Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
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Perennial ryegrass (Lolium perenne L.) is not widely used in forage production in Norway; until recently only in regions with very mild winter climate. Due to its high digestibility and yield potential, and trends towards milder winters, the interest for using this species in silage production has increased. However, variable winter weather with frost and ice can damage perennial ryegrass extensively, and it is therefore regarded as a rather short-lived species under these conditions. In this paper, we report results from field experiments for first-year leys established in 2016 at three different locations from south to north in Norway. Different seed mixtures of grass and clover species were sown with and without the addition of perennial ryegrass. In 2017, plots were fertilised with either medium level of nitrogen (N) or low N-level (half of the medium level). Dry matter yields, botanical composition and feed quality (determined by NIRS) from each cut were recorded. Perennial ryegrass dominated in all mixtures and reduced weed invasion, regardless of location. Inclusion of ryegrass led to higher yield production compared to mixtures without ryegrass; it increased digestibility but the content of crude protein tended to be lower, probably due to a dilution effect caused by the higher yield production.
Forfattere
Åshild Taksdal Randby Astrid Johansen Linda Rønning Linda KarlssonSammendrag
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Igor A. Yakovlev Daniel Gackowski Abdulkadir Abakir Marcos Viejo Alexey Ruzov Ryszard Olinski Marta Starczak Carl Gunnar Fossdal Konstantin V. KrutovskySammendrag
5-Methylcytosine (5mC) is an epigenetic modification involved in regulation of gene expression in metazoans and plants. Iron-(II)/α-ketoglutarate-dependent dioxygenases can oxidize 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Although these oxidized forms of 5mC may serve as demethylation intermediates or contribute to transcriptional regulation in animals and fungi, experimental evidence for their presence in plant genomes is ambiguous. Here, employing reversed-phase HPLC coupled with sensitive mass spectrometry, we demonstrated that, unlike 5caC, both 5hmC and 5fC are detectable in non-negligible quantities in the DNA of a conifer, Norway spruce. Remarkably, whereas 5hmC content of spruce DNA is approximately 100-fold lower relative to human colorectal carcinoma cells, the levels of both - 5fC and a thymine base modification, 5-hydroxymethyluracil, are comparable in these systems. We confirmed the presence of modified DNA bases by immunohistochemistry in Norway spruce buds based on peroxidase-conjugated antibodies and tyramide signal amplification. Our results reveal the presence of specific range of noncanonical DNA bases in conifer genomes implying potential roles for these modifications in plant development and homeostasis.
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Einar StrandSammendrag
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Harald Bratli Rune Halvorsen Anders Bryn Geir Arnesen Egil Bendiksen Jon Bjarne Jordal Ellen Johanne Svalheim Vigdis Vandvik Liv Guri Velle Dag-Inge Øien Per Arild AarrestadSammendrag
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