Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2006
Sammendrag
Cereal cyst nematodes, Heterodera spp., are known worldwide as parasites of cereals and grasses. Surveys of cereal fields in Norway have revealed that nematodes belonging to the H. avenae complex occur throughout the country, and that H. avenae (the oat cyst nematode) is the most common species, followed by H. filipjevi (the rye cyst nematode). Both species are of economic importance in Scandinavia. H. avenae has been found in two common pathotypes, Ha 11 and Ha 12. Work in Sweden, however, has detected three additional pathotypes, H. avenae "Knislinge", H. avenae "Ringsåsen" and H. avenae "Våxtorp". These pathotypes were found also in the Norwegian surveys. In Sweden H. filipjevi has two pathotypes, "East" and "West". In Norway, only pathotype "West" has been detected so far. Nematode management practices must be based on the knowledge of the relationship between initial nematode density and yield, the population dynamics, and the measures capable of reducing or keeping the population density below the threshold for economic damage. Crop rotation and the use of cultivars with resistance are important measures for controlling cereal cyst nematodes. Several cereal cultivars with resistance to H. avenae are on the market. As to H. filipjevi, resistance may be found in some commercial cultivars, although no intentional breeding for resistance against this nematode species has been attempted. In 2004 and 2005 the majority of the cereal cultivars on the Norwegian market were tested for susceptibility/resistance towards H. avenae pathotype Ha 11, H. avenae pathotype "Våxtorp" and H. filipjevi pathotype "West". Management systems, based on careful nematode identification and good knowledge of appropriate resistant cultivars, are in operation in Norway. Resistant barley is generally recommended when nematode populations are high due to its high tolerance compared to resistant oats. Farmers implementing this program have reported increased cereal yields on the average of 1000 kg /ha. It has been calculated that by implementing this program in full the county of Vestfold could make an economic gain of 800 000 " annually.
Sammendrag
We describe the cloning and characterization of a single copy gene from Trichoderma atroviride P1 encoding a novel 30 kDa chitinase, Ech30. Ech30 is a family 18 chitinase showing low sequence similarity to other Trichoderma chitinases. Real-time quantitative RT-PCR studies revealed that expression of the ech30 gene was induced by the presence of Botrytis cinerea in plate confrontation assays, but hardly by chitin in liquid cultures. Studies of Ech30 purified from an Escherichia coli strain overexpressing the ech30 gene devoid of the leader sequence and a predicted intron, showed that the gene encodes an active chitinase, which, as expected for family 18 chitinases, is inhibited by allosamidin.
Forfattere
Christer MagnussonSammendrag
Det er ikke registrert sammendrag
Sammendrag
Effects of composting on survival and reproduction of the potato cyst nematode (PCN) Globodera rostochiensis were investigated in 150-liter pilot scale reactors. Small bags containing cysts of PCN were incubated in organic waste and composted for 8, 15 or 29 days. Temperatures in the areas close to the cyst bags were monitored. Survival, measured by hatching, was reduced by 99.9% already after 8 days of composting and at 29 days no hatching was recorded. A few eggs, however, still hatched after being composted for 15 days and exposed to temperatures higher than 60°C for several days. Maximal temperatures and thermal exposure time as such could not explain the hatching pattern of PCN, so other factors are likely to be involved in causing lethality. PCN failed to reproduce after 8 days of composting with temperatures reaching a minimum of 50°C during the period. Hence, eight days of composting where all material have reached a temperature of minimum 50°C during the period, could be regarded as a satisfactory method for sanitation of PCN.
Forfattere
Christer MagnussonSammendrag
Det er ikke registrert sammendrag
Forfattere
jihong liu clarke Sonja Klemsdal Erling Fløistad A.K. Hvoslef-Eide Sissel Haugslien Roe Moe Dag-Ragnar BlystadSammendrag
Poinsettia, Euphorbia pulcherrima Willd & Klotsch, is an ornamental flower which grows in many countries. Two viruses i.e. poinsettia mosaic virus (PnMV) and poinsettia cryptic virus (PnCV) can cause diseases on modern poinsettia cultivars. To produce virus-resistant poinsettia plants, genetic transformation has been considerated. Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresis-based transformation method to generate poinsettia transformants. The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure. To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene. Here, we report our preliminary results.
Forfattere
jihong liu clarke Sonja Klemsdal Erling Fløistad A.K. Hvoslef-Eide Sissel Haugslien R Moe Dag-Ragnar BlystadSammendrag
Poinsettia (Euphorbia pulcherrima) is a major ornamental pot plant in many countries. Two viruses, poinsettia mosaic virus (PnMV) and poinsettia cryptic virus, often infect modern poinsettia cultivars. To produce virus-resistant poinsettia plants, genetic engineering has been considered. Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresisbased transformation method to generate poinsettia transformants. The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure. To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene. Here we report our preliminary results.
Forfattere
Solveig HaukelandSammendrag
Det er ikke registrert sammendrag
Forfattere
Solveig HaukelandSammendrag
The black vine weevil (Otiorhynchus sulcatus) is an important pest in many crops in several countries worldwide. The adults feed on the leaves of strawberries, rhododendrons, azaleas and many other ornamental plants. Indoors, O. sulcatus can infest begonias, ferns, cyclamins, and other common container plants. Adult feeding does not seriously affect the plants, it is the larval feeding on roots of host plants that causes the most serious plant injury. Larvae may also girdle the main stem below the soil line. Leaves will yellow and plants may be stunted or die. O. sulcatus weevils are difficult to control once established because of their nocturnal behaviour and the subterranean habits of the larvae. In strawberries chemical control using a pesticide against the adult weevils after harvest is the most common control method today, however the most effective pesticides will shortly no longer be available and there are few alternative pesticides on the market. Fortunately there are Entomopathogenic nematodes (EPN) that can be used to control the soil dwelling larvae and in Europe there are several products on the market comprising three species (Heterorhabditis bacteriophora, H. megidis, and Steinernema kraussei). The use of these nematodes has shown to be quite successful in potted plants (in nurseries), whereas in field grown crops such as strawberry they are still not consistently effective. In this presentation results from a number of field trials using H. megidis and S. kraussei against O. sulcatus larvae infesting field grown strawberry will be presented and discussed.
Forfattere
Solveig HaukelandSammendrag
Det er ikke registrert sammendrag