality demands for the raw material is needed? More land is available in the region and how much is dependent on which quality the producers demand. The input factors in a growing concept were evaluated for economically impact and price level for the different classes were the most important. Fruit of different origin (discarded at picking or at grading) were compared. Yield level was most likely the most important factor for the differences found. Prognose of amount available for industry was calculated based on models from other countries and found to have potential. Knowledge gaps were identified, and further work is needed to determine quality demands.