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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2009

Sammendrag

The effect of air pollution from the Petchenganickel industrial complex, northwestern part of the Kola Peninsula, on forest vegetation was studied by combining three dormant monitoring networks in Finland, Russia and Norway, comprising a total of 21 plots that were revisited in 2004. Chemical composition of precipitation was monitored during 2004–2005, and indicated continuing high deposition of heavy metals and SO2 in the border area. The cover of epiphytic lichens on the trunks of downy birch (Betula pubescens) and Scots pine (Pinus sylvestris) was severely affected by pollution, and there was also a consistent negative effect on the abundance and richness of lichens and bryophytes on the forest floor in a more limited area. The effects of pollution on crown condition and stand growth were weak or absent. This study is an important reference for evaluating the effects of the planned renovation of the smelter in Nikel.

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Sammendrag

Høydeutvikling ble studert i bestand gjennom uttak av stammeskiver fra overhøydetrær. Produksjonen beregnet vi etter omfattende registrering i relativt unge og tette bestand ved anlegg og revisjon seks år seinere. De permanente flatene besto av to eller tre ruter; ei kontrollrute (T0), og ei eller to ruter som ble tynnet, svakt (T1) og/eller sterkt (T2). Høydeutviklingen hos svartor samsvarte godt med boniteringskurver for svartor i Mellom-Europa og Sverige. Disse har totalalder som inngang, mens det i Norge er vanlig med alder i brysthøyde. Sammenligningen med de norske bonitetskurvene for bjørk og osp var god fra henholdsvis 20 og 10–15 år og framover, men før den tid gir de en underbonitering. Avvikene var noe større for gråor og ask innen samme tidsperioder....

Sammendrag

To identify differentially expressed genes of the white-rot fungus Heterobasidion parviporum subtractive cDNA libraries were constructed using suppressive subtraction hybridization (SSH) technique with RNA extracted from an advanced stage of decay area and from colonization front next to the reaction zone of the stem of a mature Norway spruce trees. Besides cytochrome P450s and proteins with unknown function, the SSH libraries constructed contained genes involved in basic cellular processes, andcell wall degradation. To examine the role of selected candidate genes three trees, showing a variable degree of wood decay, were used for real-time RT-PCR profiling of candidate genes. In the decay transition areas the study revealed activity centers that showed remarkable similarity in the transcript profiles of monitored genes.

Sammendrag

Due to the great economic losses caused by the root and butt-rot pathogen Heterobasidion annosum, development of efficient control measures is warranted. H. annosum a necrotroph colonize the Norway spruce from inside and is responsible for 100s of millions of Euros losses annually. Considerable clonal variation has been recorded for Norway spruce in resistance towards H. annosum, but the defence mechanisms contributing to host resistance against both necrotrophic and biotrophic fungi remain poorly understood. The recent genome sequencing of Populus has made the genus a model to facilitate tree genetics. Genome-wide transcript profiling of Populus tremula upon pathogen attack will now be used, and homologues of Norway spruce genes to defence genes up-regulated in Populus will be identified. Two aspen clones (23 and 72) from the SwAsp collection (Luquez 2007) were used in the present study. Plants were propagated from tissue culture and kept in greenhouse under un-manipulated conditions. To study the host defence mechanisms, the rust (Melampsora magnusiana Wagner) and a bluestain fungus were used as a biotrophic and necrotrophic fungus respectively. Melampsora spores solution was applied to the underside of the leaf. To control for sectoriality six leaves were infected on each plant. To ensure high humidity and avoid cross contamination, plastic bags were wrapped around infected leaves. Leaves above infected leaves (10, 9, and 8) were harvested respectively 1, 3 and 14 days after inoculation. Initial results from microarray data indicate a clear separation between two Aspen clone (23 and 72) lines. For line 23 the response to biotroph and necrotroph seems to be similar. Whereas the response for line 72 is divergent for the treatments as they go in opposite direction. The controls show that there is an initial difference in the 2 lines (controls are separated). What are the genes that make biotrohic and necrotrophic treatment of 72 look so different will be worked out from microarray data. Differential expression of defense genes in biotrohic and necrotrophic treatment will be verified further with quantitative real time PCR. Chemical analysis of Aspen leaves gave less phenolic compounds as plants were kept in greenhouse. HPLC will be carried out after reaching some conclusion from microarray data analysis.

Sammendrag

The genome sequence of the conifer rot root pathogen Heterobasidion annosum was generated at JGI with 8.23 X coverage. The nuclear genome assembles in 39 scaffolds of total 33.7 Mbp estimated to cover 98.1% of the complete genome. We predicted 12,270 genes with an average length of 1,617 bp and exon number and length of 5.54 and 250 bp respectively. About 50% (5999) of the predicted genes could be validated by EST support with the 40,807 EST´s generated with in the project. The genome has a GC content of 52.0% and very little repetitive sequences with 2,895 SSR per mega base. The physical genome is congruent with the genetic linkage map, and most of the linkage groups have been possible to anchor to the 18 largest scaffolds.

Sammendrag

Increased urbanization in many societies is having a negative impact on vitality of rural areas. To maintain the vitality of these areas governments have employed a variety of policies, some of which are designed to increase new and alternative income from forest land. The multi-dimensional value of natural resources creates possibilities for local economic development, and landowners are a key factor in releasing this potential. Innovation research within the use of forest land has experienced rejuvenation in recent years as reduced income from agricultural products, saw logs and pulp logs has become a critical policy issue at national and regional levels. Four innovation-oriented studies have recently been completed at the University of Life Science and the Forest and Landscape Institute in Norway. These studies examined entrepreneurial attitudes effect on probability for start-ups entrepreneurial attitudes impact on innovation and performance social networks, learning orientation and entrepreneurial climate impact on innovativeness. This presentation will highlight the most important findings from these studies and outline implications of the findings that may be used by policy makers, landowners and research.

Sammendrag

MicroRNAs (miRNAs) are endogenous small RNAs that can have large-scale regulatory effect and could participate in epigenetic regulation of gene expression in plants. We show for the first time that temperature during zygotic embryogenesis and seed maturation in Norway spruce regulates an “epigenetic memory” in the progeny, regenerated through somatic embryogenesis. The warmer the in vitro temperature applied, the later the regenerated plants formed terminal buds in the common environment the second growth season. The differences were very large, and similar in size to a provenance separation of 4 – 6 degrees of latitude (Kvaalen and Johnsen, 2008). To study a molecular mechanisms of a memory from embryo development we have prepared two concatemerized small RNA libraries representing small RNAs predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (CEL) and warm environment (WEL) after short day (SD) treatment (going to bud set). In total we obtained 201 different small RNAs, with dominated length of 21-nt, 123 from WEL and 93 from CEL. Using multiple methods, including BLAST, sequence alignment and sequence folding we found 27 novel candidate miRNAs and only 3 earlier described. Additionally 103 small RNAs have exact matches in spruce EST database, which could be their putative targets and 67 small RNAs have no matches. We used quantitative RT–PCR to study the expression patterns of 31 chosen candidate miRNAs and monitor the occurrence stage-dependent miRNA-mediated cleavage for 4 regions of putative mRNA targets. All miRNA show difference in transcript levels after SD treatment and 12 miRNAs show constitutively differential expression in progeny from CE and WE. Kvaalen H, Johnsen O (2008) Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytologist 177: 49-59

Sammendrag

Historically, the forest sector has been an important industry for the Norwegian economy, particularly for rural areas. Even though growth patterns among rural communities have differed, all have been greatly affected by urban centralization, global markets, and economic decline in the agricultural sector. The multi-dimensional value of natural resources creates possibilities for local economic development, and landowners are a key factor in releasing this potential. Innovation research within the use of forest land has experienced rejuvenation in recent years as reduced income from agricultural products, saw logs and pulp logs has become a critical policy issue at national and regional levels. Four innovation-oriented studies have recently been completed at the University of Life Science and the Forest and Landscape Institute in Norway. These studies examined Entrepreneurial attitudes effect on probability for start-upsEntrepreneurial attitudes impact on innovation and performancesocial networks, learning orientation and entrepreneurial climate impact on innovativeness. This presentation will highlight the most important findings from these studies and outline implications of the findings that may be used by policy makers, landowners and research.