Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2004
Forfattere
Helge Sjursen Jan NetlandSammendrag
Forsøkene i dette brukerstyrte prosjektet, i samarbeid med Lier og omegn forsøksring, og med midler fra SND, har vist at damping av jord i ulike salatkulturer (isbergsalat, kinakål, spinat og ruccola) på friland er velegnet for å redusere innholdet av ugrasfrø (frøbanken) i det 0-5 cm øvre laget i jorda. Det ble brukt dampemaskin av merket "Regero", produsert i Frankrike. Ved damping i minst 2½ minutt (svarende til 75% av "normal" dampestyrke) ble det oppnådd bortmot 100o C av varierende lengde, men det er trolig lengden på temperaturperioder over 70o C som er avgjørende for å drepe ugrasfrøene. Den sanerende effekten av dampingen på ugrasfrøbanken ga markert redusert ugrasframvekst og økt dekningsgrad av kulturene i forhold til ubehandla ledd.
Forfattere
Gunnhild Jåstad Geir Kjølberg Knudsen Sverre Kobro Anna-Carin Bäckman Peter Witzgall Marie BengtssonSammendrag
Rognebærmøll, Argyresthia conjugella Zell. (Lepidoptera: Argyresthiidae), er eit av dei viktigaste skadedyra i eple i Skandinavia. I år då hovudvertsplanta til rognebærmøllet, rogna, har lite eller ikkje bær fyk hoene inn i eplehagane for å leggje egg. I enkelte år kan heile epleavlingane verte totalskada. flyktige luktstoff frå rogn og eple har vorte samla og identifisert. I GC-ED testar har hoer reagert på fleire komponentar som er funne i både rogn og eple. Nokre av desse komponentane vart nytta i felle forsøk i felt i 2002, og ei blanding av to komponentar fanga signifikant fleire av både hoer og hannar samanlikna med kontrollfeller. Resultat frå sesongen 2003 viste at to-komponent blandinga fanga insekta for seint i sesongen i høve til å kunne hindre egglegging i eple. Fleire nye komponentar vart testa ut i 2003, og nokre av desse gav lovande resultat. Desse resultata vil verte diskutert i høve til å bruke tiltrekkjande planteluktstoff som eit kontroll tiltak mot rognebærmøll.
Forfattere
Tore Skrøppa Øystein Johnsen Carl Gunnar Fossdal Rudiger Baumann Jørgen A. Mølmann Ola G Dæhlen Nina Elisabeth Nagy G Müller-StarckSammendrag
Det er ikke registrert sammendrag
Forfattere
Carl Gunnar Fossdal Øystein Johnsen Rüdiger Baumann Jørgen A. Mølmann Ola Gram Dæhlen Nina Elisabeth Nagy Tore SkrøppaSammendrag
Research indicate that the female parents of Norway spruce adjust these timing events in their progeny according to the prevailing temperature conditions during seed development. Reproduction in a cold environment advances bud-set and cold acclimation in the autumn and dehardening and flushing in spring, whereas a warm reproductive environment delays these progeny traits by an unknown non-Mendelian mechanism. We have performed identical crosses in combination with timed temperature treatments during shorter and longer periods from female meiosis, pollen tube growth, syngamy and embryogenesis, tested the progenies for bud-set and frost hardiness, and concluded that the effect of temperature most likely is a response to accumulated heat during embryogenesis and seed maturation. Our first attempt to look for a molecular mechanism has revealed that transcription of PHYO, PHYP and PHYN and the class IV chitinase PaChi4 (using RealTime PCR) all show higher transcription levels in progenies born under cold conditions than their full-sibs born under warmer conditions. This result is consistent with preliminary findings that methylation of cytosine in total DNA is higher in progenies reproduce under warm conditions than their colder full-sib counterparts. If these observations are related to methylation, we may explain why progenies with a memory of a past time cold embryogenesis are more sensitive to short days than their full-sibs with a warmer embryonic history.
Sammendrag
Introduction: The objectives of the present study were to monitor H. annosum colonization rate (Hietala et al., 2003) and expression of host chitinases in clonal Norway spruce material with differing resistances. Transcript levels of three chitinases, representing classes I, II and IV, were monitored with real-time PCR.Material and MethodsInoculation experiment: Ramets of two 32 -year-old clones differing in resistance were employed as host material. Inoculation and wounding was performed. A rectangular strip containing phloem and cambium, with the inoculation site in the middle, was removed 3, 7 and 14 days after inoculation.Quantification of fungal colonizationMultiplex real-time PCR detection of host and pathogen DNA was performed (Hietala et al., 2003). Quantification of gene expression: Chitinase levels were monitored with Singleplex real-time PCR.Results and ConclusionsThe colonization profiles provided by the quantitative multiplex real-time PCR procedure (Hietala et al., 2003), when combined with spatial and temporal transcript profiling of 3 chitinases, provide a useful basis for identifying defense related genes, and for assessing their impact on pathogen colonization rates.Three days after inoculation, comparable colonization levels were observed in both clones in the area immediately adjacent to inoculation. Fourteen days after infection, pathogen colonization was restricted to the area immediately adjacent to the site of inoculation for the strong clone (589), but had progressed further into the host tissue in the weak (409) clone.Transcript levels of the class II and IV chitinases increased following wounding or inoculation, while the transcript level of the class I chitinase declined following these treatments. Transcript levels of the class II and class IV chitinases were higher in areas immediately adjacent to the inoculation site in 589 than in similar sites in 409 three days after inoculation, suggesting that the clones differ in the rate of chitinase-related signalperception.
Sammendrag
We have monitored the H. annosum colonization rate and expression of host chitinases in Norway spruce material with differing resistances. Transcript levels of three chitinases, representing classes I, II and IV, were monitored with real-time PCR. Ramets of two 32 -year-old clones differing in resistance were employed as host material and inoculation and wounding was performed. Quantification of fungal colonization: Multiplex real-time PCR detection of host and pathogen DNA was performed. Chitinase transcript levels were also monitored with real-time PCR. Three days after inoculation, comparable colonization levels were observed in both clones in the area immediately adjacent to inoculation. Fourteen days after infection, pathogen colonization was restricted to the area immediately adjacent to the site of inoculation for the strong clone (589), but had progressed further into the host tissue in the weak clone (409). Transcript levels of the class II and IV chitinases increased following wounding or inoculation, while the transcript level of the class I chitinase declined following these treatments. Transcript levels of the class II and class IV chitinases were higher in areas immediately adjacent to the inoculation site in 589 than in similar sites in 409 three days after inoculation, suggesting that the clones differ in the rate of chitinase-related signal perception. The spatiotemporal accumulation patterns obtained for the two clones used are consistent with their resistance classifications, these warranting further and more detailed studies on these chitinases.
Sammendrag
The sun is an abundant energy source, and increasing efforts are made to find more efficient ways to exploit it, than commonly used today. Hydrogen is considered to be the energy carrier of the future, and the potential for a sustainable system where hydrogen is obtained directly from solar energy, has been studied extensively. One alternative is the process of biophotolysis. Sulfur starvation of the green algae Chlamydomonas reinhardtii is known to cause hydrogen production under illumination, by biophotolysis where solar energy is used to produce significant amounts of hydrogen involving parts of the photosynthetic process. So far, little is known about this process in other species, and in this work we have investigated different species of green algae with respect to hydrogen production under sulfur starvation. A number of algae cultures were screened with respect to physiological response to sulfur deprivation in small-scale laboratory cultures under controlled conditions. Test parameters included hydrogen production, reduction of oxygen production, changes in morphology and other aspects of physiology. Investigations of oxygen sensitivity of hydrogenases were also performed. It was shown that other species than C. reinhardtii are able to produce hydrogen under sulfur deprivation.
Sammendrag
Most energy carriers that are in common use today originate from solar energy. Hydrogen is considered to be the energy carrier of the future, and the potential for a sustainable system where hydrogen is obtained directly from solar energy, has been studied by several researchers over the years. Several groups of microorganisms have shown the ability to produce hydrogen by natural biological processes using solar energy. Efforts have been made to understand the mechanisms involved in photobiological hydrogen production from these organisms, and to optimise the process. This work has recently resulted in a significant breakthrough. It has been discovered that some species of green algae have the ability to produce significant amounts of hydrogen during sulphur starvation, which allows hydrogen to be produced in light. However, very little is known about how this process varies between species. We have chosen to investigate green algae, with the intention to examine a variety of species for hydrogen production during sulphur starvation. A number of algae cultures were screened with respect to physiological response to sulphur deprivation in small-scale laboratory cultures under controlled conditions. Results from both marine and fresh water algae will be presented.
Sammendrag
Tørråte i potet krever i de fleste potetdistrikter mange sprøytinger for å holdes under kontroll. I likhet med de siste årene kommer vi med en omtale av varsling og middelvalg i forbindelse med bekjempelsen av denne viktige skadegjøreren. Artikkelen er en oppdatering av tilsvarende artikler fra i fjor.
Sammendrag
Det er ikke registrert sammendrag