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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2009

Sammendrag

Continuous light is a promising method to reduce the problems with rose powderymildew in greenhouse rose production. The effects of such a light regime on the performance of insect pests on roses have so far not been investigated. In the present study, survival, developmental time, and reproduction during one generation of the greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), were characterized on roses, Rosa x hybrida cv. Passion, grown in climate chambers with long-day conditions (L20:D4) or continuous light (L24:D0) at 21 oC and fluctuating relative humidity (mean 74%, range 47–96%). Whiteflies reared under continuous light had lower immature survival and fecundity and shorter female longevity than whiteflies reared under long-day conditions, but immature developmental time was only slightly affected. Life-table analysis showed that the net reproductive rate (Ro) and intrinsic rate of natural increase (rm) were reduced by 85 and 76%, respectively, and the time for the population to double its size (D) was 4.2 times longer under continuous light. Thismean that the whitefly population growth under continuous light was strongly reduced compared with the traditional light regime used in rose production.

Til dokument

Sammendrag

The relative effects of using light (2-3 Mg) versus heavier (5-7 Mg) tractors, shallow (15 cm) versus deeper (25 cm) ploughing and on-land versus in-furrow wheel placement during ploughing were investigated from 2003 to 2006 in organic rotations (wheat or barley, green manure, oats with peas) and conventionally fertilized barley. Trials were located on loam soil in south-eastern Norway and silty clay loam in central Norway. Ploughing was performed in spring, when the topsoil moisture content was at or below field capacity, using single furrow ploughs that allowed alternative wheel placement and resulted in complete coverage of the surface by wheels each year (ca. 3 times the normal coverage during ploughing). Low tyre inflation pressures (:<= 80 kPa) were used throughout. The use of a heavy tractor increased topsoil bulk density slightly in the loam soil, and, in combination with in-furrow wheeling, it reduced air-filled pore space and air permeability at 18-22 cm. On the silty clay loam, the use of a heavy tractor did not increase bulk density, but it reduced air-filled pore space throughout the topsoil. In-furrow wheeling reduced air-filled pore space in this soil also, compared to on-land wheeling. Penetration resistance was in this soil always greater at 15-25 cm depth after shallow than after deep ploughing, especially with in-furrow rather than on-land wheeling. Shallow ploughing led on both soils to marked increases in perennial weed biomass compared to deep ploughing. Earthworms were hardly affected by the treatments, but in the loam in 2006 a higher number of individuals were found where the light rather than the heavy tractor had been used. Few significant treatment effects were found on grain yield and quality. Deep ploughing with a light tractor gave the highest wheat yield and protein content in 2 years on the loam soil, and on the silty clay loam the yield of conventionally fertilized barley was higher after deep than after shallow ploughing. In summary, limited evidence was found to support the use of on-land rather than in-furrow wheeling when ploughing is performed at favourable soil moisture and with tractor weights < 5 Mg. There is, however, reason to be wary of using heavy tractors (> 5 Mg), even under such conditions. With regard to ploughing depth in organic rotations dominated by cereals, the need to combat perennial weeds by deep ploughing weighs probably more heavily than any possible beneficial effect of shallow ploughing on stimulating nutrient turnover. (C) 2008 Elsevier B.V. All rights reserved.

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Sammendrag

In woody plants of the temperate zone short photoperiod (SD) leads to growth cessation. In angiosperms CONSTANS (CO) or CO-like genes play an important role in the photoperiodic control of flowering, tuberisation and shoot growth. To investigate the role of CO-like genes in photoperiodic control of shoot elongation in gymnosperms, PaCOL1 and PaCOL2 were isolated from Norway spruce. PaCOL1 encodes a 3.9 kb gene with a predicted protein of 444 amino acids. PaCOL2 encodes a 1.2 kb gene with a predicted protein of 385 amino acids. Both genes consist of two exons and have conserved domains found in other CO-like genes; two zinc finger domains, a CCT and a COOH domain. PaCOL1 and PaCOL2 fall into the group 1c clade of the CO-like genes, and are thus distinct from Arabidopsis CO that belongs to group la. Transcript levels of both PaCOL-genes appear to be light regulated, an increasing trend was observed upon transition from darkness to light, and a decreasing trend during darkness. The increasing trend at dawn was observed both in needles and shoot tips, whereas the decreasing trend in darkness was most prominent in shoot tips, and limited to the late part of the dark period in needles. The transcript levels of both genes decreased significantly in both tissues under SD prior to growth cessation and bud formation. This might suggest an involvement in photoperiodic control of shoot elongation or might be a consequence of regulation by light. (C) 2008 Elsevier Masson SAS. All rights reserved.

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Sammendrag

Conclusion: This is the first comprehensive experimental study showing that predicted SPs in the L. plantarum genome actually are capable of driving protein secretion. The results reveal considerable variation between the SPs that is at least in part dependent on the protein that is secreted. Several SPs stand out as promising candidates for efficient secretion of heterologous proteins in L. plantarum. The results for NucA provide some hints as to the sequence-based prediction of SP functionality, but the general conclusion is that such prediction is difficult. The vector library generated in this study is based on exchangeable cassettes and provides a powerful tool for rapid experimental screening of SPs.