Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2025
Sammendrag
NoBlight-prosjektet, leia av NIBIO, har som mål å auka kunnskapen kring tørrote og tørrflekksjuke, slik at vi kan utvikla nye verktøy og strategiar for nedkjemping, og dermed redusera bruken av sprøytemiddel. Eit av delmåla i prosjektet er å kartlegga tørrote-populasjonar i Noreg og undersøkja den genetiske variasjonen innan populasjonane
Forfattere
Theresa Weigl Jorunn Børve Emily Follett Melissa Magerøy Hanne Larsen Carl Gunnar Fossdal Siv Fagertun RembergSammendrag
The effect of harvest timing on postharvest ripening was investigated by changes in ethylene production, expression of ethylene biosynthesis genes MdACS1, MdACS6, (1-aminocyclopropane-1-carboxylic acid synthase 1 and 6) and the ACS degradation promoting gene MdETO1 (Ethylene overproducer 1). Apple fruit of two cultivars, ‘Red Aroma’ and ‘Rubinstep’, were harvested at three time points, early, middle, and late, at two-week intervals. Fruit were either treated with 1 ppm 1-Methylcyclopropene (1-MCP) or remained untreated, and stored at 4 °C in regular atmosphere. Late harvested, untreated apples reached peak ethylene production after the shortest time in cold storage ('Red Aroma' in week five, 'Rubinstep' in weeks nine and ten), while early harvested, untreated fruit reached their peak after a longer time ('Red Aroma' in week eight, 'Rubinstep' in weeks 13 and 14). Early harvested fruit experienced greater firmness loss and a higher increase in SCC/TA ratio during cold storage. Senescence in late harvested, untreated fruit was evident from low ethylene production after simulated shelf-life and increased physiological disorders in ‘Rubinstep’. In 1-MCP-treated fruit, ethylene production increased toward the end of storage, particularly in early harvested fruit, indicating a decline in 1-MCP efficacy over time. Gene expression analysis showed strong induction of MdACS1 during climacteric ripening. MdETO1 positively correlated with MdACS1 gene expression, suggesting positive co-regulation. The expression of MdACS6 was negatively correlated with simulated shelf-life and with 1-MCP treatment, suggesting regulation by temperature and metabolic state. Overall, harvest timing and 1-MCP strongly influenced the changes in fruit physiology during postharvest storage.
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
We report the successful cryopreservation of three economically important Rubus viruses: raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), and Rubus yellow net virus (RYNV), using shoot tip cryopreservation in four raspberry cultivars. Virus-infected shoot tips (approximately 1.0 mm in length) containing 3–4 leaf primordia (LPs) were cryopreserved using the droplet-vitrification technique. In the cultivars ‘Zlatá Královna (ZK)’ and ‘Tulameen (TUM)’, over 90% of shoot tips survived, and more than 90% regenerated into whole shoots. All three viruses were successfully preserved in the cryopreserved tissues, with recovery rates varying depending on virus type and cultivar: RBDV was recovered at rates of 86% in ‘ZK’ and 87% in ‘TUM’; BRNV at 66% in ‘ZK’ and 45% in ‘TUM’; and RYNV at 96%, 94%, and 86% in ‘Fairview’, ‘Stiora’, and ‘ZK’, respectively. To investigate viral localization in shoot tips, in situ hybridization was used. RBDV and RYNV infected a broad range of meristematic tissues, including the apical dome and LPs, whereas BRNV showed a more limited distribution. Virus distribution varied not only among virus species but also across raspberry cultivars, suggesting genotype-specific patterns of virus localization. Post-cryopreservation viral activity was verified using micrografting and aphid transmission assays. RBDV, BRNV, and RYNV were all successfully transmitted to healthy plants via micrografting, indicating the preservation of viral infectivity. Furthermore, BRNV was effectively transmitted by large raspberry aphids from cryopreserved materials, confirming vector-mediated transmission capacity post-thaw. Overall, this study demonstrates that shoot tip cryopreservation via droplet-vitrification is a reliable and effective strategy for preservation of biologically active Rubus viruses. This approach offers a valuable biotechnological tool for virus maintenance in support of diagnostic, breeding, and virology research.
Forfattere
Melissa Magerøy Veronica QT Phan Katherine Ann Gredvig Nielsen Paal Krokene Tage ThorstensenSammendrag
NIBIO i samarbeid med det norske oppstartsselskapet AgriBiotix AS undersøkt om naturlig forekommende bakterier som lever sammen med gran kan bidra til å holde plantene friske. I en norsk skogplanteskole isolerte vi ulike bakterier som beskytter granplantene mot gråskimmel. I flere tilfeller bidro også bakteriene til bedre plantevekst. Selv om det er behov for videre utvikling og godkjenning, kan slike gunstige bakterier bli et praktisk og bærekraftig tilskudd til integrert sykdomskontroll i skogplanteskolene.
Forfattere
Kirsten TørresenSammendrag
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