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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2009

Sammendrag

Omtale av epleviklermodellen som Marc Trapman har lagd i RIMpro. Denne er nå lagt ut på VIPS for å få erfaringer, men den er ikke validert for norske forhold.

Sammendrag

Bilberry (Vaccinium myrtillus), also called European blueberry, is one of the most significant wild berries in the Nordic countries. The berries are recognized for their bioactive properties and distinctive aroma and flavor. The effects of climate on production and the quality of bilberries have been studied in a controlled experiment in a phytotrone using clonal material. In the experiment individual plants from two Northern clones and two Southern clones of bilberry have been grown at 12◦ and 18◦ C. At each temperature 3 different light treatments have been tested; 1) 24 hour (h) natural light (long day); 2) 24 h natural light with addition of red light and 3) 12 h light (short day). All plants were kept outside during flowering to ensure pollination by insects. Berries were sampled when ripe, weighed and stored at -80◦C for later analysis of carbohydrates, acids, phenols, anthocyanins and ascorbic acid. At 12◦C the two Northern clones produced the first ripe berries 5 weeks after pollination, while the two Southern clones needed an extra two weeks to ripening. This difference was most evident at the 24 hour day length treatment. At 18◦C both the Northern and Southern clones had ripe berries from 5 weeks after pollination. The results also indicate that the Northern clones produce better at long days, with and without addition of red light, compared to the Southern clones. The levels of fructose, glucose and sucrose are higher in berries developed at 12 than 18◦ C (means of all treatments and all clones). Also the level of citric acid, quinic acid, myo-inositol, gallic acid, cathecin, epigallocatechin, catechin derivates and quercetin are higher at the lowest temperature.

Sammendrag

Plant polyphenolics continue to be the focus of attention with regard to their putative impact on human health. An increasing and ageing human population means that the focus on nutrition and nutritional enhancement or optimization of our foodstuffs is paramount. Using raspberry as a model we have shown how modern metabolic profiling approaches can be used to identify the changes in the level of beneficial polyphenolics in fruit breeding segregating populations and how the level of these components are determined by genetic and/or environmental control. Interestingly the Vitamin C content appeared to be significantly influenced by environment (growth conditions) whilst the content of the polyphenols such as cyanidin, pelargonidin and quercetin glycosides appeared to much more tightly regulated suggesting a rigorous genetic control. Preliminary metabolic profiling showed that the fruit polyphenolic profiles divided into two gross groups segregating on the basis of relative levels of cyaniding-3-sophoroside and cyaniding-3-rutinoside, compounds implicated as conferring human health benefits.

Sammendrag

Hykor raisvingel kan nyttast som rein sort eller i blanding med andre sortar. Unntaket er timotei som vil gå fort ut i lag med Hykor. Sorten overvintrar godt og startar veksten tidleg om våren. Førsteslåtten har få aks som skyt tidleg og den raske gjenveksten er kun blad. Stor avling og tidleg slått gjer at det ikkje bør gå meir enn 5-6 veker mellom slåttane, både for å utnytta det store gjenvekstpotensialet og for å oppnå ein akseptabel fôrkvalitet.

Sammendrag

Plantevernleksikonet. Skadegjørere: Bærtege Jordbærtege Jordbærmellus Skumsikade Ripsrotlus Liten stikkelsbærbladlus Liten bringebærbladlus Liten ripsbladlus Liten solbærbladlus Bjørnebærgrasbladlus Lauvtrebille Knoppsnutebille Jordbærsnutebille Bringebærbille Stikkelsbærpyralide Stikkelsbærmåler Bringebærmøll Ripsskuddmøll Ripsglassvinge Bringebærglassvinge Bringebærbarkgallmygg Solbærgallmygg Bringebærgallmygg Bringebærflue Stikkelsbærbladveps Bringebærbladveps Gul bjørnebærbladveps Jordbærmidd Solbærgallmidd Bjørnebærbladmidd Solbærbladmidd For hver skadegjører gis en oversikt over utseende, utbredelse, vertplanter, livssyklus, skadevirkninger og bekjempelse. 

Sammendrag

The application of genetic engineering in the floriculture industry has become instrumental and rewarding in meeting the demand for novel desirable traits. Agrobacterium-mediated transformation for poinsettia (Euphorbia pulcherrima Willd. Ex Klotzsch), a contemporary symbol of Christmas in most parts of the world, is reported here for the first time. Internode stem explants of poinsettia cv. Millenium were transformed by Agrobacterium tumefaciens, strain LBA 4404, harbouring hairpin (hp) RNA gene constructs to induce RNA silencing-mediated resistance to Poinsettia mosaic virus (PnMV). In five experiments utilizing 868 explants, 18 independent transgenic lines were generated. Stable integration of transgenes into the poinsettia nuclear genome was confirmed by PCR and Southern blot analysis. Both single- and multiple-copy transgene integration into the poinsettia genome were found among transformants. Transgenic poinsettia plants showing resistance to mechanical inoculation of PnMV were detected by double antibody sandwich enzyme-linked immunosorbent assays. Northern blot analysis of low molecular weight RNA revealed that transgene-derived small interfering (si) RNA molecules were detected among the poinsettia transformants prior to inoculation. The Agrobacterium-mediated transformation methodology developed in the current study should facilitate improvement of this ornamental plant with enhanced disease resistance, quality improvement and desirable colour alteration. Production of yellow-coloured poinsettia using the Agrobacterium-mediated transformation method is in progress.  

Til dokument

Sammendrag

Genetic instability could be provoked as an unintended consequence of genetic engineering in plants. Here we report that the rice endogenous long terminal repeat (LTR) retrotransposon Tos17 was transpositionally activated only in transgenic calli and their regenerated plants produced by biolistic transformation in rice (Oryza sativa L.) ssp. japonica cv. Matsumae. Moreover, the transpositional activity of Tos17 was sustained after plant regeneration in the T0 generation, and produced new germinal insertions. In contrast, the element remained totally quiescent in calli and regenerated plants from tissue culture of this genotype. Nonetheless, transcriptional induction and cytosine demethylation of Tos17 were found to have occurred with no significant difference in both kinds of calli, tissue culture alone and transgenic. This suggests that callus culture is likely to have played an important role in destabilizing Tos17 in the direction towards transpositional activation, but that biolistic transformation is the direct causal factor.

Til dokument

Sammendrag

Genetic instability could be provoked as an unintended consequence of genetic engineering in plants. Here we report that the rice endogenous long terminal repeat (LTR) retrotransposon Tos17 was transpositionally activated only in transgenic calli and their regenerated plants produced by biolistic transformation in rice (Oryza sativa L.) ssp. japonica cv. Matsumae. Moreover, the transpositional activity of Tos17 was sustained after plant regeneration in the T0 generation, and produced new germinal insertions. In contrast, the element remained totally quiescent in calli and regenerated plants from tissue culture of this genotype. Nonetheless, transcriptional induction and cytosine demethylation of Tos17 were found to have occurred with no significant difference in both kinds of calli, tissue culture alone and transgenic. This suggests that callus culture is likely to have played an important role in destabilizing Tos17 in the direction towards transpositional activation, but that biolistic transformation is the direct causal factor.